In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100422 |
Resumo: | ABSTRACT: Platelet-rich plasma (PRP) has been considered a promising therapeutic alternative, since platelets are rich in growth factors that are used in the Regenerative Medicine field. However, fresh PRP cannot be stored for long periods. This study aimed to develop a protocol for obtaining lyophilized canine PRP capable of maintaining viability after its reconstitution. For that purpose, canine PRP extraction and lyophilization protocols were initially tested. Subsequently, assays were carried out to quantify the growth factors VEGF and TGF-β, before and after the lyophilization process, gelation test and the three-dimensional gel structure analysis of the reconstituted lyophilized PRP by electron microscopy, as well as in vitro cell proliferation test in lyophilized PRP gel. Additionally, the immunogenicity test was performed, using allogeneic samples of lyophilized PRP. The results showed that the lyophilized PRP had adequate therapeutic concentrations of growth factors VEGF and TGF-β (9.1pg/mL and 6161.6pg/mL, respectively). The reconstituted PRP gel after lyophilization showed an in vitro durability of 10 days. Its electron microscopy structure was similar to that of fresh PRP. In the cell proliferation test, an intense division process was verified in mesenchymal stem cells (MSCs) through the three-dimensional mesh structure of the lyophilized PRP gel. The immunogenicity test showed no evidence of an immune reaction. The findings were promising, suggesting the possibility of having a lyophilized canine PRP that can be marketed. New in vivo and in vitro studies must be carried out for therapeutic confirmation. |
id |
EMBRAPA-2_17ec1d30ea39072bc8765ccafa2d4441 |
---|---|
oai_identifier_str |
oai:scielo:S0100-736X2021000100422 |
network_acronym_str |
EMBRAPA-2 |
network_name_str |
Pesquisa Veterinária Brasileira (Online) |
repository_id_str |
|
spelling |
In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic useIn-vitro productionlyophilizationcanineplatelet-rich plasmaregenerative medicinehealingdogsABSTRACT: Platelet-rich plasma (PRP) has been considered a promising therapeutic alternative, since platelets are rich in growth factors that are used in the Regenerative Medicine field. However, fresh PRP cannot be stored for long periods. This study aimed to develop a protocol for obtaining lyophilized canine PRP capable of maintaining viability after its reconstitution. For that purpose, canine PRP extraction and lyophilization protocols were initially tested. Subsequently, assays were carried out to quantify the growth factors VEGF and TGF-β, before and after the lyophilization process, gelation test and the three-dimensional gel structure analysis of the reconstituted lyophilized PRP by electron microscopy, as well as in vitro cell proliferation test in lyophilized PRP gel. Additionally, the immunogenicity test was performed, using allogeneic samples of lyophilized PRP. The results showed that the lyophilized PRP had adequate therapeutic concentrations of growth factors VEGF and TGF-β (9.1pg/mL and 6161.6pg/mL, respectively). The reconstituted PRP gel after lyophilization showed an in vitro durability of 10 days. Its electron microscopy structure was similar to that of fresh PRP. In the cell proliferation test, an intense division process was verified in mesenchymal stem cells (MSCs) through the three-dimensional mesh structure of the lyophilized PRP gel. The immunogenicity test showed no evidence of an immune reaction. The findings were promising, suggesting the possibility of having a lyophilized canine PRP that can be marketed. New in vivo and in vitro studies must be carried out for therapeutic confirmation.Colégio Brasileiro de Patologia Animal - CBPA2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100422Pesquisa Veterinária Brasileira v.41 2021reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-6999info:eu-repo/semantics/openAccessFreitas,Natália P.P.Maior,Maria Márcia M.S.Silva,Beatriz A.P.Bezerra,Marcus R.L.Nunes,José F.G. Neto,SaulTavares,Kaio C.S.Martins,Leonardo T.eng2021-11-22T00:00:00Zoai:scielo:S0100-736X2021000100422Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2021-11-22T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use |
title |
In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use |
spellingShingle |
In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use Freitas,Natália P.P. In-vitro production lyophilization canine platelet-rich plasma regenerative medicine healing dogs |
title_short |
In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use |
title_full |
In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use |
title_fullStr |
In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use |
title_full_unstemmed |
In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use |
title_sort |
In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use |
author |
Freitas,Natália P.P. |
author_facet |
Freitas,Natália P.P. Maior,Maria Márcia M.S. Silva,Beatriz A.P. Bezerra,Marcus R.L. Nunes,José F. G. Neto,Saul Tavares,Kaio C.S. Martins,Leonardo T. |
author_role |
author |
author2 |
Maior,Maria Márcia M.S. Silva,Beatriz A.P. Bezerra,Marcus R.L. Nunes,José F. G. Neto,Saul Tavares,Kaio C.S. Martins,Leonardo T. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Freitas,Natália P.P. Maior,Maria Márcia M.S. Silva,Beatriz A.P. Bezerra,Marcus R.L. Nunes,José F. G. Neto,Saul Tavares,Kaio C.S. Martins,Leonardo T. |
dc.subject.por.fl_str_mv |
In-vitro production lyophilization canine platelet-rich plasma regenerative medicine healing dogs |
topic |
In-vitro production lyophilization canine platelet-rich plasma regenerative medicine healing dogs |
description |
ABSTRACT: Platelet-rich plasma (PRP) has been considered a promising therapeutic alternative, since platelets are rich in growth factors that are used in the Regenerative Medicine field. However, fresh PRP cannot be stored for long periods. This study aimed to develop a protocol for obtaining lyophilized canine PRP capable of maintaining viability after its reconstitution. For that purpose, canine PRP extraction and lyophilization protocols were initially tested. Subsequently, assays were carried out to quantify the growth factors VEGF and TGF-β, before and after the lyophilization process, gelation test and the three-dimensional gel structure analysis of the reconstituted lyophilized PRP by electron microscopy, as well as in vitro cell proliferation test in lyophilized PRP gel. Additionally, the immunogenicity test was performed, using allogeneic samples of lyophilized PRP. The results showed that the lyophilized PRP had adequate therapeutic concentrations of growth factors VEGF and TGF-β (9.1pg/mL and 6161.6pg/mL, respectively). The reconstituted PRP gel after lyophilization showed an in vitro durability of 10 days. Its electron microscopy structure was similar to that of fresh PRP. In the cell proliferation test, an intense division process was verified in mesenchymal stem cells (MSCs) through the three-dimensional mesh structure of the lyophilized PRP gel. The immunogenicity test showed no evidence of an immune reaction. The findings were promising, suggesting the possibility of having a lyophilized canine PRP that can be marketed. New in vivo and in vitro studies must be carried out for therapeutic confirmation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100422 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100422 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-5150-pvb-6999 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.41 2021 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122241216348160 |