Pathological features of cloned calves that died in the neonatal period

Detalhes bibliográficos
Autor(a) principal: Pereira,Lídia dos Santos
Data de Publicação: 2020
Outros Autores: Porto,Mirna R., Reis Júnior,Janildo L., Rumpf,Rodolfo, Silva Júnior,Edson R., Soto-Blanco,Benito, Câmara,Antônio Carlos L., Borges,José Renato J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2020001100852
Resumo: ABSTRACT: Somatic-cell nuclear transfer is a cloning technique that enables the creation of a viable embryo from a donor adult to produce a genetically identical individual. This technique opens numerous potential possibilities for medicine and animal reproduction. However, several reports have documented cloning-related issues. Embryo and fetal losses remain significantly higher than in other techniques, and there is a high incidence of dystocia and hydrops, which decreases efficiency and increases costs. Animals delivered at term often exhibit a syndrome known as macrosomia and experience difficulties in adapting to life outside the uterus, and death is a common outcome. In the present study, 41 cloned calves that died in the neonatal period were subjected to gross and histopathological examination. Most important gross lesions were found in the liver (enlargement, congestion, yellowish color), kidneys (brownish color at surface and cut, and cysts), lungs (atelectasis, parenchymal consolidation, and secretions in bronchi and bronchioles), and heart (concentric and eccentric hypertrophy, hematic cysts, persistence of ductus arteriosus). Primary microscopic findings were seen in the liver, kidneys, and lungs from neonatal calves. In the liver, 85% of the animals exhibited hepatic degeneration. The presence of a brownish pigment within the cortical tubules of the kidneys was found in approximately 90% of the samples; the presence of this pigment has not been previously reported in cloned calves. In the lungs, a large number of animals exhibiting lesions characteristic of pneumonia (55%). These changes were the pivotal causes of death, mainly due to problems in adapting to life outside the uterus and opportunistic infections in the neonatal period. Further investigation focusing on pathological anatomical changes is necessary to map these abnormalities in cloned animals.
id EMBRAPA-2_2aba1c45c118310921fd13bb0a0e7899
oai_identifier_str oai:scielo:S0100-736X2020001100852
network_acronym_str EMBRAPA-2
network_name_str Pesquisa Veterinária Brasileira (Online)
repository_id_str
spelling Pathological features of cloned calves that died in the neonatal periodNeonatologycattlecalfclonenecropsyhistopathologyABSTRACT: Somatic-cell nuclear transfer is a cloning technique that enables the creation of a viable embryo from a donor adult to produce a genetically identical individual. This technique opens numerous potential possibilities for medicine and animal reproduction. However, several reports have documented cloning-related issues. Embryo and fetal losses remain significantly higher than in other techniques, and there is a high incidence of dystocia and hydrops, which decreases efficiency and increases costs. Animals delivered at term often exhibit a syndrome known as macrosomia and experience difficulties in adapting to life outside the uterus, and death is a common outcome. In the present study, 41 cloned calves that died in the neonatal period were subjected to gross and histopathological examination. Most important gross lesions were found in the liver (enlargement, congestion, yellowish color), kidneys (brownish color at surface and cut, and cysts), lungs (atelectasis, parenchymal consolidation, and secretions in bronchi and bronchioles), and heart (concentric and eccentric hypertrophy, hematic cysts, persistence of ductus arteriosus). Primary microscopic findings were seen in the liver, kidneys, and lungs from neonatal calves. In the liver, 85% of the animals exhibited hepatic degeneration. The presence of a brownish pigment within the cortical tubules of the kidneys was found in approximately 90% of the samples; the presence of this pigment has not been previously reported in cloned calves. In the lungs, a large number of animals exhibiting lesions characteristic of pneumonia (55%). These changes were the pivotal causes of death, mainly due to problems in adapting to life outside the uterus and opportunistic infections in the neonatal period. Further investigation focusing on pathological anatomical changes is necessary to map these abnormalities in cloned animals.Colégio Brasileiro de Patologia Animal - CBPA2020-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2020001100852Pesquisa Veterinária Brasileira v.40 n.11 2020reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-6720info:eu-repo/semantics/openAccessPereira,Lídia dos SantosPorto,Mirna R.Reis Júnior,Janildo L.Rumpf,RodolfoSilva Júnior,Edson R.Soto-Blanco,BenitoCâmara,Antônio Carlos L.Borges,José Renato J.eng2021-01-15T00:00:00Zoai:scielo:S0100-736X2020001100852Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2021-01-15T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Pathological features of cloned calves that died in the neonatal period
title Pathological features of cloned calves that died in the neonatal period
spellingShingle Pathological features of cloned calves that died in the neonatal period
Pereira,Lídia dos Santos
Neonatology
cattle
calf
clone
necropsy
histopathology
title_short Pathological features of cloned calves that died in the neonatal period
title_full Pathological features of cloned calves that died in the neonatal period
title_fullStr Pathological features of cloned calves that died in the neonatal period
title_full_unstemmed Pathological features of cloned calves that died in the neonatal period
title_sort Pathological features of cloned calves that died in the neonatal period
author Pereira,Lídia dos Santos
author_facet Pereira,Lídia dos Santos
Porto,Mirna R.
Reis Júnior,Janildo L.
Rumpf,Rodolfo
Silva Júnior,Edson R.
Soto-Blanco,Benito
Câmara,Antônio Carlos L.
Borges,José Renato J.
author_role author
author2 Porto,Mirna R.
Reis Júnior,Janildo L.
Rumpf,Rodolfo
Silva Júnior,Edson R.
Soto-Blanco,Benito
Câmara,Antônio Carlos L.
Borges,José Renato J.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira,Lídia dos Santos
Porto,Mirna R.
Reis Júnior,Janildo L.
Rumpf,Rodolfo
Silva Júnior,Edson R.
Soto-Blanco,Benito
Câmara,Antônio Carlos L.
Borges,José Renato J.
dc.subject.por.fl_str_mv Neonatology
cattle
calf
clone
necropsy
histopathology
topic Neonatology
cattle
calf
clone
necropsy
histopathology
description ABSTRACT: Somatic-cell nuclear transfer is a cloning technique that enables the creation of a viable embryo from a donor adult to produce a genetically identical individual. This technique opens numerous potential possibilities for medicine and animal reproduction. However, several reports have documented cloning-related issues. Embryo and fetal losses remain significantly higher than in other techniques, and there is a high incidence of dystocia and hydrops, which decreases efficiency and increases costs. Animals delivered at term often exhibit a syndrome known as macrosomia and experience difficulties in adapting to life outside the uterus, and death is a common outcome. In the present study, 41 cloned calves that died in the neonatal period were subjected to gross and histopathological examination. Most important gross lesions were found in the liver (enlargement, congestion, yellowish color), kidneys (brownish color at surface and cut, and cysts), lungs (atelectasis, parenchymal consolidation, and secretions in bronchi and bronchioles), and heart (concentric and eccentric hypertrophy, hematic cysts, persistence of ductus arteriosus). Primary microscopic findings were seen in the liver, kidneys, and lungs from neonatal calves. In the liver, 85% of the animals exhibited hepatic degeneration. The presence of a brownish pigment within the cortical tubules of the kidneys was found in approximately 90% of the samples; the presence of this pigment has not been previously reported in cloned calves. In the lungs, a large number of animals exhibiting lesions characteristic of pneumonia (55%). These changes were the pivotal causes of death, mainly due to problems in adapting to life outside the uterus and opportunistic infections in the neonatal period. Further investigation focusing on pathological anatomical changes is necessary to map these abnormalities in cloned animals.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2020001100852
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2020001100852
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-5150-pvb-6720
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.40 n.11 2020
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
_version_ 1754122240768606208