Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs

Detalhes bibliográficos
Autor(a) principal: Minho,Alessandro Pelegrine
Data de Publicação: 2004
Outros Autores: Freire,Roberta Lemos, Vidotto,Odilon, Gennari,Solange Maria, Marana,Elizabete Marangoni, Garcia,João Luis, Navarro,Italmar Teodorico
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2004000400005
Resumo: The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.
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spelling Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigsToxoplasma gondiiswineantibodiesserologyindirect fluorescent antibody test (IFAT)modified agglutination test (MAT)The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.Colégio Brasileiro de Patologia Animal - CBPA2004-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2004000400005Pesquisa Veterinária Brasileira v.24 n.4 2004reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2004000400005info:eu-repo/semantics/openAccessMinho,Alessandro PelegrineFreire,Roberta LemosVidotto,OdilonGennari,Solange MariaMarana,Elizabete MarangoniGarcia,João LuisNavarro,Italmar Teodoricoeng2005-03-21T00:00:00Zoai:scielo:S0100-736X2004000400005Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2005-03-21T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
title Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
spellingShingle Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
Minho,Alessandro Pelegrine
Toxoplasma gondii
swine
antibodies
serology
indirect fluorescent antibody test (IFAT)
modified agglutination test (MAT)
title_short Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
title_full Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
title_fullStr Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
title_full_unstemmed Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
title_sort Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
author Minho,Alessandro Pelegrine
author_facet Minho,Alessandro Pelegrine
Freire,Roberta Lemos
Vidotto,Odilon
Gennari,Solange Maria
Marana,Elizabete Marangoni
Garcia,João Luis
Navarro,Italmar Teodorico
author_role author
author2 Freire,Roberta Lemos
Vidotto,Odilon
Gennari,Solange Maria
Marana,Elizabete Marangoni
Garcia,João Luis
Navarro,Italmar Teodorico
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Minho,Alessandro Pelegrine
Freire,Roberta Lemos
Vidotto,Odilon
Gennari,Solange Maria
Marana,Elizabete Marangoni
Garcia,João Luis
Navarro,Italmar Teodorico
dc.subject.por.fl_str_mv Toxoplasma gondii
swine
antibodies
serology
indirect fluorescent antibody test (IFAT)
modified agglutination test (MAT)
topic Toxoplasma gondii
swine
antibodies
serology
indirect fluorescent antibody test (IFAT)
modified agglutination test (MAT)
description The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.
publishDate 2004
dc.date.none.fl_str_mv 2004-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2004000400005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2004000400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2004000400005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.24 n.4 2004
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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