Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000200320 |
Resumo: | ABSTRACT: Some studies have shown the role played by matrix metalloproteinases and their inhibitors in doxorubicin cardiotoxicity. In this study, we sought to investigate how plasma and myocardial MMP 2 and 9 perform in rabbits with doxorubicin-induced cardiomyopathy, searching for a correlation between the activity of these collagenases and cardiac remodeling. Cardiomyopathy was induced by doxorubicin given intravenously twice a week for six consecutive weeks. Plasma MMP activity and the echocardiogram were assessed at baseline, and at 15 and 45 days after first injection of doxorubicin. The myocardial activity of these enzymes was solely evaluated in nine rabbits at 45 days, and results were compared with nine healthy controls. We only identified the full-length forms of both MMP 2 and 9 throughout the study. The plasma pro-MMP 2 reduced along the deterioration of cardiac function, while the pro-MMP 9 increased significantly at T45 as compared to baseline and T15. A negative significant correlation was found to exist between the plasma activity of pro-MMP 2 and mitral E-to-mitral septal annular early diastolic velocity ratio, which is an estimate of mean left atrial pressure and congestion. Only pro-MMP 2 was found in myocardial samples, and mean activity of such enzyme was statistically lower than that recorded for healthy controls. Although no active form was documented for either collagenase, the duration of the treatment with doxorubicin played a role in the alteration of plasma pro-forms activity. However, these changes could not be associated with most echocardiographic parameters that are supportive of cardiac remodeling. |
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Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathyMatrix metalloproteinasesrabbitsdoxorubicincardiomyopathyzymographyenzyme activitycardiac diseaseechocardiogramcollagenaseclinicsABSTRACT: Some studies have shown the role played by matrix metalloproteinases and their inhibitors in doxorubicin cardiotoxicity. In this study, we sought to investigate how plasma and myocardial MMP 2 and 9 perform in rabbits with doxorubicin-induced cardiomyopathy, searching for a correlation between the activity of these collagenases and cardiac remodeling. Cardiomyopathy was induced by doxorubicin given intravenously twice a week for six consecutive weeks. Plasma MMP activity and the echocardiogram were assessed at baseline, and at 15 and 45 days after first injection of doxorubicin. The myocardial activity of these enzymes was solely evaluated in nine rabbits at 45 days, and results were compared with nine healthy controls. We only identified the full-length forms of both MMP 2 and 9 throughout the study. The plasma pro-MMP 2 reduced along the deterioration of cardiac function, while the pro-MMP 9 increased significantly at T45 as compared to baseline and T15. A negative significant correlation was found to exist between the plasma activity of pro-MMP 2 and mitral E-to-mitral septal annular early diastolic velocity ratio, which is an estimate of mean left atrial pressure and congestion. Only pro-MMP 2 was found in myocardial samples, and mean activity of such enzyme was statistically lower than that recorded for healthy controls. Although no active form was documented for either collagenase, the duration of the treatment with doxorubicin played a role in the alteration of plasma pro-forms activity. However, these changes could not be associated with most echocardiographic parameters that are supportive of cardiac remodeling.Colégio Brasileiro de Patologia Animal - CBPA2018-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000200320Pesquisa Veterinária Brasileira v.38 n.2 2018reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-4990info:eu-repo/semantics/openAccessNogueira,Sheila S.S.Sousa,Marlos G.Gava,Fabio N.Rosa,Fernando A.Melo,Guilherme D.Dittrich,GustavoMachado,Gisele F.Camacho,Aparecido A.eng2018-04-19T00:00:00Zoai:scielo:S0100-736X2018000200320Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2018-04-19T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy |
title |
Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy |
spellingShingle |
Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy Nogueira,Sheila S.S. Matrix metalloproteinases rabbits doxorubicin cardiomyopathy zymography enzyme activity cardiac disease echocardiogram collagenase clinics |
title_short |
Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy |
title_full |
Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy |
title_fullStr |
Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy |
title_full_unstemmed |
Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy |
title_sort |
Matrix metalloproteinases 2 and 9 in rabbits with doxorubicin-induced cardiomyopathy |
author |
Nogueira,Sheila S.S. |
author_facet |
Nogueira,Sheila S.S. Sousa,Marlos G. Gava,Fabio N. Rosa,Fernando A. Melo,Guilherme D. Dittrich,Gustavo Machado,Gisele F. Camacho,Aparecido A. |
author_role |
author |
author2 |
Sousa,Marlos G. Gava,Fabio N. Rosa,Fernando A. Melo,Guilherme D. Dittrich,Gustavo Machado,Gisele F. Camacho,Aparecido A. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Nogueira,Sheila S.S. Sousa,Marlos G. Gava,Fabio N. Rosa,Fernando A. Melo,Guilherme D. Dittrich,Gustavo Machado,Gisele F. Camacho,Aparecido A. |
dc.subject.por.fl_str_mv |
Matrix metalloproteinases rabbits doxorubicin cardiomyopathy zymography enzyme activity cardiac disease echocardiogram collagenase clinics |
topic |
Matrix metalloproteinases rabbits doxorubicin cardiomyopathy zymography enzyme activity cardiac disease echocardiogram collagenase clinics |
description |
ABSTRACT: Some studies have shown the role played by matrix metalloproteinases and their inhibitors in doxorubicin cardiotoxicity. In this study, we sought to investigate how plasma and myocardial MMP 2 and 9 perform in rabbits with doxorubicin-induced cardiomyopathy, searching for a correlation between the activity of these collagenases and cardiac remodeling. Cardiomyopathy was induced by doxorubicin given intravenously twice a week for six consecutive weeks. Plasma MMP activity and the echocardiogram were assessed at baseline, and at 15 and 45 days after first injection of doxorubicin. The myocardial activity of these enzymes was solely evaluated in nine rabbits at 45 days, and results were compared with nine healthy controls. We only identified the full-length forms of both MMP 2 and 9 throughout the study. The plasma pro-MMP 2 reduced along the deterioration of cardiac function, while the pro-MMP 9 increased significantly at T45 as compared to baseline and T15. A negative significant correlation was found to exist between the plasma activity of pro-MMP 2 and mitral E-to-mitral septal annular early diastolic velocity ratio, which is an estimate of mean left atrial pressure and congestion. Only pro-MMP 2 was found in myocardial samples, and mean activity of such enzyme was statistically lower than that recorded for healthy controls. Although no active form was documented for either collagenase, the duration of the treatment with doxorubicin played a role in the alteration of plasma pro-forms activity. However, these changes could not be associated with most echocardiographic parameters that are supportive of cardiac remodeling. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000200320 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000200320 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-5150-pvb-4990 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.38 n.2 2018 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
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1754122237987782656 |