Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001000939 |
Resumo: | ABSTRACT: Equine neorickettsiosis (EN), also known as Potomac Horse Fever, is a non-contagious disease caused by the bacterium Neorickettsia risticii of the Anaplasmataceae family. The objectives of this study were to detect the presence of anti-N. risticii antibodies by the indirect immunofluorescence assay (IFA) and of its DNA by qPCR in equids at high and low altitude regions in the State of Rio de Janeiro, Brazil, and to identify factors associated with seropositive equids by multiple logistic regression analysis. The frequency of anti-N. risticii antibodies was 16.05% (n=113/704). The animal age and breeding region were the factors that influenced the seropositivity rate for N. risticii in the equids (p<0.05). Equids from the lowland region had higher seropositivity (p<0.05; OR=5.87) compared to those of the mountain region. The presence of snails on the farm was a factor associated with this result (p<0.05; OR=2.88). In the lowland region, age of the animal and site of breeding were protective factors for the detection of antibodies anti-N. risticii in equids, with lower frequency of seropositivity in younger animals (p<0.05; OR=0.06) and in animals raised in dry areas (p<0.05; OR=0.22). The presence of the target DNA of N. risticii by qPCR was not observed in any of the samples tested. The existence of seropositive equids for N. risticii demonstrates a possible circulation of this agent in the studied area, and that the age related characteristics and equids breeding region are important factors regarding seropositivity in the State of Rio de Janeiro. |
id |
EMBRAPA-2_42ba5444398359908b6f721a9dbdc8b7 |
---|---|
oai_identifier_str |
oai:scielo:S0100-736X2016001000939 |
network_acronym_str |
EMBRAPA-2 |
network_name_str |
Pesquisa Veterinária Brasileira (Online) |
repository_id_str |
|
spelling |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, BrazilNeorickettsia risticiiequidsRio de Janeiroequine monocytic ehrlichiosisserological diagnosisepidemiologyABSTRACT: Equine neorickettsiosis (EN), also known as Potomac Horse Fever, is a non-contagious disease caused by the bacterium Neorickettsia risticii of the Anaplasmataceae family. The objectives of this study were to detect the presence of anti-N. risticii antibodies by the indirect immunofluorescence assay (IFA) and of its DNA by qPCR in equids at high and low altitude regions in the State of Rio de Janeiro, Brazil, and to identify factors associated with seropositive equids by multiple logistic regression analysis. The frequency of anti-N. risticii antibodies was 16.05% (n=113/704). The animal age and breeding region were the factors that influenced the seropositivity rate for N. risticii in the equids (p<0.05). Equids from the lowland region had higher seropositivity (p<0.05; OR=5.87) compared to those of the mountain region. The presence of snails on the farm was a factor associated with this result (p<0.05; OR=2.88). In the lowland region, age of the animal and site of breeding were protective factors for the detection of antibodies anti-N. risticii in equids, with lower frequency of seropositivity in younger animals (p<0.05; OR=0.06) and in animals raised in dry areas (p<0.05; OR=0.22). The presence of the target DNA of N. risticii by qPCR was not observed in any of the samples tested. The existence of seropositive equids for N. risticii demonstrates a possible circulation of this agent in the studied area, and that the age related characteristics and equids breeding region are important factors regarding seropositivity in the State of Rio de Janeiro.Colégio Brasileiro de Patologia Animal - CBPA2016-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001000939Pesquisa Veterinária Brasileira v.36 n.10 2016reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/s0100-736x2016001000004info:eu-repo/semantics/openAccessRoier,Erica C.R.Costa,Renata L.Pires,Marcus S.Vilela,Joice A.R.Santos,Tiago M. dosSantos,Huarrisson A.Baldani,Cristiane D.Massard,Carlos L.eng2017-01-17T00:00:00Zoai:scielo:S0100-736X2016001000939Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2017-01-17T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
spellingShingle |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil Roier,Erica C.R. Neorickettsia risticii equids Rio de Janeiro equine monocytic ehrlichiosis serological diagnosis epidemiology |
title_short |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title_full |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title_fullStr |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title_full_unstemmed |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
title_sort |
Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil |
author |
Roier,Erica C.R. |
author_facet |
Roier,Erica C.R. Costa,Renata L. Pires,Marcus S. Vilela,Joice A.R. Santos,Tiago M. dos Santos,Huarrisson A. Baldani,Cristiane D. Massard,Carlos L. |
author_role |
author |
author2 |
Costa,Renata L. Pires,Marcus S. Vilela,Joice A.R. Santos,Tiago M. dos Santos,Huarrisson A. Baldani,Cristiane D. Massard,Carlos L. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Roier,Erica C.R. Costa,Renata L. Pires,Marcus S. Vilela,Joice A.R. Santos,Tiago M. dos Santos,Huarrisson A. Baldani,Cristiane D. Massard,Carlos L. |
dc.subject.por.fl_str_mv |
Neorickettsia risticii equids Rio de Janeiro equine monocytic ehrlichiosis serological diagnosis epidemiology |
topic |
Neorickettsia risticii equids Rio de Janeiro equine monocytic ehrlichiosis serological diagnosis epidemiology |
description |
ABSTRACT: Equine neorickettsiosis (EN), also known as Potomac Horse Fever, is a non-contagious disease caused by the bacterium Neorickettsia risticii of the Anaplasmataceae family. The objectives of this study were to detect the presence of anti-N. risticii antibodies by the indirect immunofluorescence assay (IFA) and of its DNA by qPCR in equids at high and low altitude regions in the State of Rio de Janeiro, Brazil, and to identify factors associated with seropositive equids by multiple logistic regression analysis. The frequency of anti-N. risticii antibodies was 16.05% (n=113/704). The animal age and breeding region were the factors that influenced the seropositivity rate for N. risticii in the equids (p<0.05). Equids from the lowland region had higher seropositivity (p<0.05; OR=5.87) compared to those of the mountain region. The presence of snails on the farm was a factor associated with this result (p<0.05; OR=2.88). In the lowland region, age of the animal and site of breeding were protective factors for the detection of antibodies anti-N. risticii in equids, with lower frequency of seropositivity in younger animals (p<0.05; OR=0.06) and in animals raised in dry areas (p<0.05; OR=0.22). The presence of the target DNA of N. risticii by qPCR was not observed in any of the samples tested. The existence of seropositive equids for N. risticii demonstrates a possible circulation of this agent in the studied area, and that the age related characteristics and equids breeding region are important factors regarding seropositivity in the State of Rio de Janeiro. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001000939 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016001000939 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0100-736x2016001000004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.36 n.10 2016 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122236443230208 |