Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000900710 |
Resumo: | ABSTRACT: The objective of this study was to evaluate the methods of collecting Brachiaria spp. (Simulated Grazing vs. Total Collection) for the determination of protodioscin concentrations and to monitor the intoxication in sheep susceptible and resistant to poisoning by Brachiariaspp. The collection of Brachiaria spp. was done through two different methods. The first one consisted in throwing a metallic square at 10 random picket points. The second was performed simulating, with the hands, the grazing behavior of sheep. The leaf blades obtained by the two methods were conditioned in a forced ventilation oven. The extraction and quantification of protodioscin from the dry milled plant material was done by reverse-phase high-performance liquid chromatography (HPLC). The collections were carried out at three stages of the life of experimental lamb (suckling, rearing, and finishing). The lambs were divided into resistant and susceptible to intoxication by Brachiaria spp. The criterion for a lamb to be considered sensitive was to present some clinical signs at some stage of its life. No significant differences were observed between the general averages of the two collections when the evaluation was done in the whole period. However, a significant difference was found at the onset of the clinical signs. The concentrations of protodioscin in this period were higher in Brachiaria spp. collected by the simulating grazing method. Both methodologies were efficient to determine the level of protodioscin in the pastures of Brachiaria spp. However, during the periods when the outbreaks occurred, the simulated grazing method was more reliable. |
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Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. PoisoningCollection methodsprotodioscin concentrationsBrachiaria spp.poisoninghepatogenic photosensitizationsimulated grazingtoxic plantssmall ruminantsABSTRACT: The objective of this study was to evaluate the methods of collecting Brachiaria spp. (Simulated Grazing vs. Total Collection) for the determination of protodioscin concentrations and to monitor the intoxication in sheep susceptible and resistant to poisoning by Brachiariaspp. The collection of Brachiaria spp. was done through two different methods. The first one consisted in throwing a metallic square at 10 random picket points. The second was performed simulating, with the hands, the grazing behavior of sheep. The leaf blades obtained by the two methods were conditioned in a forced ventilation oven. The extraction and quantification of protodioscin from the dry milled plant material was done by reverse-phase high-performance liquid chromatography (HPLC). The collections were carried out at three stages of the life of experimental lamb (suckling, rearing, and finishing). The lambs were divided into resistant and susceptible to intoxication by Brachiaria spp. The criterion for a lamb to be considered sensitive was to present some clinical signs at some stage of its life. No significant differences were observed between the general averages of the two collections when the evaluation was done in the whole period. However, a significant difference was found at the onset of the clinical signs. The concentrations of protodioscin in this period were higher in Brachiaria spp. collected by the simulating grazing method. Both methodologies were efficient to determine the level of protodioscin in the pastures of Brachiaria spp. However, during the periods when the outbreaks occurred, the simulated grazing method was more reliable.Colégio Brasileiro de Patologia Animal - CBPA2019-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000900710Pesquisa Veterinária Brasileira v.39 n.9 2019reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-6226info:eu-repo/semantics/openAccessMelo,Gleice K.A.Silva,Westerlly J.Pupin,Rayane C.Heckler,Rubiane F.Nonato,Lucimara M.Ítavo,Camila C.B.F.Ferreira,Valquíria B.N.Lemos,Ricardo A.A.eng2019-11-01T00:00:00Zoai:scielo:S0100-736X2019000900710Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2019-11-01T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning |
title |
Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning |
spellingShingle |
Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning Melo,Gleice K.A. Collection methods protodioscin concentrations Brachiaria spp. poisoning hepatogenic photosensitization simulated grazing toxic plants small ruminants |
title_short |
Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning |
title_full |
Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning |
title_fullStr |
Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning |
title_full_unstemmed |
Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning |
title_sort |
Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning |
author |
Melo,Gleice K.A. |
author_facet |
Melo,Gleice K.A. Silva,Westerlly J. Pupin,Rayane C. Heckler,Rubiane F. Nonato,Lucimara M. Ítavo,Camila C.B.F. Ferreira,Valquíria B.N. Lemos,Ricardo A.A. |
author_role |
author |
author2 |
Silva,Westerlly J. Pupin,Rayane C. Heckler,Rubiane F. Nonato,Lucimara M. Ítavo,Camila C.B.F. Ferreira,Valquíria B.N. Lemos,Ricardo A.A. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Melo,Gleice K.A. Silva,Westerlly J. Pupin,Rayane C. Heckler,Rubiane F. Nonato,Lucimara M. Ítavo,Camila C.B.F. Ferreira,Valquíria B.N. Lemos,Ricardo A.A. |
dc.subject.por.fl_str_mv |
Collection methods protodioscin concentrations Brachiaria spp. poisoning hepatogenic photosensitization simulated grazing toxic plants small ruminants |
topic |
Collection methods protodioscin concentrations Brachiaria spp. poisoning hepatogenic photosensitization simulated grazing toxic plants small ruminants |
description |
ABSTRACT: The objective of this study was to evaluate the methods of collecting Brachiaria spp. (Simulated Grazing vs. Total Collection) for the determination of protodioscin concentrations and to monitor the intoxication in sheep susceptible and resistant to poisoning by Brachiariaspp. The collection of Brachiaria spp. was done through two different methods. The first one consisted in throwing a metallic square at 10 random picket points. The second was performed simulating, with the hands, the grazing behavior of sheep. The leaf blades obtained by the two methods were conditioned in a forced ventilation oven. The extraction and quantification of protodioscin from the dry milled plant material was done by reverse-phase high-performance liquid chromatography (HPLC). The collections were carried out at three stages of the life of experimental lamb (suckling, rearing, and finishing). The lambs were divided into resistant and susceptible to intoxication by Brachiaria spp. The criterion for a lamb to be considered sensitive was to present some clinical signs at some stage of its life. No significant differences were observed between the general averages of the two collections when the evaluation was done in the whole period. However, a significant difference was found at the onset of the clinical signs. The concentrations of protodioscin in this period were higher in Brachiaria spp. collected by the simulating grazing method. Both methodologies were efficient to determine the level of protodioscin in the pastures of Brachiaria spp. However, during the periods when the outbreaks occurred, the simulated grazing method was more reliable. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000900710 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000900710 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-5150-pvb-6226 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.39 n.9 2019 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122239854247936 |