Chorion biopsy in mongrel dogs

Detalhes bibliográficos
Autor(a) principal: Ferreira,Guilherme J.
Data de Publicação: 2009
Outros Autores: Carvalho,Ana F., Branco,Érika, Cabral,Rosa, Gregores,Guilherme B., Fioretto,Emerson T., Lima,Ana Rita de, Sarmento,Carlos Alberto P., Miglino,Maria Angelica
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2009001000003
Resumo: With the great development of the gestational studies in all of the species, we noticed the necessity of adaptations of these techniques for prenatal diagnosis in dogs. Based on this, we studied the feasibility of chorion biopsy guided by ultrasound. Our results demonstrated accuracy on the sex determination being 2 males and 12 females, as well as it would be possible to identify chromosome alteration due to the quality of samplings. Sex determination was accomplished with the identification of Y gene chromosomes in PCR technique. After the collection, fragments were prepared for light microscopy studies and revealed fetal chorion tissue, blood colloid and erythrocyte. In the whole material we found hemosiderin impregnations due to the hemolysis and to the residue of blood of the placental marginal hematomes. The submitted female dogs to this technique demonstrated normal puppy births without death.
id EMBRAPA-2_6076f4dc05635aa0e5fada5dbb7900d5
oai_identifier_str oai:scielo:S0100-736X2009001000003
network_acronym_str EMBRAPA-2
network_name_str Pesquisa Veterinária Brasileira (Online)
repository_id_str
spelling Chorion biopsy in mongrel dogsChorionbiopsyplacentadogWith the great development of the gestational studies in all of the species, we noticed the necessity of adaptations of these techniques for prenatal diagnosis in dogs. Based on this, we studied the feasibility of chorion biopsy guided by ultrasound. Our results demonstrated accuracy on the sex determination being 2 males and 12 females, as well as it would be possible to identify chromosome alteration due to the quality of samplings. Sex determination was accomplished with the identification of Y gene chromosomes in PCR technique. After the collection, fragments were prepared for light microscopy studies and revealed fetal chorion tissue, blood colloid and erythrocyte. In the whole material we found hemosiderin impregnations due to the hemolysis and to the residue of blood of the placental marginal hematomes. The submitted female dogs to this technique demonstrated normal puppy births without death.Colégio Brasileiro de Patologia Animal - CBPA2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2009001000003Pesquisa Veterinária Brasileira v.29 n.10 2009reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2009001000003info:eu-repo/semantics/openAccessFerreira,Guilherme J.Carvalho,Ana F.Branco,ÉrikaCabral,RosaGregores,Guilherme B.Fioretto,Emerson T.Lima,Ana Rita deSarmento,Carlos Alberto P.Miglino,Maria Angelicaeng2010-01-20T00:00:00Zoai:scielo:S0100-736X2009001000003Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2010-01-20T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Chorion biopsy in mongrel dogs
title Chorion biopsy in mongrel dogs
spellingShingle Chorion biopsy in mongrel dogs
Ferreira,Guilherme J.
Chorion
biopsy
placenta
dog
title_short Chorion biopsy in mongrel dogs
title_full Chorion biopsy in mongrel dogs
title_fullStr Chorion biopsy in mongrel dogs
title_full_unstemmed Chorion biopsy in mongrel dogs
title_sort Chorion biopsy in mongrel dogs
author Ferreira,Guilherme J.
author_facet Ferreira,Guilherme J.
Carvalho,Ana F.
Branco,Érika
Cabral,Rosa
Gregores,Guilherme B.
Fioretto,Emerson T.
Lima,Ana Rita de
Sarmento,Carlos Alberto P.
Miglino,Maria Angelica
author_role author
author2 Carvalho,Ana F.
Branco,Érika
Cabral,Rosa
Gregores,Guilherme B.
Fioretto,Emerson T.
Lima,Ana Rita de
Sarmento,Carlos Alberto P.
Miglino,Maria Angelica
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira,Guilherme J.
Carvalho,Ana F.
Branco,Érika
Cabral,Rosa
Gregores,Guilherme B.
Fioretto,Emerson T.
Lima,Ana Rita de
Sarmento,Carlos Alberto P.
Miglino,Maria Angelica
dc.subject.por.fl_str_mv Chorion
biopsy
placenta
dog
topic Chorion
biopsy
placenta
dog
description With the great development of the gestational studies in all of the species, we noticed the necessity of adaptations of these techniques for prenatal diagnosis in dogs. Based on this, we studied the feasibility of chorion biopsy guided by ultrasound. Our results demonstrated accuracy on the sex determination being 2 males and 12 females, as well as it would be possible to identify chromosome alteration due to the quality of samplings. Sex determination was accomplished with the identification of Y gene chromosomes in PCR technique. After the collection, fragments were prepared for light microscopy studies and revealed fetal chorion tissue, blood colloid and erythrocyte. In the whole material we found hemosiderin impregnations due to the hemolysis and to the residue of blood of the placental marginal hematomes. The submitted female dogs to this technique demonstrated normal puppy births without death.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2009001000003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2009001000003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2009001000003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.29 n.10 2009
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
_version_ 1754122229894873088