Anaplasma marginale infection in cattle from south-western Amazonia

• Autor(a) principal: Brito,Luciana Gatto
• Data de Publicação: 2010
• Outros Autores: Oliveira,Márcia Cristina de Sena, Rocha,Rodrigo Barros, Silva Netto,Francelino Goulart da, Marim,Adriana Denise, Souza,Gislaine Cristina Rodrigues de, Vendrame,Fabiano Benitez, Moura,Maria Manuela da Fonseca
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Pesquisa Veterinária Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010000300011
• topic: Anaplasma marginale; epidemiology; cattle; south-western Amazonia; Brazil

The present study provides the first epidemiological data regarding infection by Anaplasma marginale in cattle reared in south-western Brazilian Amazonia. One simple procedure was adapted for the extraction of DNA from blood clots collected in seven microregions of Rondônia State and two mesoregions of Acre State. PCR method was used to asses the frequency of A. marginale infections in 4 to12-month-old cattle. The cattle infection was investigated by polymerase chain reaction (PCR) using the specific primer "msp5" for A. marginale. The DNA amplifications revealed that the mean frequency of A. marginale infection was 98.6% (1,627/1,650) in samples from Rondonia, and 92.87% (208/225) in samples from Acre. The high frequency of A. marginale infections in 4 to 12-month-old cattle indicate a situation of enzootic stability in the studied areas and are comparable to those detected by immunodiagnosis in different endemic regions in Brazil. The DNA extraction of clotted blood method described here can be used for epidemiological studies on anaplasmosis and other bovine hemoparasites.