Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000500967 |
Resumo: | ABSTRACT: Nerium oleander is an ornamental cardiotoxic plant found in tropical and subtropical areas of the World. Its toxicity is related to the content of cardioactive glycosides, mainly oleandrin, found throughout the plant. The present study aimed to describe a new and improved method for oleandrin detection in tissue samples. The determination of oleandrin was made after extraction with a modified QuEChERS technique and measurement by UFLC-MS/MS. A total of 36 guinea pigs (Cavia porcellus) were distributed into 3 groups (n=12): control group that received only water orally (CON), and two treated groups that received hydroalcoholic oleander extract at doses of 150mg.kg-1 (OLE 150) and 300mg.kg-1 (OLE 300) in single oral dose. After three hours, fragments of heart, kidneys, liver and brain were collected for determination of oleandrin levels. The extraction and chromatographic procedures were effective for oleandrin detection and quantification in tissues, with retention time of 1.2 min and detection limit of 0.001μg g-1. The chromatographic analysis of treated guinea pigs indicated that oleandrin is distributed equally among the analyzed tissues. The developed methodology is a reliable, effective and rapid form of diagnosis of N. oleander poisoning based on necropsy tissue samples. |
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Improved method for diagnosis of Nerium oleander poisoning in necropsy tissuesOleanderNerium oleandercardenolidesdiagnostic testingliquid chromatographymass spectrometryguinea pigstoxicosesABSTRACT: Nerium oleander is an ornamental cardiotoxic plant found in tropical and subtropical areas of the World. Its toxicity is related to the content of cardioactive glycosides, mainly oleandrin, found throughout the plant. The present study aimed to describe a new and improved method for oleandrin detection in tissue samples. The determination of oleandrin was made after extraction with a modified QuEChERS technique and measurement by UFLC-MS/MS. A total of 36 guinea pigs (Cavia porcellus) were distributed into 3 groups (n=12): control group that received only water orally (CON), and two treated groups that received hydroalcoholic oleander extract at doses of 150mg.kg-1 (OLE 150) and 300mg.kg-1 (OLE 300) in single oral dose. After three hours, fragments of heart, kidneys, liver and brain were collected for determination of oleandrin levels. The extraction and chromatographic procedures were effective for oleandrin detection and quantification in tissues, with retention time of 1.2 min and detection limit of 0.001μg g-1. The chromatographic analysis of treated guinea pigs indicated that oleandrin is distributed equally among the analyzed tissues. The developed methodology is a reliable, effective and rapid form of diagnosis of N. oleander poisoning based on necropsy tissue samples.Colégio Brasileiro de Patologia Animal - CBPA2018-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000500967Pesquisa Veterinária Brasileira v.38 n.5 2018reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-5285info:eu-repo/semantics/openAccessBotelho,Ana F.M.Oliveira,Fabiano A.S.Fiúza,Aparecida T.L.Pedroza,Heloísa P.Branco,Stephanie E.M.T.Pierezan,FelipeMelo,Marília M.Soto-Blanco,Benitoeng2018-07-26T00:00:00Zoai:scielo:S0100-736X2018000500967Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2018-07-26T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues |
title |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues |
spellingShingle |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues Botelho,Ana F.M. Oleander Nerium oleander cardenolides diagnostic testing liquid chromatography mass spectrometry guinea pigs toxicoses |
title_short |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues |
title_full |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues |
title_fullStr |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues |
title_full_unstemmed |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues |
title_sort |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues |
author |
Botelho,Ana F.M. |
author_facet |
Botelho,Ana F.M. Oliveira,Fabiano A.S. Fiúza,Aparecida T.L. Pedroza,Heloísa P. Branco,Stephanie E.M.T. Pierezan,Felipe Melo,Marília M. Soto-Blanco,Benito |
author_role |
author |
author2 |
Oliveira,Fabiano A.S. Fiúza,Aparecida T.L. Pedroza,Heloísa P. Branco,Stephanie E.M.T. Pierezan,Felipe Melo,Marília M. Soto-Blanco,Benito |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Botelho,Ana F.M. Oliveira,Fabiano A.S. Fiúza,Aparecida T.L. Pedroza,Heloísa P. Branco,Stephanie E.M.T. Pierezan,Felipe Melo,Marília M. Soto-Blanco,Benito |
dc.subject.por.fl_str_mv |
Oleander Nerium oleander cardenolides diagnostic testing liquid chromatography mass spectrometry guinea pigs toxicoses |
topic |
Oleander Nerium oleander cardenolides diagnostic testing liquid chromatography mass spectrometry guinea pigs toxicoses |
description |
ABSTRACT: Nerium oleander is an ornamental cardiotoxic plant found in tropical and subtropical areas of the World. Its toxicity is related to the content of cardioactive glycosides, mainly oleandrin, found throughout the plant. The present study aimed to describe a new and improved method for oleandrin detection in tissue samples. The determination of oleandrin was made after extraction with a modified QuEChERS technique and measurement by UFLC-MS/MS. A total of 36 guinea pigs (Cavia porcellus) were distributed into 3 groups (n=12): control group that received only water orally (CON), and two treated groups that received hydroalcoholic oleander extract at doses of 150mg.kg-1 (OLE 150) and 300mg.kg-1 (OLE 300) in single oral dose. After three hours, fragments of heart, kidneys, liver and brain were collected for determination of oleandrin levels. The extraction and chromatographic procedures were effective for oleandrin detection and quantification in tissues, with retention time of 1.2 min and detection limit of 0.001μg g-1. The chromatographic analysis of treated guinea pigs indicated that oleandrin is distributed equally among the analyzed tissues. The developed methodology is a reliable, effective and rapid form of diagnosis of N. oleander poisoning based on necropsy tissue samples. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000500967 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000500967 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-5150-pvb-5285 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.38 n.5 2018 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122238456496128 |