First phylogenetic analysis of Avipoxvirus (APV) in Brazil

Detalhes bibliográficos
Autor(a) principal: Kunert-Filho,Hiran C.
Data de Publicação: 2016
Outros Autores: Cibulski,Samuel P., Finkler,Fabrine, Grassotti,Tiela T., Jaenisch,Fátima R.F., Brito,Kelly C.T. de, Carvalho,Daiane, Lovato,Maristela, Brito,Benito G. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000500357
Resumo: Abstract: This study represents the first phylogenetic analysis of avian poxvirus recovered from turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in a turkey housing system. The birds displaying characteristic pox lesions which were observed on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly chosen, euthanized and necropsied. Tissues samples were submitted for histopathological analysis and total DNA was further extracted, amplified by conventional PCR, sequenced and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b core protein gene sequence. The histological analysis revealed dermal inflammatory process, granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence identity among the samples, and the sequences were deposited in GenBank®. The four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus isolation, PCR and sequencing includinga large number of specimens from the Brazilian turkey production must be conducted due to the hazardous risk that poxvirus infections may cause to the Brazilian poultry production scenario, given that Brazil's turkey production attracts attention due to its economic importance worldwide. Our findings point to the need to identify the prevalence of APV in Brazilian turkey production, to perform risk assessment studies and continued surveillance of APV infections in both wild and commercial avian species.
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spelling First phylogenetic analysis of Avipoxvirus (APV) in BrazilTurkeyPoxviridaeAvipoxvirusAPVPCRphylogenetic analysis.Abstract: This study represents the first phylogenetic analysis of avian poxvirus recovered from turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in a turkey housing system. The birds displaying characteristic pox lesions which were observed on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly chosen, euthanized and necropsied. Tissues samples were submitted for histopathological analysis and total DNA was further extracted, amplified by conventional PCR, sequenced and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b core protein gene sequence. The histological analysis revealed dermal inflammatory process, granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence identity among the samples, and the sequences were deposited in GenBank®. The four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus isolation, PCR and sequencing includinga large number of specimens from the Brazilian turkey production must be conducted due to the hazardous risk that poxvirus infections may cause to the Brazilian poultry production scenario, given that Brazil's turkey production attracts attention due to its economic importance worldwide. Our findings point to the need to identify the prevalence of APV in Brazilian turkey production, to perform risk assessment studies and continued surveillance of APV infections in both wild and commercial avian species.Colégio Brasileiro de Patologia Animal - CBPA2016-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000500357Pesquisa Veterinária Brasileira v.36 n.5 2016reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2016000500001info:eu-repo/semantics/openAccessKunert-Filho,Hiran C.Cibulski,Samuel P.Finkler,FabrineGrassotti,Tiela T.Jaenisch,Fátima R.F.Brito,Kelly C.T. deCarvalho,DaianeLovato,MaristelaBrito,Benito G. deeng2016-07-25T00:00:00Zoai:scielo:S0100-736X2016000500357Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2016-07-25T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv First phylogenetic analysis of Avipoxvirus (APV) in Brazil
title First phylogenetic analysis of Avipoxvirus (APV) in Brazil
spellingShingle First phylogenetic analysis of Avipoxvirus (APV) in Brazil
Kunert-Filho,Hiran C.
Turkey
Poxviridae
Avipoxvirus
APV
PCR
phylogenetic analysis.
title_short First phylogenetic analysis of Avipoxvirus (APV) in Brazil
title_full First phylogenetic analysis of Avipoxvirus (APV) in Brazil
title_fullStr First phylogenetic analysis of Avipoxvirus (APV) in Brazil
title_full_unstemmed First phylogenetic analysis of Avipoxvirus (APV) in Brazil
title_sort First phylogenetic analysis of Avipoxvirus (APV) in Brazil
author Kunert-Filho,Hiran C.
author_facet Kunert-Filho,Hiran C.
Cibulski,Samuel P.
Finkler,Fabrine
Grassotti,Tiela T.
Jaenisch,Fátima R.F.
Brito,Kelly C.T. de
Carvalho,Daiane
Lovato,Maristela
Brito,Benito G. de
author_role author
author2 Cibulski,Samuel P.
Finkler,Fabrine
Grassotti,Tiela T.
Jaenisch,Fátima R.F.
Brito,Kelly C.T. de
Carvalho,Daiane
Lovato,Maristela
Brito,Benito G. de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kunert-Filho,Hiran C.
Cibulski,Samuel P.
Finkler,Fabrine
Grassotti,Tiela T.
Jaenisch,Fátima R.F.
Brito,Kelly C.T. de
Carvalho,Daiane
Lovato,Maristela
Brito,Benito G. de
dc.subject.por.fl_str_mv Turkey
Poxviridae
Avipoxvirus
APV
PCR
phylogenetic analysis.
topic Turkey
Poxviridae
Avipoxvirus
APV
PCR
phylogenetic analysis.
description Abstract: This study represents the first phylogenetic analysis of avian poxvirus recovered from turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in a turkey housing system. The birds displaying characteristic pox lesions which were observed on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly chosen, euthanized and necropsied. Tissues samples were submitted for histopathological analysis and total DNA was further extracted, amplified by conventional PCR, sequenced and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b core protein gene sequence. The histological analysis revealed dermal inflammatory process, granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence identity among the samples, and the sequences were deposited in GenBank®. The four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus isolation, PCR and sequencing includinga large number of specimens from the Brazilian turkey production must be conducted due to the hazardous risk that poxvirus infections may cause to the Brazilian poultry production scenario, given that Brazil's turkey production attracts attention due to its economic importance worldwide. Our findings point to the need to identify the prevalence of APV in Brazilian turkey production, to perform risk assessment studies and continued surveillance of APV infections in both wild and commercial avian species.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000500357
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000500357
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2016000500001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.36 n.5 2016
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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