Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis

Detalhes bibliográficos
Autor(a) principal: Resende,Cláudia F.
Data de Publicação: 2020
Outros Autores: Galinari,Grazielle C. F., Victor,Raphael M., Kassar,Telissa C., Arcebispo,Thiago L.M., Delarmelina,Emília, Leite,Romulo C., Reis,Jenner K.P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2020001200977
Resumo: ABSTRACT: Enzootic bovine leukosis (EBL) is an infectious disease caused by bovine leukemia virus (BLV) that affects cattle worldwide. Agar gel immunodiffusion (AGID) was the reference test for EBL diagnosis for many years, but enzyme-linked immunosorbent assay (ELISA) showed higher sensitivity, was faster to perform, and resulted in an objective reading. However, the importation of ELISA kits is lengthy and expensive, and currently, no AGID kits are available in Brazil. The aim of this work was to standardize an indirect ELISA (iELISA) for EBL diagnosis using BLV antigens produced in Tadarida brasiliensis lung (Tb1Lu) cells, which are Bovine viral diarrhea virus (BVDV) free, unlike fetal lamb kidney (FLK) cells, currently used for this purpose. Following standardization, iELISA results were compared with those obtained by AGID and the commercial Chekit Leucose-Serum ELISA. Compared to AGID, iELISA had 94,44% sensitivity, 75.68% specificity, 79.10% positive predictive value (PPV) and 93.30% negative predictive value (NPV), with 84% concordance and a Kappa index of 0.699. Compared to the Chekit Leucose-Serum ELISA, iELISA showed 92.60% sensitivity, 87.09% specificity, 90.27% PPV and 90,00% NPV, with 90.27% concordance and a Kappa index of 0.801. Taking into account the high agreement with the traditional tests and the absence of non-specific reactions with BVDV, the developed assay could be used as diagnostic method to control EBL in Brazil.
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spelling Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosisiELISAbovineenzyme-linked immunosorbent assayEnzootic bovine leukosisdiagnosiscontrolcattleABSTRACT: Enzootic bovine leukosis (EBL) is an infectious disease caused by bovine leukemia virus (BLV) that affects cattle worldwide. Agar gel immunodiffusion (AGID) was the reference test for EBL diagnosis for many years, but enzyme-linked immunosorbent assay (ELISA) showed higher sensitivity, was faster to perform, and resulted in an objective reading. However, the importation of ELISA kits is lengthy and expensive, and currently, no AGID kits are available in Brazil. The aim of this work was to standardize an indirect ELISA (iELISA) for EBL diagnosis using BLV antigens produced in Tadarida brasiliensis lung (Tb1Lu) cells, which are Bovine viral diarrhea virus (BVDV) free, unlike fetal lamb kidney (FLK) cells, currently used for this purpose. Following standardization, iELISA results were compared with those obtained by AGID and the commercial Chekit Leucose-Serum ELISA. Compared to AGID, iELISA had 94,44% sensitivity, 75.68% specificity, 79.10% positive predictive value (PPV) and 93.30% negative predictive value (NPV), with 84% concordance and a Kappa index of 0.699. Compared to the Chekit Leucose-Serum ELISA, iELISA showed 92.60% sensitivity, 87.09% specificity, 90.27% PPV and 90,00% NPV, with 90.27% concordance and a Kappa index of 0.801. Taking into account the high agreement with the traditional tests and the absence of non-specific reactions with BVDV, the developed assay could be used as diagnostic method to control EBL in Brazil.Colégio Brasileiro de Patologia Animal - CBPA2020-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2020001200977Pesquisa Veterinária Brasileira v.40 n.12 2020reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-6731info:eu-repo/semantics/openAccessResende,Cláudia F.Galinari,Grazielle C. F.Victor,Raphael M.Kassar,Telissa C.Arcebispo,Thiago L.M.Delarmelina,EmíliaLeite,Romulo C.Reis,Jenner K.P.eng2021-03-10T00:00:00Zoai:scielo:S0100-736X2020001200977Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2021-03-10T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis
title Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis
spellingShingle Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis
Resende,Cláudia F.
iELISA
bovine
enzyme-linked immunosorbent assay
Enzootic bovine leukosis
diagnosis
control
cattle
title_short Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis
title_full Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis
title_fullStr Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis
title_full_unstemmed Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis
title_sort Indirect ELISA (iELISA) standardization for the diagnosis of bovine enzootic leukosis
author Resende,Cláudia F.
author_facet Resende,Cláudia F.
Galinari,Grazielle C. F.
Victor,Raphael M.
Kassar,Telissa C.
Arcebispo,Thiago L.M.
Delarmelina,Emília
Leite,Romulo C.
Reis,Jenner K.P.
author_role author
author2 Galinari,Grazielle C. F.
Victor,Raphael M.
Kassar,Telissa C.
Arcebispo,Thiago L.M.
Delarmelina,Emília
Leite,Romulo C.
Reis,Jenner K.P.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Resende,Cláudia F.
Galinari,Grazielle C. F.
Victor,Raphael M.
Kassar,Telissa C.
Arcebispo,Thiago L.M.
Delarmelina,Emília
Leite,Romulo C.
Reis,Jenner K.P.
dc.subject.por.fl_str_mv iELISA
bovine
enzyme-linked immunosorbent assay
Enzootic bovine leukosis
diagnosis
control
cattle
topic iELISA
bovine
enzyme-linked immunosorbent assay
Enzootic bovine leukosis
diagnosis
control
cattle
description ABSTRACT: Enzootic bovine leukosis (EBL) is an infectious disease caused by bovine leukemia virus (BLV) that affects cattle worldwide. Agar gel immunodiffusion (AGID) was the reference test for EBL diagnosis for many years, but enzyme-linked immunosorbent assay (ELISA) showed higher sensitivity, was faster to perform, and resulted in an objective reading. However, the importation of ELISA kits is lengthy and expensive, and currently, no AGID kits are available in Brazil. The aim of this work was to standardize an indirect ELISA (iELISA) for EBL diagnosis using BLV antigens produced in Tadarida brasiliensis lung (Tb1Lu) cells, which are Bovine viral diarrhea virus (BVDV) free, unlike fetal lamb kidney (FLK) cells, currently used for this purpose. Following standardization, iELISA results were compared with those obtained by AGID and the commercial Chekit Leucose-Serum ELISA. Compared to AGID, iELISA had 94,44% sensitivity, 75.68% specificity, 79.10% positive predictive value (PPV) and 93.30% negative predictive value (NPV), with 84% concordance and a Kappa index of 0.699. Compared to the Chekit Leucose-Serum ELISA, iELISA showed 92.60% sensitivity, 87.09% specificity, 90.27% PPV and 90,00% NPV, with 90.27% concordance and a Kappa index of 0.801. Taking into account the high agreement with the traditional tests and the absence of non-specific reactions with BVDV, the developed assay could be used as diagnostic method to control EBL in Brazil.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2020001200977
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2020001200977
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-5150-pvb-6731
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.40 n.12 2020
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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