Effects of atracurium besylate on corneal endothelium of chickens: in vitro study
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000100070 |
Resumo: | ABSTRACT: The aim of this study was to investigate the acute effects of atracurium besylate on cellular damage in corneal endothelium of chickens. Twenty healthy chicken eyes were assigned to the following groups: Group 1 (G1), experimental group (n=10); and Group 2 (G2), control (n=10). Excised corneoscleral buttons were immediately placed on glass microscopy slides with endothelial region faced up. Corneal endothelium of eyes in G1 were covered with AB (0.2mL, 10mg/mL) for 3 min and then rinsed with balanced salt solution (BSS), while the corneal endothelium of eyes in G2 were covered with BBS for 3 min. Corneas from both groups were stained with alizarin red/trypan blue and visualized by light microscopy. Ten random photographs were taken from each cornea. The area of cellular damage was measured by software in all samples and cell loss of each group was averaged and compared. Endothelial area of denudation and Descemet’s membrane exposure were higher in G1 than G2. In conclusion, atracurium besylate induced an acute damage on corneal endothelium of chickens. |
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Effects of atracurium besylate on corneal endothelium of chickens: in vitro studyAtracurium besylatecorneal endotheliumchickensin vitroavianmydriasisdamagealizarin redABSTRACT: The aim of this study was to investigate the acute effects of atracurium besylate on cellular damage in corneal endothelium of chickens. Twenty healthy chicken eyes were assigned to the following groups: Group 1 (G1), experimental group (n=10); and Group 2 (G2), control (n=10). Excised corneoscleral buttons were immediately placed on glass microscopy slides with endothelial region faced up. Corneal endothelium of eyes in G1 were covered with AB (0.2mL, 10mg/mL) for 3 min and then rinsed with balanced salt solution (BSS), while the corneal endothelium of eyes in G2 were covered with BBS for 3 min. Corneas from both groups were stained with alizarin red/trypan blue and visualized by light microscopy. Ten random photographs were taken from each cornea. The area of cellular damage was measured by software in all samples and cell loss of each group was averaged and compared. Endothelial area of denudation and Descemet’s membrane exposure were higher in G1 than G2. In conclusion, atracurium besylate induced an acute damage on corneal endothelium of chickens.Colégio Brasileiro de Patologia Animal - CBPA2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000100070Pesquisa Veterinária Brasileira v.39 n.1 2019reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-5595info:eu-repo/semantics/openAccessGuimarães,Celeste B.Albuquerque,LucianeTorikachvili,MarcelaVargas,Eduarda V.Dall’Agnol,Cecilia C.Silva,Tanise C.Pigatto,João A.T.eng2019-03-19T00:00:00Zoai:scielo:S0100-736X2019000100070Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2019-03-19T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Effects of atracurium besylate on corneal endothelium of chickens: in vitro study |
title |
Effects of atracurium besylate on corneal endothelium of chickens: in vitro study |
spellingShingle |
Effects of atracurium besylate on corneal endothelium of chickens: in vitro study Guimarães,Celeste B. Atracurium besylate corneal endothelium chickens in vitro avian mydriasis damage alizarin red |
title_short |
Effects of atracurium besylate on corneal endothelium of chickens: in vitro study |
title_full |
Effects of atracurium besylate on corneal endothelium of chickens: in vitro study |
title_fullStr |
Effects of atracurium besylate on corneal endothelium of chickens: in vitro study |
title_full_unstemmed |
Effects of atracurium besylate on corneal endothelium of chickens: in vitro study |
title_sort |
Effects of atracurium besylate on corneal endothelium of chickens: in vitro study |
author |
Guimarães,Celeste B. |
author_facet |
Guimarães,Celeste B. Albuquerque,Luciane Torikachvili,Marcela Vargas,Eduarda V. Dall’Agnol,Cecilia C. Silva,Tanise C. Pigatto,João A.T. |
author_role |
author |
author2 |
Albuquerque,Luciane Torikachvili,Marcela Vargas,Eduarda V. Dall’Agnol,Cecilia C. Silva,Tanise C. Pigatto,João A.T. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Guimarães,Celeste B. Albuquerque,Luciane Torikachvili,Marcela Vargas,Eduarda V. Dall’Agnol,Cecilia C. Silva,Tanise C. Pigatto,João A.T. |
dc.subject.por.fl_str_mv |
Atracurium besylate corneal endothelium chickens in vitro avian mydriasis damage alizarin red |
topic |
Atracurium besylate corneal endothelium chickens in vitro avian mydriasis damage alizarin red |
description |
ABSTRACT: The aim of this study was to investigate the acute effects of atracurium besylate on cellular damage in corneal endothelium of chickens. Twenty healthy chicken eyes were assigned to the following groups: Group 1 (G1), experimental group (n=10); and Group 2 (G2), control (n=10). Excised corneoscleral buttons were immediately placed on glass microscopy slides with endothelial region faced up. Corneal endothelium of eyes in G1 were covered with AB (0.2mL, 10mg/mL) for 3 min and then rinsed with balanced salt solution (BSS), while the corneal endothelium of eyes in G2 were covered with BBS for 3 min. Corneas from both groups were stained with alizarin red/trypan blue and visualized by light microscopy. Ten random photographs were taken from each cornea. The area of cellular damage was measured by software in all samples and cell loss of each group was averaged and compared. Endothelial area of denudation and Descemet’s membrane exposure were higher in G1 than G2. In conclusion, atracurium besylate induced an acute damage on corneal endothelium of chickens. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000100070 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2019000100070 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-5150-pvb-5595 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.39 n.1 2019 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122239402311680 |