Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000200008 |
Resumo: | The standard method for detection of bovine tuberculosis (TB) is the single intradermal tuberculin test (SITT). Nevertheless, current studies suggest that a single test is not enough to detect all cattle infected by TB, particularly when animals present different stages of infection. A dairy herd comprised of 270 cows was studied and 15 were reactive to SITT plus nine inconclusive animals. Blood samples (for IFN and ELISA) were collected from these 24 cows. At 30 days after injection of PPD, all the cows that were reactive to any of the employed tests were slaughtered, and tissues were processed by Bacteriology, Histopathology (HP) and PCR. According to HP 33.4% of the animals were positive, 45.8% inconclusive and 20.8% were negative. The inconclusive samples came from IFN positive animals, signalizing recent infection. Regarding the animals that were negative to HP, all of them were identified by IFN while ELISA was negative. Immune responses are different in recent and advanced infections, what supports the identification between chronically or recently infected animals. This multidisciplinary approach is mandatory for the interpretation of the various tools that are frequently employed for the diagnosis of TB and mainly to identify all infected animals. |
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Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herdBovine tuberculosisdiagnosismultidisciplinary approachhistopathologyThe standard method for detection of bovine tuberculosis (TB) is the single intradermal tuberculin test (SITT). Nevertheless, current studies suggest that a single test is not enough to detect all cattle infected by TB, particularly when animals present different stages of infection. A dairy herd comprised of 270 cows was studied and 15 were reactive to SITT plus nine inconclusive animals. Blood samples (for IFN and ELISA) were collected from these 24 cows. At 30 days after injection of PPD, all the cows that were reactive to any of the employed tests were slaughtered, and tissues were processed by Bacteriology, Histopathology (HP) and PCR. According to HP 33.4% of the animals were positive, 45.8% inconclusive and 20.8% were negative. The inconclusive samples came from IFN positive animals, signalizing recent infection. Regarding the animals that were negative to HP, all of them were identified by IFN while ELISA was negative. Immune responses are different in recent and advanced infections, what supports the identification between chronically or recently infected animals. This multidisciplinary approach is mandatory for the interpretation of the various tools that are frequently employed for the diagnosis of TB and mainly to identify all infected animals.Colégio Brasileiro de Patologia Animal - CBPA2012-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000200008Pesquisa Veterinária Brasileira v.32 n.2 2012reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2012000200008info:eu-repo/semantics/openAccessMedeiros,Luciana S.Marassi,Carla D.Figueiredo,Eduardo E.SLeite,JulianaFerreira,Ana Maria R.Lilenbaum,Waltereng2012-04-03T00:00:00Zoai:scielo:S0100-736X2012000200008Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2012-04-03T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd |
title |
Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd |
spellingShingle |
Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd Medeiros,Luciana S. Bovine tuberculosis diagnosis multidisciplinary approach histopathology |
title_short |
Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd |
title_full |
Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd |
title_fullStr |
Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd |
title_full_unstemmed |
Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd |
title_sort |
Assessing the histopathology to depict the different stages of bovine tuberculosis infection in a naturally infected herd |
author |
Medeiros,Luciana S. |
author_facet |
Medeiros,Luciana S. Marassi,Carla D. Figueiredo,Eduardo E.S Leite,Juliana Ferreira,Ana Maria R. Lilenbaum,Walter |
author_role |
author |
author2 |
Marassi,Carla D. Figueiredo,Eduardo E.S Leite,Juliana Ferreira,Ana Maria R. Lilenbaum,Walter |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Medeiros,Luciana S. Marassi,Carla D. Figueiredo,Eduardo E.S Leite,Juliana Ferreira,Ana Maria R. Lilenbaum,Walter |
dc.subject.por.fl_str_mv |
Bovine tuberculosis diagnosis multidisciplinary approach histopathology |
topic |
Bovine tuberculosis diagnosis multidisciplinary approach histopathology |
description |
The standard method for detection of bovine tuberculosis (TB) is the single intradermal tuberculin test (SITT). Nevertheless, current studies suggest that a single test is not enough to detect all cattle infected by TB, particularly when animals present different stages of infection. A dairy herd comprised of 270 cows was studied and 15 were reactive to SITT plus nine inconclusive animals. Blood samples (for IFN and ELISA) were collected from these 24 cows. At 30 days after injection of PPD, all the cows that were reactive to any of the employed tests were slaughtered, and tissues were processed by Bacteriology, Histopathology (HP) and PCR. According to HP 33.4% of the animals were positive, 45.8% inconclusive and 20.8% were negative. The inconclusive samples came from IFN positive animals, signalizing recent infection. Regarding the animals that were negative to HP, all of them were identified by IFN while ELISA was negative. Immune responses are different in recent and advanced infections, what supports the identification between chronically or recently infected animals. This multidisciplinary approach is mandatory for the interpretation of the various tools that are frequently employed for the diagnosis of TB and mainly to identify all infected animals. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000200008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000200008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2012000200008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.32 n.2 2012 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122231806427136 |