Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies

Detalhes bibliográficos
Autor(a) principal: Mazur,Carlos
Data de Publicação: 2010
Outros Autores: Reis,Jenner K.P, Leite,Romulo C, Danelli,Maria das Graças M, Hagiwara,Mitika K, Góes,Ana Carolina M.A. de, Medeiros,Marcos A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000011
Resumo: Feline Immunodeficiency Virus is a worldwide infection and is considered a significant pathogen. The diagnosis of FIV infections is mainly based on commercially available rapid tests that are highly expensive in Brazil, hence it is rarely performed in the country. Furthermore, lentiviruses grow slowly and poorly in tissue cultures, making the production of viral antigen by classic means and thus the establishment of FIV immunodiagnosis impracticable. In order to deal with this, recombinant DNA techniques were adopted to produce the protein p24, a viral capsid antigen. The protein's reactivity evaluation analyzed by Western blot indicated that this recombinant antigen can be a useful tool for the immunodiagnostic of FIV infections.
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spelling Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodiesFIVfeline immunodeficiency viruscatsFeline Immunodeficiency Virus is a worldwide infection and is considered a significant pathogen. The diagnosis of FIV infections is mainly based on commercially available rapid tests that are highly expensive in Brazil, hence it is rarely performed in the country. Furthermore, lentiviruses grow slowly and poorly in tissue cultures, making the production of viral antigen by classic means and thus the establishment of FIV immunodiagnosis impracticable. In order to deal with this, recombinant DNA techniques were adopted to produce the protein p24, a viral capsid antigen. The protein's reactivity evaluation analyzed by Western blot indicated that this recombinant antigen can be a useful tool for the immunodiagnostic of FIV infections.Colégio Brasileiro de Patologia Animal - CBPA2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000011Pesquisa Veterinária Brasileira v.30 n.10 2010reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2010001000011info:eu-repo/semantics/openAccessMazur,CarlosReis,Jenner K.PLeite,Romulo CDanelli,Maria das Graças MHagiwara,Mitika KGóes,Ana Carolina M.A. deMedeiros,Marcos Aeng2010-12-06T00:00:00Zoai:scielo:S0100-736X2010001000011Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2010-12-06T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies
title Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies
spellingShingle Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies
Mazur,Carlos
FIV
feline immunodeficiency virus
cats
title_short Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies
title_full Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies
title_fullStr Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies
title_full_unstemmed Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies
title_sort Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies
author Mazur,Carlos
author_facet Mazur,Carlos
Reis,Jenner K.P
Leite,Romulo C
Danelli,Maria das Graças M
Hagiwara,Mitika K
Góes,Ana Carolina M.A. de
Medeiros,Marcos A
author_role author
author2 Reis,Jenner K.P
Leite,Romulo C
Danelli,Maria das Graças M
Hagiwara,Mitika K
Góes,Ana Carolina M.A. de
Medeiros,Marcos A
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mazur,Carlos
Reis,Jenner K.P
Leite,Romulo C
Danelli,Maria das Graças M
Hagiwara,Mitika K
Góes,Ana Carolina M.A. de
Medeiros,Marcos A
dc.subject.por.fl_str_mv FIV
feline immunodeficiency virus
cats
topic FIV
feline immunodeficiency virus
cats
description Feline Immunodeficiency Virus is a worldwide infection and is considered a significant pathogen. The diagnosis of FIV infections is mainly based on commercially available rapid tests that are highly expensive in Brazil, hence it is rarely performed in the country. Furthermore, lentiviruses grow slowly and poorly in tissue cultures, making the production of viral antigen by classic means and thus the establishment of FIV immunodiagnosis impracticable. In order to deal with this, recombinant DNA techniques were adopted to produce the protein p24, a viral capsid antigen. The protein's reactivity evaluation analyzed by Western blot indicated that this recombinant antigen can be a useful tool for the immunodiagnostic of FIV infections.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2010001000011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.30 n.10 2010
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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