Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000300387 |
Resumo: | ABSTRACT: The present study performed a genetic identification of pestiviruses contaminating batches of fetal bovine serum (FBS) produced in Brazil from 2006 to 2014. Seventy-three FBS lots were screened by a RT-PCR targeting the 5’untranslated region (UTR) of the pestivirus genome. Thirty-nine lots (53.4%) were positive for pestivirus RNA and one contained infectious virus. Nucleotide sequencing and phylogenetic analysis of the 5’UTR revealed 34 lots (46.6%) containing RNA of bovine viral diarrhea virus type 1 (BVDV-1), being 23 BVDV-1a (5’ UTR identity 90.8-98.7%), eight BVDV-1b (93.9-96.7%) and three BVDV-1d (96.2- 97.6%). Six lots (8.2%) contained BVDV-2 (90.3-100% UTR identity) being two BVDV-2a; three BVDV-2b and one undetermined. Four FBS batches (5.5%) were found contaminated with HoBi-like virus (98.3 to 100%). Five batches (6.8%) contained more than one pestivirus. The high frequency of contamination of FBS with pestivirus RNA reinforce the need for systematic and updated guidelines for monitoring this product to reduce the risk of contamination of biologicals and introduction of contaminating agents into free areas. |
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Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014Genetic identificationpestivirusesfetal bovine serumBVDVpestiviruscontaminationcattleviroses.ABSTRACT: The present study performed a genetic identification of pestiviruses contaminating batches of fetal bovine serum (FBS) produced in Brazil from 2006 to 2014. Seventy-three FBS lots were screened by a RT-PCR targeting the 5’untranslated region (UTR) of the pestivirus genome. Thirty-nine lots (53.4%) were positive for pestivirus RNA and one contained infectious virus. Nucleotide sequencing and phylogenetic analysis of the 5’UTR revealed 34 lots (46.6%) containing RNA of bovine viral diarrhea virus type 1 (BVDV-1), being 23 BVDV-1a (5’ UTR identity 90.8-98.7%), eight BVDV-1b (93.9-96.7%) and three BVDV-1d (96.2- 97.6%). Six lots (8.2%) contained BVDV-2 (90.3-100% UTR identity) being two BVDV-2a; three BVDV-2b and one undetermined. Four FBS batches (5.5%) were found contaminated with HoBi-like virus (98.3 to 100%). Five batches (6.8%) contained more than one pestivirus. The high frequency of contamination of FBS with pestivirus RNA reinforce the need for systematic and updated guidelines for monitoring this product to reduce the risk of contamination of biologicals and introduction of contaminating agents into free areas.Colégio Brasileiro de Patologia Animal - CBPA2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000300387Pesquisa Veterinária Brasileira v.38 n.3 2018reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-5283info:eu-repo/semantics/openAccessMonteiro,Francielle L.Cargnelutti,Juliana F.Braunig,PatríciaFolgueras-Flatschart,Aurea V.Santos,Nathália C.Pituco,Edviges M.Weiblen,RudiFlores,Eduardo F.eng2018-06-06T00:00:00Zoai:scielo:S0100-736X2018000300387Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2018-06-06T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014 |
title |
Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014 |
spellingShingle |
Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014 Monteiro,Francielle L. Genetic identification pestiviruses fetal bovine serum BVDV pestivirus contamination cattle viroses. |
title_short |
Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014 |
title_full |
Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014 |
title_fullStr |
Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014 |
title_full_unstemmed |
Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014 |
title_sort |
Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014 |
author |
Monteiro,Francielle L. |
author_facet |
Monteiro,Francielle L. Cargnelutti,Juliana F. Braunig,Patrícia Folgueras-Flatschart,Aurea V. Santos,Nathália C. Pituco,Edviges M. Weiblen,Rudi Flores,Eduardo F. |
author_role |
author |
author2 |
Cargnelutti,Juliana F. Braunig,Patrícia Folgueras-Flatschart,Aurea V. Santos,Nathália C. Pituco,Edviges M. Weiblen,Rudi Flores,Eduardo F. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Monteiro,Francielle L. Cargnelutti,Juliana F. Braunig,Patrícia Folgueras-Flatschart,Aurea V. Santos,Nathália C. Pituco,Edviges M. Weiblen,Rudi Flores,Eduardo F. |
dc.subject.por.fl_str_mv |
Genetic identification pestiviruses fetal bovine serum BVDV pestivirus contamination cattle viroses. |
topic |
Genetic identification pestiviruses fetal bovine serum BVDV pestivirus contamination cattle viroses. |
description |
ABSTRACT: The present study performed a genetic identification of pestiviruses contaminating batches of fetal bovine serum (FBS) produced in Brazil from 2006 to 2014. Seventy-three FBS lots were screened by a RT-PCR targeting the 5’untranslated region (UTR) of the pestivirus genome. Thirty-nine lots (53.4%) were positive for pestivirus RNA and one contained infectious virus. Nucleotide sequencing and phylogenetic analysis of the 5’UTR revealed 34 lots (46.6%) containing RNA of bovine viral diarrhea virus type 1 (BVDV-1), being 23 BVDV-1a (5’ UTR identity 90.8-98.7%), eight BVDV-1b (93.9-96.7%) and three BVDV-1d (96.2- 97.6%). Six lots (8.2%) contained BVDV-2 (90.3-100% UTR identity) being two BVDV-2a; three BVDV-2b and one undetermined. Four FBS batches (5.5%) were found contaminated with HoBi-like virus (98.3 to 100%). Five batches (6.8%) contained more than one pestivirus. The high frequency of contamination of FBS with pestivirus RNA reinforce the need for systematic and updated guidelines for monitoring this product to reduce the risk of contamination of biologicals and introduction of contaminating agents into free areas. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000300387 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000300387 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-5150-pvb-5283 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.38 n.3 2018 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122238007705600 |