Serological investigation and PCR in detection of pathogenic leptospires in snakes
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2011000900013 |
Resumo: | Detection of Leptospira by PCR had not yet been described in snakes. This study investigated, by microscopic agglutination test (MAT) and PCR, the presence of antibodies to Leptospira spp. and Leptospira spp., respectively, in venomous and non-venomous wildlife and captivity snakes. All snakes were divided into three groups to be compared: Group 1 (wildlife snakes - WS); Group 2 (snakes in intensive captivity - IC), and Group 3 (collective semi-extensive captivity -CC). Of the 147 snakes studied, 52 (35.4%) were positive for leptospirosis by MAT, 8 (15.4%) belonging to Group 1 (WS), 34 (65.4%) to Group 2 (IC) and 10 (19.2%) to Group 3 (CC). Jararaca (Bothrops jararaca) presented the highest average titer (66.7%, N=22/33) among the three group studied, and Hardjo prajtino was the most prevalent serovar (88.5%, N=46/52), with titers varying from 100 to 3200. Leptospira interrogans was revealed by PCR in kidney and liver of caiçaca (Bothrops moojeni) and jararaca-pintada (Bothrops pauloensis), showing 100% and 93% identity respectively. Future studies should be carried out for better understanding of the role of snakes as a reservoir of Leptospira in nature. |
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Serological investigation and PCR in detection of pathogenic leptospires in snakesPCRMATLeptospira spp.snakesBothrops jararacaBothrops moojeniHardjo prajtinoDetection of Leptospira by PCR had not yet been described in snakes. This study investigated, by microscopic agglutination test (MAT) and PCR, the presence of antibodies to Leptospira spp. and Leptospira spp., respectively, in venomous and non-venomous wildlife and captivity snakes. All snakes were divided into three groups to be compared: Group 1 (wildlife snakes - WS); Group 2 (snakes in intensive captivity - IC), and Group 3 (collective semi-extensive captivity -CC). Of the 147 snakes studied, 52 (35.4%) were positive for leptospirosis by MAT, 8 (15.4%) belonging to Group 1 (WS), 34 (65.4%) to Group 2 (IC) and 10 (19.2%) to Group 3 (CC). Jararaca (Bothrops jararaca) presented the highest average titer (66.7%, N=22/33) among the three group studied, and Hardjo prajtino was the most prevalent serovar (88.5%, N=46/52), with titers varying from 100 to 3200. Leptospira interrogans was revealed by PCR in kidney and liver of caiçaca (Bothrops moojeni) and jararaca-pintada (Bothrops pauloensis), showing 100% and 93% identity respectively. Future studies should be carried out for better understanding of the role of snakes as a reservoir of Leptospira in nature.Colégio Brasileiro de Patologia Animal - CBPA2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2011000900013Pesquisa Veterinária Brasileira v.31 n.9 2011reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2011000900013info:eu-repo/semantics/openAccessBiscola,Natália P.Fornazari,FelipeSaad,EduardoRichini-Pereira,Virginia B.Campagner,Michelle V.Langoni,HelioBarraviera,BeneditoFerreira Junior,Rui S.eng2011-10-13T00:00:00Zoai:scielo:S0100-736X2011000900013Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2011-10-13T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Serological investigation and PCR in detection of pathogenic leptospires in snakes |
title |
Serological investigation and PCR in detection of pathogenic leptospires in snakes |
spellingShingle |
Serological investigation and PCR in detection of pathogenic leptospires in snakes Biscola,Natália P. PCR MAT Leptospira spp. snakes Bothrops jararaca Bothrops moojeni Hardjo prajtino |
title_short |
Serological investigation and PCR in detection of pathogenic leptospires in snakes |
title_full |
Serological investigation and PCR in detection of pathogenic leptospires in snakes |
title_fullStr |
Serological investigation and PCR in detection of pathogenic leptospires in snakes |
title_full_unstemmed |
Serological investigation and PCR in detection of pathogenic leptospires in snakes |
title_sort |
Serological investigation and PCR in detection of pathogenic leptospires in snakes |
author |
Biscola,Natália P. |
author_facet |
Biscola,Natália P. Fornazari,Felipe Saad,Eduardo Richini-Pereira,Virginia B. Campagner,Michelle V. Langoni,Helio Barraviera,Benedito Ferreira Junior,Rui S. |
author_role |
author |
author2 |
Fornazari,Felipe Saad,Eduardo Richini-Pereira,Virginia B. Campagner,Michelle V. Langoni,Helio Barraviera,Benedito Ferreira Junior,Rui S. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Biscola,Natália P. Fornazari,Felipe Saad,Eduardo Richini-Pereira,Virginia B. Campagner,Michelle V. Langoni,Helio Barraviera,Benedito Ferreira Junior,Rui S. |
dc.subject.por.fl_str_mv |
PCR MAT Leptospira spp. snakes Bothrops jararaca Bothrops moojeni Hardjo prajtino |
topic |
PCR MAT Leptospira spp. snakes Bothrops jararaca Bothrops moojeni Hardjo prajtino |
description |
Detection of Leptospira by PCR had not yet been described in snakes. This study investigated, by microscopic agglutination test (MAT) and PCR, the presence of antibodies to Leptospira spp. and Leptospira spp., respectively, in venomous and non-venomous wildlife and captivity snakes. All snakes were divided into three groups to be compared: Group 1 (wildlife snakes - WS); Group 2 (snakes in intensive captivity - IC), and Group 3 (collective semi-extensive captivity -CC). Of the 147 snakes studied, 52 (35.4%) were positive for leptospirosis by MAT, 8 (15.4%) belonging to Group 1 (WS), 34 (65.4%) to Group 2 (IC) and 10 (19.2%) to Group 3 (CC). Jararaca (Bothrops jararaca) presented the highest average titer (66.7%, N=22/33) among the three group studied, and Hardjo prajtino was the most prevalent serovar (88.5%, N=46/52), with titers varying from 100 to 3200. Leptospira interrogans was revealed by PCR in kidney and liver of caiçaca (Bothrops moojeni) and jararaca-pintada (Bothrops pauloensis), showing 100% and 93% identity respectively. Future studies should be carried out for better understanding of the role of snakes as a reservoir of Leptospira in nature. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2011000900013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2011000900013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2011000900013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.31 n.9 2011 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122231357636608 |