Detection of specific antibodies in cows after injection of PPD

Detalhes bibliográficos
Autor(a) principal: Marassi,Carla D.
Data de Publicação: 2014
Outros Autores: Zarden,Carlos, Oelemann,Walter, Lilenbaum,Walter
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2014000300007
Resumo: The diagnosis of bovine tuberculosis aims to identify the immune response against mycobacterial antigens. Although Single Intradermal Comparative Cervical Tuberculin test (SICCT) is broadly used for first identification of the disease, the performance of ELISAs has been investigated for diagnosis improvement. The present study expected to find out the influence of intradermal skin tests on the results of ELISAs using the recombinant proteins MPB70 and MPB83 as antigens on cows from a naturally infected herd. Results were analyzed by the F-test, Mann-Whitney and Friedman tests Although comparable to both proteins, results showed that positive animals presented a tendency of augment reactivity to MPB70, representing a tendency for a booster effect, but not to MPB83.
id EMBRAPA-2_ede74a9d3f4727726ca1fda175d8888a
oai_identifier_str oai:scielo:S0100-736X2014000300007
network_acronym_str EMBRAPA-2
network_name_str Pesquisa Veterinária Brasileira (Online)
repository_id_str
spelling Detection of specific antibodies in cows after injection of PPDELISAtuberculosiscattleMPB70MPB83interferenceThe diagnosis of bovine tuberculosis aims to identify the immune response against mycobacterial antigens. Although Single Intradermal Comparative Cervical Tuberculin test (SICCT) is broadly used for first identification of the disease, the performance of ELISAs has been investigated for diagnosis improvement. The present study expected to find out the influence of intradermal skin tests on the results of ELISAs using the recombinant proteins MPB70 and MPB83 as antigens on cows from a naturally infected herd. Results were analyzed by the F-test, Mann-Whitney and Friedman tests Although comparable to both proteins, results showed that positive animals presented a tendency of augment reactivity to MPB70, representing a tendency for a booster effect, but not to MPB83.Colégio Brasileiro de Patologia Animal - CBPA2014-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2014000300007Pesquisa Veterinária Brasileira v.34 n.3 2014reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2014000300007info:eu-repo/semantics/openAccessMarassi,Carla D.Zarden,CarlosOelemann,WalterLilenbaum,Waltereng2014-05-16T00:00:00Zoai:scielo:S0100-736X2014000300007Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2014-05-16T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Detection of specific antibodies in cows after injection of PPD
title Detection of specific antibodies in cows after injection of PPD
spellingShingle Detection of specific antibodies in cows after injection of PPD
Marassi,Carla D.
ELISA
tuberculosis
cattle
MPB70
MPB83
interference
title_short Detection of specific antibodies in cows after injection of PPD
title_full Detection of specific antibodies in cows after injection of PPD
title_fullStr Detection of specific antibodies in cows after injection of PPD
title_full_unstemmed Detection of specific antibodies in cows after injection of PPD
title_sort Detection of specific antibodies in cows after injection of PPD
author Marassi,Carla D.
author_facet Marassi,Carla D.
Zarden,Carlos
Oelemann,Walter
Lilenbaum,Walter
author_role author
author2 Zarden,Carlos
Oelemann,Walter
Lilenbaum,Walter
author2_role author
author
author
dc.contributor.author.fl_str_mv Marassi,Carla D.
Zarden,Carlos
Oelemann,Walter
Lilenbaum,Walter
dc.subject.por.fl_str_mv ELISA
tuberculosis
cattle
MPB70
MPB83
interference
topic ELISA
tuberculosis
cattle
MPB70
MPB83
interference
description The diagnosis of bovine tuberculosis aims to identify the immune response against mycobacterial antigens. Although Single Intradermal Comparative Cervical Tuberculin test (SICCT) is broadly used for first identification of the disease, the performance of ELISAs has been investigated for diagnosis improvement. The present study expected to find out the influence of intradermal skin tests on the results of ELISAs using the recombinant proteins MPB70 and MPB83 as antigens on cows from a naturally infected herd. Results were analyzed by the F-test, Mann-Whitney and Friedman tests Although comparable to both proteins, results showed that positive animals presented a tendency of augment reactivity to MPB70, representing a tendency for a booster effect, but not to MPB83.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2014000300007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2014000300007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2014000300007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.34 n.3 2014
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
_version_ 1754122234172014592

Registros relacionados