cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian Amazon

Detalhes bibliográficos
Autor(a) principal: Azevedo, Vânia Cristina Rennó
Data de Publicação: 2008
Outros Autores: Kanashiro, Milton, Grattapaglia, Dario, Ciampi, Ana Yamaguishi
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/251
Resumo: The objective of this work was to evaluate the existence of matrilineal genetic structure in maçaranduba (Manilkara huberi). Evaluations were performed for 481 adult individuals of M. huberi, distributed in 200 hectares of a natural population in the Floresta Nacional do Tapajós, Belterra, PA, Brazil, and 88 plantules, based on nine chloroplast microsatellite loci and, of 96 individuals, selected randomly in the 200 ha area, it was carried out the sequencing of three intergenic regions from the cpDNA. No sequence polymorphism was detected. The analysis of the haplotypic variability showed a relatively low polymorphism, which resulted in 15 haplotypes, with total genetic diversity (hT) of 0.898. Significant genetic structure until 250 m was detected, which indicates that seeds are restrictedly dispersed and confirms the pattern of spatial organization of the genetic variability, showed by the nuclear DNA analysis. This indicates isolation by distance and the need of maintenance of primary forest large areas to allow the surviving of a greater number of subpopulations.
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spelling cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian AmazonVariabilidade no cpDNA em Manilkara huberi, espécie sob manejo sustentável na Amazônia brasileiraconservation; spatial genetic structure; maçaranduba; management; microsatellite; sequencingconservação; estruturação genética espacial; maçaranduba; manejo; microssatélite; seqüenciamentoThe objective of this work was to evaluate the existence of matrilineal genetic structure in maçaranduba (Manilkara huberi). Evaluations were performed for 481 adult individuals of M. huberi, distributed in 200 hectares of a natural population in the Floresta Nacional do Tapajós, Belterra, PA, Brazil, and 88 plantules, based on nine chloroplast microsatellite loci and, of 96 individuals, selected randomly in the 200 ha area, it was carried out the sequencing of three intergenic regions from the cpDNA. No sequence polymorphism was detected. The analysis of the haplotypic variability showed a relatively low polymorphism, which resulted in 15 haplotypes, with total genetic diversity (hT) of 0.898. Significant genetic structure until 250 m was detected, which indicates that seeds are restrictedly dispersed and confirms the pattern of spatial organization of the genetic variability, showed by the nuclear DNA analysis. This indicates isolation by distance and the need of maintenance of primary forest large areas to allow the surviving of a greater number of subpopulations.O objetivo deste trabalho foi avaliar a existência de estruturação genética matrilinear em maçaranduba (Manilkara huberi). Foram avaliados 481 indivíduos adultos de M. huberi, distribuídos em 200 hectares de uma população natural na Floresta Nacional do Tapajós, Belterra, PA, e 88 regenerantes, com base na análise de nove microssatélites de cloroplasto e, de 96 indivíduos adultos, selecionados aleatoriamente na área de 200 ha, foi realizado o seqüenciamento de três regiões não codificadoras de cpDNA. Não foi detectado polimorfismo de seqüência. A análise da variabilidade haplotípica mostrou polimorfismo relativamente limitado, que resultou em 15 haplótipos, com diversidade genética total (hT) de 0,898. Foi detectada a existência de estruturação genética significativa em distâncias de até 250 m, o que indica dispersão de sementes restrita e confirma o padrão de organização espacial da variabilidade genética mostrado pela análise de DNA nuclear, o que evidencia isolamento por distância e a necessidade de manutenção de grandes áreas de floresta primária para garantir a sobrevivência de maior número de subpopulações.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraAzevedo, Vânia Cristina RennóKanashiro, MiltonGrattapaglia, DarioCiampi, Ana Yamaguishi2008-10-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/251Pesquisa Agropecuaria Brasileira; v.43, n.7, jul. 2008; 859-867Pesquisa Agropecuária Brasileira; v.43, n.7, jul. 2008; 859-8671678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/251/5472info:eu-repo/semantics/openAccess2012-06-17T11:41:59Zoai:ojs.seer.sct.embrapa.br:article/251Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:41:59Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian Amazon
Variabilidade no cpDNA em Manilkara huberi, espécie sob manejo sustentável na Amazônia brasileira
title cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian Amazon
spellingShingle cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian Amazon
Azevedo, Vânia Cristina Rennó
conservation; spatial genetic structure; maçaranduba; management; microsatellite; sequencing
conservação; estruturação genética espacial; maçaranduba; manejo; microssatélite; seqüenciamento
title_short cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian Amazon
title_full cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian Amazon
title_fullStr cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian Amazon
title_full_unstemmed cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian Amazon
title_sort cpDNA variability in Manilkara huberi, a species under sustainable management in the Brazilian Amazon
author Azevedo, Vânia Cristina Rennó
author_facet Azevedo, Vânia Cristina Rennó
Kanashiro, Milton
Grattapaglia, Dario
Ciampi, Ana Yamaguishi
author_role author
author2 Kanashiro, Milton
Grattapaglia, Dario
Ciampi, Ana Yamaguishi
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Azevedo, Vânia Cristina Rennó
Kanashiro, Milton
Grattapaglia, Dario
Ciampi, Ana Yamaguishi
dc.subject.por.fl_str_mv conservation; spatial genetic structure; maçaranduba; management; microsatellite; sequencing
conservação; estruturação genética espacial; maçaranduba; manejo; microssatélite; seqüenciamento
topic conservation; spatial genetic structure; maçaranduba; management; microsatellite; sequencing
conservação; estruturação genética espacial; maçaranduba; manejo; microssatélite; seqüenciamento
description The objective of this work was to evaluate the existence of matrilineal genetic structure in maçaranduba (Manilkara huberi). Evaluations were performed for 481 adult individuals of M. huberi, distributed in 200 hectares of a natural population in the Floresta Nacional do Tapajós, Belterra, PA, Brazil, and 88 plantules, based on nine chloroplast microsatellite loci and, of 96 individuals, selected randomly in the 200 ha area, it was carried out the sequencing of three intergenic regions from the cpDNA. No sequence polymorphism was detected. The analysis of the haplotypic variability showed a relatively low polymorphism, which resulted in 15 haplotypes, with total genetic diversity (hT) of 0.898. Significant genetic structure until 250 m was detected, which indicates that seeds are restrictedly dispersed and confirms the pattern of spatial organization of the genetic variability, showed by the nuclear DNA analysis. This indicates isolation by distance and the need of maintenance of primary forest large areas to allow the surviving of a greater number of subpopulations.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-27
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/251
url https://seer.sct.embrapa.br/index.php/pab/article/view/251
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/251/5472
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.43, n.7, jul. 2008; 859-867
Pesquisa Agropecuária Brasileira; v.43, n.7, jul. 2008; 859-867
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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