Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching

Detalhes bibliográficos
Autor(a) principal: Gomes-Copeland, Kicia Karinne Pereira
Data de Publicação: 2012
Outros Autores: Lédo, Ana da Silva, Almeida, Fabrício Tavares Cunha de, Fernandes, Roberta Pereira Miranda, Santos, Izulmé Rita Imaculada
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/11491
Resumo: The objective of this work was to adapt the application of electrolytic conductivity and potassium leaching tests to assess the viability of cryopreserved embryos of 'Anão Verde do Brasil de Jiqui' (AVeJBr) coconut. The zygotic embryos were excised, sterilized and subjected to four cryoprotectant treatments combined with three incubation times (12, 16 and 20 hours), totaling 12 treatments. The pre‑treatment of mature zygotic embryos of AVeJBr coconut using a cryoprotectant with 1.75 mol L‑1 of sucrose + 15% glycerol for 12 and 16 hours promoted lower embryo humidity and increased viability in electrolytic conductivity and potassium leaching tests. Samples with ten embryos are sufficient for electrolytic conductivity analysis in cryopreserved or non‑cryopreserved AVeJBr coconut zygotic embryos. The 4 to 8 hour imbibition period of the embryos is promising for the electrolytic conductivity analysis of non‑cryopreserved mature zygotic embryos of AVeJBr coconut.
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spelling Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leachingAvaliação da viabilidade de embriões de coqueiro criopreservados por meio de condutividade elétrica e lixiviação de potássioCocos nucifera; biochemistry; cell membrane; cryoprotectant; genetic resourcesCocos nucifera; bioquímica; membrana celular; crioprotetor; recursos genéticosThe objective of this work was to adapt the application of electrolytic conductivity and potassium leaching tests to assess the viability of cryopreserved embryos of 'Anão Verde do Brasil de Jiqui' (AVeJBr) coconut. The zygotic embryos were excised, sterilized and subjected to four cryoprotectant treatments combined with three incubation times (12, 16 and 20 hours), totaling 12 treatments. The pre‑treatment of mature zygotic embryos of AVeJBr coconut using a cryoprotectant with 1.75 mol L‑1 of sucrose + 15% glycerol for 12 and 16 hours promoted lower embryo humidity and increased viability in electrolytic conductivity and potassium leaching tests. Samples with ten embryos are sufficient for electrolytic conductivity analysis in cryopreserved or non‑cryopreserved AVeJBr coconut zygotic embryos. The 4 to 8 hour imbibition period of the embryos is promising for the electrolytic conductivity analysis of non‑cryopreserved mature zygotic embryos of AVeJBr coconut.O objetivo deste trabalho foi adaptar a aplicação de testes de condutividade elétrica e lixiviação de potássio para avaliar a viabilidade de embriões de coqueiro 'Anão Verde do Brasil de Jiqui' (AVeJBr) criopreservados. Os embriões zigóticos foram excisados, esterilizados e submetidos a quatro tratamentos crioprotetores combinados a três tempos de incubação (12, 16 e 20 horas), o que totalizou 12 tratamentos. O pré‑tratamento de embriões zigóticos maduros de coco AVeJBr com crioprotetor contendo 1,75 mol L‑1 de sacarose + 15% de glicerol por 12 e 16 horas promoveu menor umidade dos embriões e maior viabilidade em testes de condutividade elétrica e lixiviação de potássio. Amostras com dez embriões são suficientes para análise de condutividade elétrica em embriões zigóticos de coqueiro AVeJBr criopreservados ou não. O período de embebição de 4 a 8 horas é promissor para a análise da condutividade elétrica em embriões zigóticosmaduros de coco AVeJBr não criopreservados.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPq, Embrapa, Cogent/Bioversity InternationalGomes-Copeland, Kicia Karinne PereiraLédo, Ana da SilvaAlmeida, Fabrício Tavares Cunha deFernandes, Roberta Pereira MirandaSantos, Izulmé Rita Imaculada2012-03-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/11491Pesquisa Agropecuaria Brasileira; v.47, n.1, jan. 2012; 8-13Pesquisa Agropecuária Brasileira; v.47, n.1, jan. 2012; 8-131678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/11491/6744https://seer.sct.embrapa.br/index.php/pab/article/view/11491/7414https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11491/7064https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11491/7068https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11491/7069https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11491/7140info:eu-repo/semantics/openAccess2012-06-17T12:18:04Zoai:ojs.seer.sct.embrapa.br:article/11491Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T12:18:04Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching
Avaliação da viabilidade de embriões de coqueiro criopreservados por meio de condutividade elétrica e lixiviação de potássio
title Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching
spellingShingle Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching
Gomes-Copeland, Kicia Karinne Pereira
Cocos nucifera; biochemistry; cell membrane; cryoprotectant; genetic resources
Cocos nucifera; bioquímica; membrana celular; crioprotetor; recursos genéticos
title_short Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching
title_full Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching
title_fullStr Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching
title_full_unstemmed Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching
title_sort Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching
author Gomes-Copeland, Kicia Karinne Pereira
author_facet Gomes-Copeland, Kicia Karinne Pereira
Lédo, Ana da Silva
Almeida, Fabrício Tavares Cunha de
Fernandes, Roberta Pereira Miranda
Santos, Izulmé Rita Imaculada
author_role author
author2 Lédo, Ana da Silva
Almeida, Fabrício Tavares Cunha de
Fernandes, Roberta Pereira Miranda
Santos, Izulmé Rita Imaculada
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
CNPq, Embrapa, Cogent/Bioversity International
dc.contributor.author.fl_str_mv Gomes-Copeland, Kicia Karinne Pereira
Lédo, Ana da Silva
Almeida, Fabrício Tavares Cunha de
Fernandes, Roberta Pereira Miranda
Santos, Izulmé Rita Imaculada
dc.subject.por.fl_str_mv Cocos nucifera; biochemistry; cell membrane; cryoprotectant; genetic resources
Cocos nucifera; bioquímica; membrana celular; crioprotetor; recursos genéticos
topic Cocos nucifera; biochemistry; cell membrane; cryoprotectant; genetic resources
Cocos nucifera; bioquímica; membrana celular; crioprotetor; recursos genéticos
description The objective of this work was to adapt the application of electrolytic conductivity and potassium leaching tests to assess the viability of cryopreserved embryos of 'Anão Verde do Brasil de Jiqui' (AVeJBr) coconut. The zygotic embryos were excised, sterilized and subjected to four cryoprotectant treatments combined with three incubation times (12, 16 and 20 hours), totaling 12 treatments. The pre‑treatment of mature zygotic embryos of AVeJBr coconut using a cryoprotectant with 1.75 mol L‑1 of sucrose + 15% glycerol for 12 and 16 hours promoted lower embryo humidity and increased viability in electrolytic conductivity and potassium leaching tests. Samples with ten embryos are sufficient for electrolytic conductivity analysis in cryopreserved or non‑cryopreserved AVeJBr coconut zygotic embryos. The 4 to 8 hour imbibition period of the embryos is promising for the electrolytic conductivity analysis of non‑cryopreserved mature zygotic embryos of AVeJBr coconut.
publishDate 2012
dc.date.none.fl_str_mv 2012-03-16
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/11491
url https://seer.sct.embrapa.br/index.php/pab/article/view/11491
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/11491/6744
https://seer.sct.embrapa.br/index.php/pab/article/view/11491/7414
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11491/7064
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11491/7068
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11491/7069
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11491/7140
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.47, n.1, jan. 2012; 8-13
Pesquisa Agropecuária Brasileira; v.47, n.1, jan. 2012; 8-13
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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