Storage protein profile and amino acid content in wild rice Oryza glumaepatula

Detalhes bibliográficos
Autor(a) principal: Santos, Karina Freire D’eça Nogueira
Data de Publicação: 2013
Outros Autores: Silveira, Ricardo Diógenes Dias, Martin-Didonet, Claudia Cristina Garcia, Brondani, Claudio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/12784
Resumo: The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan) in comparison to the two analized rice cultivars. The albumin and glutelin profiles in SDS-Page were distinct between rice cultivars and O. glumaepatula. This wild species has the potential to increase the nutritional quality of rice storage protein through interspecific crosses.
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spelling Storage protein profile and amino acid content in wild rice Oryza glumaepatulaPerfil da proteína de reserva e conteúdo de aminoácidos no arroz silvestre Oryza glumaepatulaOryza sativa; genetic resources; nutritional quality; plant breeding; SDS‑Page.Oryza sativa; recursos genéticos; qualidade nutricional; melhoramento de plantas; SDS‑Page.The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan) in comparison to the two analized rice cultivars. The albumin and glutelin profiles in SDS-Page were distinct between rice cultivars and O. glumaepatula. This wild species has the potential to increase the nutritional quality of rice storage protein through interspecific crosses.O objetivo deste trabalho foi determinar os perfis de proteína total e o conteúdo das quatro principais frações proteicas (albumina, globulina, prolamina e glutelina) e de aminoácidos no endosperma da espécie de arroz silvestre Oryza glumaepatula. O experimento foi realizado com 29 acessos dessa espécie, coletados em 13 locais no Brasil, e duas cultivares comerciais. Amostras de proteínas foram preparadas com grãos secos, polidos e moídos, para obtenção de uma farinha seca e homogênea, usada no preparo dos estratos. Acessos de O. glumaepatula foram identificados com os maiores níveis de proteína total, frações proteicas de albumina e glutelina, e aminoácidos (com exceção do triptofano), em comparação às duas cultivares de arroz avaliadas. Os perfis de albumina e glutelina em SDS-PAGE foram distintos entre as cultivares de arroz e O. glumaepatula. Essa espécie silvestre tem o potencial de aumentar a qualidade nutricional da proteína de reserva do arroz por meio de cruzamentos interespecíficos.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCAPES and CNPqSantos, Karina Freire D’eça NogueiraSilveira, Ricardo Diógenes DiasMartin-Didonet, Claudia Cristina GarciaBrondani, Claudio2013-03-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/12784Pesquisa Agropecuaria Brasileira; v.48, n.1, jan. 2013; 66-72Pesquisa Agropecuária Brasileira; v.48, n.1, jan. 2013; 66-721678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/12784/8970https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12784/8486https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12784/8487https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12784/8488https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12784/8489info:eu-repo/semantics/openAccess2013-03-19T17:46:31Zoai:ojs.seer.sct.embrapa.br:article/12784Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2013-03-19T17:46:31Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Storage protein profile and amino acid content in wild rice Oryza glumaepatula
Perfil da proteína de reserva e conteúdo de aminoácidos no arroz silvestre Oryza glumaepatula
title Storage protein profile and amino acid content in wild rice Oryza glumaepatula
spellingShingle Storage protein profile and amino acid content in wild rice Oryza glumaepatula
Santos, Karina Freire D’eça Nogueira
Oryza sativa; genetic resources; nutritional quality; plant breeding; SDS‑Page.
Oryza sativa; recursos genéticos; qualidade nutricional; melhoramento de plantas; SDS‑Page.
title_short Storage protein profile and amino acid content in wild rice Oryza glumaepatula
title_full Storage protein profile and amino acid content in wild rice Oryza glumaepatula
title_fullStr Storage protein profile and amino acid content in wild rice Oryza glumaepatula
title_full_unstemmed Storage protein profile and amino acid content in wild rice Oryza glumaepatula
title_sort Storage protein profile and amino acid content in wild rice Oryza glumaepatula
author Santos, Karina Freire D’eça Nogueira
author_facet Santos, Karina Freire D’eça Nogueira
Silveira, Ricardo Diógenes Dias
Martin-Didonet, Claudia Cristina Garcia
Brondani, Claudio
author_role author
author2 Silveira, Ricardo Diógenes Dias
Martin-Didonet, Claudia Cristina Garcia
Brondani, Claudio
author2_role author
author
author
dc.contributor.none.fl_str_mv CAPES and CNPq
dc.contributor.author.fl_str_mv Santos, Karina Freire D’eça Nogueira
Silveira, Ricardo Diógenes Dias
Martin-Didonet, Claudia Cristina Garcia
Brondani, Claudio
dc.subject.por.fl_str_mv Oryza sativa; genetic resources; nutritional quality; plant breeding; SDS‑Page.
Oryza sativa; recursos genéticos; qualidade nutricional; melhoramento de plantas; SDS‑Page.
topic Oryza sativa; genetic resources; nutritional quality; plant breeding; SDS‑Page.
Oryza sativa; recursos genéticos; qualidade nutricional; melhoramento de plantas; SDS‑Page.
description The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan) in comparison to the two analized rice cultivars. The albumin and glutelin profiles in SDS-Page were distinct between rice cultivars and O. glumaepatula. This wild species has the potential to increase the nutritional quality of rice storage protein through interspecific crosses.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-18
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/12784
url https://seer.sct.embrapa.br/index.php/pab/article/view/12784
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/12784/8970
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12784/8486
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12784/8487
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12784/8488
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12784/8489
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.48, n.1, jan. 2013; 66-72
Pesquisa Agropecuária Brasileira; v.48, n.1, jan. 2013; 66-72
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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