In vitro organogenesis in watermelon cotyledons

Detalhes bibliográficos
Autor(a) principal: Krug, Maria Graziela Zagatto
Data de Publicação: 2005
Outros Autores: Stipp, Liliane Cristina Libório, Rodriguez, Adriana Pinheiro Martinelli, Mendes, Beatriz Mendes Madalena Januzzi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7037
Resumo: The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L-l) and coconut water (10%). The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants. Adventitious shoots were characterized by the development of shoot apical meristem and leaf primordia. The formation of protuberances, that do not develop into adventitious buds, was also observed.
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spelling In vitro organogenesis in watermelon cotyledonsOrganogênese in vitro em cotilédones de melanciaCitrullus lanatus; histological study; adventitious budCitrullus lanatus; estudo histológico; gema adventícia The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L-l) and coconut water (10%). The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants. Adventitious shoots were characterized by the development of shoot apical meristem and leaf primordia. The formation of protuberances, that do not develop into adventitious buds, was also observed.O objetivo do trabalho foi estudar a organogênese in vitro de C. lanatus, pela indução de gemas adventícias, em segmentos de cotilédones, cultivados em meio de cultura suplementado com citocinina. Os explantes consistiram de segmentos das regiões distal e proximal de cotilédones, coletados de plantas germinadas in vitro com um, três e cinco dias de idade. Os dados obtidos mostram que a organogênese de melancia, in vitro, ocorre com maior eficiência em segmentos da região proximal dos cotilédones, coletados de plântulas com três dias de idade e cultivados em meio de cultura MS, suplementado com a combinação BAP (1 mg L-l) e água de coco (10%). Pelo estudo histológico, verificou-se que a organogênese ocorre diretamente, sem a formação de calo, na epiderme e subepiderme do explante. As gemas adventícias foram caracterizadas pela presença de meristema apical e primórdios foliares. Observou-se, também, o desenvolvimento de protuberâncias que não se desenvolvem em gemas adventícias.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraKrug, Maria Graziela ZagattoStipp, Liliane Cristina LibórioRodriguez, Adriana Pinheiro MartinelliMendes, Beatriz Mendes Madalena Januzzi2005-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7037Pesquisa Agropecuaria Brasileira; v.40, n.9, set. 2005; 861-865Pesquisa Agropecuária Brasileira; v.40, n.9, set. 2005; 861-8651678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/7037/4085info:eu-repo/semantics/openAccess2014-09-19T19:43:53Zoai:ojs.seer.sct.embrapa.br:article/7037Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-19T19:43:53Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro organogenesis in watermelon cotyledons
Organogênese in vitro em cotilédones de melancia
title In vitro organogenesis in watermelon cotyledons
spellingShingle In vitro organogenesis in watermelon cotyledons
Krug, Maria Graziela Zagatto
Citrullus lanatus; histological study; adventitious bud
Citrullus lanatus; estudo histológico; gema adventícia
title_short In vitro organogenesis in watermelon cotyledons
title_full In vitro organogenesis in watermelon cotyledons
title_fullStr In vitro organogenesis in watermelon cotyledons
title_full_unstemmed In vitro organogenesis in watermelon cotyledons
title_sort In vitro organogenesis in watermelon cotyledons
author Krug, Maria Graziela Zagatto
author_facet Krug, Maria Graziela Zagatto
Stipp, Liliane Cristina Libório
Rodriguez, Adriana Pinheiro Martinelli
Mendes, Beatriz Mendes Madalena Januzzi
author_role author
author2 Stipp, Liliane Cristina Libório
Rodriguez, Adriana Pinheiro Martinelli
Mendes, Beatriz Mendes Madalena Januzzi
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Krug, Maria Graziela Zagatto
Stipp, Liliane Cristina Libório
Rodriguez, Adriana Pinheiro Martinelli
Mendes, Beatriz Mendes Madalena Januzzi
dc.subject.por.fl_str_mv Citrullus lanatus; histological study; adventitious bud
Citrullus lanatus; estudo histológico; gema adventícia
topic Citrullus lanatus; histological study; adventitious bud
Citrullus lanatus; estudo histológico; gema adventícia
description The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L-l) and coconut water (10%). The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants. Adventitious shoots were characterized by the development of shoot apical meristem and leaf primordia. The formation of protuberances, that do not develop into adventitious buds, was also observed.
publishDate 2005
dc.date.none.fl_str_mv 2005-09-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7037
url https://seer.sct.embrapa.br/index.php/pab/article/view/7037
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7037/4085
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.40, n.9, set. 2005; 861-865
Pesquisa Agropecuária Brasileira; v.40, n.9, set. 2005; 861-865
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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