Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/12750 |
Resumo: | The objective of this work was to characterize the oleoresin of copaiba (Copaifera reticulata) and to estimate genetic variability of the species in the Tapajós National Forest, PA, Brazil, using microsatellite markers. Sampling was performed in two areas, 5 km apart, in 136 trees. Genetic diversity was evaluated with six microsatellite markers derived from C. langsdorffii, and the oleoresin obtained from 30 trees (15 from each area) was physically and chemically characterized. Oleoresin from C. reticulate has a liquid, thin aspect, with a weak odor and yellowish‑gilded color (73.3% of the plants), highly variable viscosity (18 to 187 Pa-s), and mean density of 0,975±0,049 g cm-3. Its acidity index varied from 9.62 to 10.17 mg g-1 of KOH and the saponification index from 100.63 to 109.84 mg g-1. The molecular analysis identified 78 alleles, with an average of 13 per locus. The expected heterozygosity varied from 0.59 to 0.85 (mean of 0.75) and the inbreeding level, from 0.375 to 0.419. The genetic differentiation between populations in the different sampling areas was low (FST = 0.030), but the variability was higher between the genetic groups detected by Structure software (FST = 0.070). This higher variability indicates that the genetic diversity of copaiba is not threatened in the mid‑term. |
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Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, BrazilAnálise físico‑química do óleo‑resina e variabilidade genética de copaíba na Floresta Nacional do TapajósCopaifera reticulata; in situ conservation; inbreeding; genetic structure; extractive management; physicochemical variabilityCopaifera reticulata; conservação in situ; endogamia; estrutura genética; manejo extrativista; variabilidade físico‑químicaThe objective of this work was to characterize the oleoresin of copaiba (Copaifera reticulata) and to estimate genetic variability of the species in the Tapajós National Forest, PA, Brazil, using microsatellite markers. Sampling was performed in two areas, 5 km apart, in 136 trees. Genetic diversity was evaluated with six microsatellite markers derived from C. langsdorffii, and the oleoresin obtained from 30 trees (15 from each area) was physically and chemically characterized. Oleoresin from C. reticulate has a liquid, thin aspect, with a weak odor and yellowish‑gilded color (73.3% of the plants), highly variable viscosity (18 to 187 Pa-s), and mean density of 0,975±0,049 g cm-3. Its acidity index varied from 9.62 to 10.17 mg g-1 of KOH and the saponification index from 100.63 to 109.84 mg g-1. The molecular analysis identified 78 alleles, with an average of 13 per locus. The expected heterozygosity varied from 0.59 to 0.85 (mean of 0.75) and the inbreeding level, from 0.375 to 0.419. The genetic differentiation between populations in the different sampling areas was low (FST = 0.030), but the variability was higher between the genetic groups detected by Structure software (FST = 0.070). This higher variability indicates that the genetic diversity of copaiba is not threatened in the mid‑term.O objetivo deste trabalho foi caracterizar o óleo‑resina da copaíba (Copaifera reticulata) e estimar, por meio de marcadores microssatélites, a variabilidade genética da espécie na Floresta Nacional do Tapajós, PA. A amostragem foi realizada em duas áreas, distanciadas de 5 km, em 136 árvores. A diversidade genética foi avaliada com seis marcadores microssatélites derivados de C. langsdorffii, e o óleo obtido de 30 árvores (15 de cada área) foi caracterizado em termos físicos e químicos. O óleo C. reticulata apresenta aspecto líquido, fino, odor fraco e de coloração amarelo‑dourada (73,3% das plantas), com viscosidade muito variável (18 a 187 Pa‑s) e densidade média de 0,975±0,049 g cm-3. O índice de acidez variou de 9,62 a 10,17 mg g-1 de KOH e o de saponificação de 100,63 a 109,84 mg g-1. A análise molecular identificou 78 alelos, com média de 13 por loco. A heterozigosidade esperada variou 0,59 a 0,85 (média de 0,75), com nível de endogamia de 0,375 a 0,419. Houve pouca diferenciação genética entre as populações das diferentes áreas de coleta (FST = 0,030), mas a variabilidade foi maior entre os grupos genéticos detectados pelo programa Structure (FST = 0,070). Essa maior variabilidade indica que não há ameaças à conservação genética da copaíba, em médio prazo.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraSilva, Ederly SantosMathias, Caroline de SouzaLima, Milena Campelo Freitas deVeiga Junior, Valdir Florêncio daRodrigues, Doriane PicançoClement, Charles Roland2012-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/12750Pesquisa Agropecuaria Brasileira; v.47, n.11, nov. 2012; 1621-1628Pesquisa Agropecuária Brasileira; v.47, n.11, nov. 2012; 1621-16281678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/12750/8230https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12750/8449info:eu-repo/semantics/openAccess2012-12-19T18:13:57Zoai:ojs.seer.sct.embrapa.br:article/12750Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-12-19T18:13:57Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil Análise físico‑química do óleo‑resina e variabilidade genética de copaíba na Floresta Nacional do Tapajós |
title |
Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil |
spellingShingle |
Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil Silva, Ederly Santos Copaifera reticulata; in situ conservation; inbreeding; genetic structure; extractive management; physicochemical variability Copaifera reticulata; conservação in situ; endogamia; estrutura genética; manejo extrativista; variabilidade físico‑química |
title_short |
Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil |
title_full |
Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil |
title_fullStr |
Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil |
title_full_unstemmed |
Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil |
title_sort |
Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil |
author |
Silva, Ederly Santos |
author_facet |
Silva, Ederly Santos Mathias, Caroline de Souza Lima, Milena Campelo Freitas de Veiga Junior, Valdir Florêncio da Rodrigues, Doriane Picanço Clement, Charles Roland |
author_role |
author |
author2 |
Mathias, Caroline de Souza Lima, Milena Campelo Freitas de Veiga Junior, Valdir Florêncio da Rodrigues, Doriane Picanço Clement, Charles Roland |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Silva, Ederly Santos Mathias, Caroline de Souza Lima, Milena Campelo Freitas de Veiga Junior, Valdir Florêncio da Rodrigues, Doriane Picanço Clement, Charles Roland |
dc.subject.por.fl_str_mv |
Copaifera reticulata; in situ conservation; inbreeding; genetic structure; extractive management; physicochemical variability Copaifera reticulata; conservação in situ; endogamia; estrutura genética; manejo extrativista; variabilidade físico‑química |
topic |
Copaifera reticulata; in situ conservation; inbreeding; genetic structure; extractive management; physicochemical variability Copaifera reticulata; conservação in situ; endogamia; estrutura genética; manejo extrativista; variabilidade físico‑química |
description |
The objective of this work was to characterize the oleoresin of copaiba (Copaifera reticulata) and to estimate genetic variability of the species in the Tapajós National Forest, PA, Brazil, using microsatellite markers. Sampling was performed in two areas, 5 km apart, in 136 trees. Genetic diversity was evaluated with six microsatellite markers derived from C. langsdorffii, and the oleoresin obtained from 30 trees (15 from each area) was physically and chemically characterized. Oleoresin from C. reticulate has a liquid, thin aspect, with a weak odor and yellowish‑gilded color (73.3% of the plants), highly variable viscosity (18 to 187 Pa-s), and mean density of 0,975±0,049 g cm-3. Its acidity index varied from 9.62 to 10.17 mg g-1 of KOH and the saponification index from 100.63 to 109.84 mg g-1. The molecular analysis identified 78 alleles, with an average of 13 per locus. The expected heterozygosity varied from 0.59 to 0.85 (mean of 0.75) and the inbreeding level, from 0.375 to 0.419. The genetic differentiation between populations in the different sampling areas was low (FST = 0.030), but the variability was higher between the genetic groups detected by Structure software (FST = 0.070). This higher variability indicates that the genetic diversity of copaiba is not threatened in the mid‑term. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-17 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/12750 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/12750 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/12750/8230 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12750/8449 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.47, n.11, nov. 2012; 1621-1628 Pesquisa Agropecuária Brasileira; v.47, n.11, nov. 2012; 1621-1628 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416699648671744 |