Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, Ederly Santos
Data de Publicação: 2012
Outros Autores: Mathias, Caroline de Souza, Lima, Milena Campelo Freitas de, Veiga Junior, Valdir Florêncio da, Rodrigues, Doriane Picanço, Clement, Charles Roland
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/12750
Resumo: The objective of this work was to characterize the oleoresin of copaiba (Copaifera reticulata) and to estimate genetic variability of the species in the Tapajós National Forest, PA, Brazil, using microsatellite markers. Sampling was performed in two areas, 5 km apart, in 136 trees. Genetic diversity was evaluated with six microsatellite markers derived from C. langsdorffii, and the oleoresin obtained from 30 trees (15 from each area) was physically and chemically characterized. Oleoresin from C. reticulate has a liquid, thin aspect, with a weak odor and yellowish‑gilded color (73.3% of the plants), highly variable viscosity (18 to 187 Pa-s), and mean density of 0,975±0,049 g cm-3. Its acidity index varied from 9.62 to 10.17 mg g-1 of KOH and the saponification index from 100.63 to 109.84 mg g-1. The molecular analysis identified 78 alleles, with an average of 13 per locus. The expected heterozygosity varied from 0.59 to 0.85 (mean of 0.75) and the inbreeding level, from 0.375 to 0.419. The genetic differentiation between populations in the different sampling areas was low (FST = 0.030), but the variability was higher between the genetic groups detected by Structure software (FST = 0.070). This higher variability indicates that the genetic diversity of copaiba is not threatened in the mid‑term.
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spelling Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, BrazilAnálise físico‑química do óleo‑resina e variabilidade genética de copaíba na Floresta Nacional do TapajósCopaifera reticulata; in situ conservation; inbreeding; genetic structure; extractive management; physicochemical variabilityCopaifera reticulata; conservação in situ; endogamia; estrutura genética; manejo extrativista; variabilidade físico‑químicaThe objective of this work was to characterize the oleoresin of copaiba (Copaifera reticulata) and to estimate genetic variability of the species in the Tapajós National Forest, PA, Brazil, using microsatellite markers. Sampling was performed in two areas, 5 km apart, in 136 trees. Genetic diversity was evaluated with six microsatellite markers derived from C. langsdorffii, and the oleoresin obtained from 30 trees (15 from each area) was physically and chemically characterized. Oleoresin from C. reticulate has a liquid, thin aspect, with a weak odor and yellowish‑gilded color (73.3% of the plants), highly variable viscosity (18 to 187 Pa-s), and mean density of 0,975±0,049 g cm-3. Its acidity index varied from 9.62 to 10.17 mg g-1 of KOH and the saponification index from 100.63 to 109.84 mg g-1. The molecular analysis identified 78 alleles, with an average of 13 per locus. The expected heterozygosity varied from 0.59 to 0.85 (mean of 0.75) and the inbreeding level, from 0.375 to 0.419. The genetic differentiation between populations in the different sampling areas was low (FST = 0.030), but the variability was higher between the genetic groups detected by Structure software (FST = 0.070). This higher variability indicates that the genetic diversity of copaiba is not threatened in the mid‑term.O objetivo deste trabalho foi caracterizar o óleo‑resina da copaíba (Copaifera reticulata) e estimar, por meio de marcadores microssatélites, a variabilidade genética da espécie na Floresta Nacional do Tapajós,  PA. A amostragem foi realizada em duas áreas, distanciadas de 5 km, em 136 árvores. A diversidade genética foi avaliada com seis marcadores microssatélites derivados de C. langsdorffii, e o óleo obtido de 30 árvores (15 de cada área) foi caracterizado em termos físicos e químicos. O óleo C. reticulata apresenta aspecto líquido, fino, odor fraco e de coloração amarelo‑dourada (73,3% das plantas), com viscosidade muito variável (18 a 187 Pa‑s) e densidade média de 0,975±0,049 g cm-3. O índice de acidez variou de 9,62 a 10,17 mg g-1 de KOH e o de saponificação de 100,63 a 109,84 mg g-1. A análise molecular identificou 78 alelos, com média de 13 por loco. A heterozigosidade esperada variou 0,59 a 0,85 (média de 0,75), com nível de endogamia de 0,375 a 0,419. Houve pouca diferenciação genética entre as populações das diferentes áreas de coleta (FST = 0,030), mas a variabilidade foi maior entre os grupos genéticos detectados pelo programa Structure (FST = 0,070). Essa maior variabilidade indica que não há ameaças à conservação genética da copaíba, em médio prazo.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraSilva, Ederly SantosMathias, Caroline de SouzaLima, Milena Campelo Freitas deVeiga Junior, Valdir Florêncio daRodrigues, Doriane PicançoClement, Charles Roland2012-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/12750Pesquisa Agropecuaria Brasileira; v.47, n.11, nov. 2012; 1621-1628Pesquisa Agropecuária Brasileira; v.47, n.11, nov. 2012; 1621-16281678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/12750/8230https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12750/8449info:eu-repo/semantics/openAccess2012-12-19T18:13:57Zoai:ojs.seer.sct.embrapa.br:article/12750Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-12-19T18:13:57Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil
Análise físico‑química do óleo‑resina e variabilidade genética de copaíba na Floresta Nacional do Tapajós
title Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil
spellingShingle Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil
Silva, Ederly Santos
Copaifera reticulata; in situ conservation; inbreeding; genetic structure; extractive management; physicochemical variability
Copaifera reticulata; conservação in situ; endogamia; estrutura genética; manejo extrativista; variabilidade físico‑química
title_short Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil
title_full Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil
title_fullStr Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil
title_full_unstemmed Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil
title_sort Physico‑chemical analysis of the oleoresin and genetic variability of copaiba in the Tapajós National Forest, Brazil
author Silva, Ederly Santos
author_facet Silva, Ederly Santos
Mathias, Caroline de Souza
Lima, Milena Campelo Freitas de
Veiga Junior, Valdir Florêncio da
Rodrigues, Doriane Picanço
Clement, Charles Roland
author_role author
author2 Mathias, Caroline de Souza
Lima, Milena Campelo Freitas de
Veiga Junior, Valdir Florêncio da
Rodrigues, Doriane Picanço
Clement, Charles Roland
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Silva, Ederly Santos
Mathias, Caroline de Souza
Lima, Milena Campelo Freitas de
Veiga Junior, Valdir Florêncio da
Rodrigues, Doriane Picanço
Clement, Charles Roland
dc.subject.por.fl_str_mv Copaifera reticulata; in situ conservation; inbreeding; genetic structure; extractive management; physicochemical variability
Copaifera reticulata; conservação in situ; endogamia; estrutura genética; manejo extrativista; variabilidade físico‑química
topic Copaifera reticulata; in situ conservation; inbreeding; genetic structure; extractive management; physicochemical variability
Copaifera reticulata; conservação in situ; endogamia; estrutura genética; manejo extrativista; variabilidade físico‑química
description The objective of this work was to characterize the oleoresin of copaiba (Copaifera reticulata) and to estimate genetic variability of the species in the Tapajós National Forest, PA, Brazil, using microsatellite markers. Sampling was performed in two areas, 5 km apart, in 136 trees. Genetic diversity was evaluated with six microsatellite markers derived from C. langsdorffii, and the oleoresin obtained from 30 trees (15 from each area) was physically and chemically characterized. Oleoresin from C. reticulate has a liquid, thin aspect, with a weak odor and yellowish‑gilded color (73.3% of the plants), highly variable viscosity (18 to 187 Pa-s), and mean density of 0,975±0,049 g cm-3. Its acidity index varied from 9.62 to 10.17 mg g-1 of KOH and the saponification index from 100.63 to 109.84 mg g-1. The molecular analysis identified 78 alleles, with an average of 13 per locus. The expected heterozygosity varied from 0.59 to 0.85 (mean of 0.75) and the inbreeding level, from 0.375 to 0.419. The genetic differentiation between populations in the different sampling areas was low (FST = 0.030), but the variability was higher between the genetic groups detected by Structure software (FST = 0.070). This higher variability indicates that the genetic diversity of copaiba is not threatened in the mid‑term.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-17
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/12750
url https://seer.sct.embrapa.br/index.php/pab/article/view/12750
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/12750/8230
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12750/8449
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.47, n.11, nov. 2012; 1621-1628
Pesquisa Agropecuária Brasileira; v.47, n.11, nov. 2012; 1621-1628
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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