Methodology for measuring N2 fixation in detached roots of tropical forage grasses

Detalhes bibliográficos
Autor(a) principal: Souto, Sebastião M.
Data de Publicação: 2014
Outros Autores: Döbereiner, Johanna
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/15599
Resumo: Seven laboratory experiments were carried out to develop a new technique for the study of the nitroganase activity (C2H2 reduction) of detached roots of several tropical grasses. The effect of duration of incubation, pO2, multiplication of N2-fixing bacteria, type and periods of sampling were compared. The new method consists of harvesting pieces of roots attached to 10 cm shoots into 3 litres flask full of water, than replacing the water with N2, 3% O2 and 12% C2H2, incubating at room temperature and determining C2H2 reduction rates between 6 and 8 h. The new methodology developed to study nitrogenase activity in 4 of the 5 grasses understimated that obtained in intact soil-plant systems, but the techniques were significantly correlated with each other (r = 0,91**).
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spelling Methodology for measuring N2 fixation in detached roots of tropical forage grassesMetodologia para medição da fixação de N2 em raízes de gramíneas forrageiras tropicaisnitrogenase; duration of incubation; N2 fixing bacterianitrogenase; duração de incubação; bactérias fixadoras de N2Seven laboratory experiments were carried out to develop a new technique for the study of the nitroganase activity (C2H2 reduction) of detached roots of several tropical grasses. The effect of duration of incubation, pO2, multiplication of N2-fixing bacteria, type and periods of sampling were compared. The new method consists of harvesting pieces of roots attached to 10 cm shoots into 3 litres flask full of water, than replacing the water with N2, 3% O2 and 12% C2H2, incubating at room temperature and determining C2H2 reduction rates between 6 and 8 h. The new methodology developed to study nitrogenase activity in 4 of the 5 grasses understimated that obtained in intact soil-plant systems, but the techniques were significantly correlated with each other (r = 0,91**).Foram desenvolvidos, em laboratório, sete experimentos com o fim de desenvolver nova técnica de estudar a atividade nitrogenase (redução de C2H2) de raízes destacadas de várias gramíneas tropicais. Foram comparados o efeito da duração da incubação, pO2, multiplicação de bactérias fixadoras de N2, períodos de amostragem e tipos de amostras. O novo método consiste em medir a redução C2H2 em raízes destacadas, com 10 cm de parte aérea e incubadas em frascos de três litros cheios de água, substituindo-se, depois, a água por N2, 3% de O2 e 12% de C2H2. Nesses fracos, podem ser medidas taxas de redução de acetileno com 6 a 8 horas de incubação. Esta nova técnica, desenvolvida para estudar a atividade nitrogenase, subestimou, em 4 das 5 gramíneas, aquela encontrada para o sistema solo-planta intactos; contudo, as duas técnicas se correlacionaram significativamente (r = 0,91**).Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraSouto, Sebastião M.Döbereiner, Johanna2014-04-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/15599Pesquisa Agropecuaria Brasileira; v.19, n.5, maio 1984; 553-565Pesquisa Agropecuária Brasileira; v.19, n.5, maio 1984; 553-5651678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/15599/9604info:eu-repo/semantics/openAccess2014-04-16T19:22:17Zoai:ojs.seer.sct.embrapa.br:article/15599Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-16T19:22:17Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Methodology for measuring N2 fixation in detached roots of tropical forage grasses
Metodologia para medição da fixação de N2 em raízes de gramíneas forrageiras tropicais
title Methodology for measuring N2 fixation in detached roots of tropical forage grasses
spellingShingle Methodology for measuring N2 fixation in detached roots of tropical forage grasses
Souto, Sebastião M.
nitrogenase; duration of incubation; N2 fixing bacteria
nitrogenase; duração de incubação; bactérias fixadoras de N2
title_short Methodology for measuring N2 fixation in detached roots of tropical forage grasses
title_full Methodology for measuring N2 fixation in detached roots of tropical forage grasses
title_fullStr Methodology for measuring N2 fixation in detached roots of tropical forage grasses
title_full_unstemmed Methodology for measuring N2 fixation in detached roots of tropical forage grasses
title_sort Methodology for measuring N2 fixation in detached roots of tropical forage grasses
author Souto, Sebastião M.
author_facet Souto, Sebastião M.
Döbereiner, Johanna
author_role author
author2 Döbereiner, Johanna
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Souto, Sebastião M.
Döbereiner, Johanna
dc.subject.por.fl_str_mv nitrogenase; duration of incubation; N2 fixing bacteria
nitrogenase; duração de incubação; bactérias fixadoras de N2
topic nitrogenase; duration of incubation; N2 fixing bacteria
nitrogenase; duração de incubação; bactérias fixadoras de N2
description Seven laboratory experiments were carried out to develop a new technique for the study of the nitroganase activity (C2H2 reduction) of detached roots of several tropical grasses. The effect of duration of incubation, pO2, multiplication of N2-fixing bacteria, type and periods of sampling were compared. The new method consists of harvesting pieces of roots attached to 10 cm shoots into 3 litres flask full of water, than replacing the water with N2, 3% O2 and 12% C2H2, incubating at room temperature and determining C2H2 reduction rates between 6 and 8 h. The new methodology developed to study nitrogenase activity in 4 of the 5 grasses understimated that obtained in intact soil-plant systems, but the techniques were significantly correlated with each other (r = 0,91**).
publishDate 2014
dc.date.none.fl_str_mv 2014-04-16
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/15599
url https://seer.sct.embrapa.br/index.php/pab/article/view/15599
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/15599/9604
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.19, n.5, maio 1984; 553-565
Pesquisa Agropecuária Brasileira; v.19, n.5, maio 1984; 553-565
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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