Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato
Autor(a) principal: | |
---|---|
Data de Publicação: | 2000 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/5950 |
Resumo: | The objective of this work was to evaluate the regeneration capacity of the IPA-5 andIPA-6 Brazilian industrial tomato (Lycopersicon esculentum Mill) cultivars using four compositions ofculture media described in the literature and five inoculation methods. A new variation of inoculation,the split cotyledon method, was also tested. The largest frequency of shoot bud formation was 100%in the case of IPA-5 and 65% in the case of IPA-6. To induce shoot elongation, it was necessary toaccomplish three subcultures of the explants presenting shoot buds. In the case of IPA-5, the numberof the obtained shoots was higher when the induction of shoot buds was accomplished in culturemedium containing BAP (2.5 mg L-1) and IAA (0.2 mg L-1) followed by three subcultures on zeatin(0.5 mg -1) containing medium. Using such protocol, the IPA-5 cultivar produced, on the average, 5.45elongated shoots through the split cotyledon method. That capacity exceeded significantly the trimmedcotyledon method, which produced 4.4 elongated shoots per explant. In the case of IPA-6, the bestcombination of culture medium and inoculation method produced 0.87 elongated shoot per explant.The elongated shoots were rooted and transferred to greenhouse. |
id |
EMBRAPA-4_162531c183be18b2c65368fd0564fbd9 |
---|---|
oai_identifier_str |
oai:ojs.seer.sct.embrapa.br:article/5950 |
network_acronym_str |
EMBRAPA-4 |
network_name_str |
Pesquisa Agropecuária Brasileira (Online) |
repository_id_str |
|
spelling |
Evaluation of the in vitro regeneration capacity in Brazilian industrial tomatoAvaliação da capacidade de regeneração in vitro em tomateiro industrialLycopersicon esculentum; cultivars; organogenesis; tissue culture; cotyledons; explants; genetic transformationLycopersicon esculentum; cultivares; organogênese; cultura de tecidos; cotilédone; explante; transformação genéticaThe objective of this work was to evaluate the regeneration capacity of the IPA-5 andIPA-6 Brazilian industrial tomato (Lycopersicon esculentum Mill) cultivars using four compositions ofculture media described in the literature and five inoculation methods. A new variation of inoculation,the split cotyledon method, was also tested. The largest frequency of shoot bud formation was 100%in the case of IPA-5 and 65% in the case of IPA-6. To induce shoot elongation, it was necessary toaccomplish three subcultures of the explants presenting shoot buds. In the case of IPA-5, the numberof the obtained shoots was higher when the induction of shoot buds was accomplished in culturemedium containing BAP (2.5 mg L-1) and IAA (0.2 mg L-1) followed by three subcultures on zeatin(0.5 mg -1) containing medium. Using such protocol, the IPA-5 cultivar produced, on the average, 5.45elongated shoots through the split cotyledon method. That capacity exceeded significantly the trimmedcotyledon method, which produced 4.4 elongated shoots per explant. In the case of IPA-6, the bestcombination of culture medium and inoculation method produced 0.87 elongated shoot per explant.The elongated shoots were rooted and transferred to greenhouse.Este trabalho teve por objetivo avaliar a capacidade de regeneração das cultivares detomateiro industrial (Lycopersicon esculentum Mill) IPA-5 e IPA-6, utilizando quatro composições demeio de cultura descritos na literatura e cinco variações de inoculação. Foi testada uma nova variação deinoculação, denominada cotilédone fendido. A maior freqüência de formação de gemas vegetativas foi100% no caso de IPA-5, e 65% no caso de IPA-6. Para induzir o alongamento de brotos, foramnecessários três subcultivos dos explantes apresentando gemas. No caso de IPA-5, o número de brotosobtidos foi maior quando a indução de gemas foi realizada em meio contendo BAP (2,5 mg L-1) eAIA (0,2 mg L-1) seguido de três subcultivos, em meio como zeatina (0,5 mg L-1). Usando esse protocolo,a cultivar IPA-5 produziu uma média de 5,45 brotos alongados a partir do cotilédone fendido. Essacapacidade excedeu significativamente o cotilédone aparado, que produziu 4,4 brotos alongados porexplante. No caso de IPA-6, a melhor combinação de meios e método de inoculação produziu0,87 broto alongado por explante. Os brotos alongados foram enraizados e transferidos para casa devegetação.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFári, MiklósResende, Geraldo Milanez deMelo, Natoniel Franklin de2000-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/5950Pesquisa Agropecuaria Brasileira; v.35, n.8, ago. 2000; 1523-1529Pesquisa Agropecuária Brasileira; v.35, n.8, ago. 2000; 1523-15291678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/5950/3054info:eu-repo/semantics/openAccess2014-08-19T19:06:48Zoai:ojs.seer.sct.embrapa.br:article/5950Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-19T19:06:48Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato Avaliação da capacidade de regeneração in vitro em tomateiro industrial |
title |
Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato |
spellingShingle |
Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato Fári, Miklós Lycopersicon esculentum; cultivars; organogenesis; tissue culture; cotyledons; explants; genetic transformation Lycopersicon esculentum; cultivares; organogênese; cultura de tecidos; cotilédone; explante; transformação genética |
title_short |
Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato |
title_full |
Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato |
title_fullStr |
Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato |
title_full_unstemmed |
Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato |
title_sort |
Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato |
author |
Fári, Miklós |
author_facet |
Fári, Miklós Resende, Geraldo Milanez de Melo, Natoniel Franklin de |
author_role |
author |
author2 |
Resende, Geraldo Milanez de Melo, Natoniel Franklin de |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Fári, Miklós Resende, Geraldo Milanez de Melo, Natoniel Franklin de |
dc.subject.por.fl_str_mv |
Lycopersicon esculentum; cultivars; organogenesis; tissue culture; cotyledons; explants; genetic transformation Lycopersicon esculentum; cultivares; organogênese; cultura de tecidos; cotilédone; explante; transformação genética |
topic |
Lycopersicon esculentum; cultivars; organogenesis; tissue culture; cotyledons; explants; genetic transformation Lycopersicon esculentum; cultivares; organogênese; cultura de tecidos; cotilédone; explante; transformação genética |
description |
The objective of this work was to evaluate the regeneration capacity of the IPA-5 andIPA-6 Brazilian industrial tomato (Lycopersicon esculentum Mill) cultivars using four compositions ofculture media described in the literature and five inoculation methods. A new variation of inoculation,the split cotyledon method, was also tested. The largest frequency of shoot bud formation was 100%in the case of IPA-5 and 65% in the case of IPA-6. To induce shoot elongation, it was necessary toaccomplish three subcultures of the explants presenting shoot buds. In the case of IPA-5, the numberof the obtained shoots was higher when the induction of shoot buds was accomplished in culturemedium containing BAP (2.5 mg L-1) and IAA (0.2 mg L-1) followed by three subcultures on zeatin(0.5 mg -1) containing medium. Using such protocol, the IPA-5 cultivar produced, on the average, 5.45elongated shoots through the split cotyledon method. That capacity exceeded significantly the trimmedcotyledon method, which produced 4.4 elongated shoots per explant. In the case of IPA-6, the bestcombination of culture medium and inoculation method produced 0.87 elongated shoot per explant.The elongated shoots were rooted and transferred to greenhouse. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-08-01 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/5950 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/5950 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/5950/3054 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.35, n.8, ago. 2000; 1523-1529 Pesquisa Agropecuária Brasileira; v.35, n.8, ago. 2000; 1523-1529 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
_version_ |
1793416711271088128 |