Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato

Detalhes bibliográficos
Autor(a) principal: Fári, Miklós
Data de Publicação: 2000
Outros Autores: Resende, Geraldo Milanez de, Melo, Natoniel Franklin de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/5950
Resumo: The objective of this work was to evaluate the regeneration capacity of the IPA-5 andIPA-6 Brazilian industrial tomato (Lycopersicon esculentum Mill) cultivars using four compositions ofculture media described in the literature and five inoculation methods. A new variation of inoculation,the split cotyledon method, was also tested. The largest frequency of shoot bud formation was 100%in the case of IPA-5 and 65% in the case of IPA-6. To induce shoot elongation, it was necessary toaccomplish three subcultures of the explants presenting shoot buds. In the case of IPA-5, the numberof the obtained shoots was higher when the induction of shoot buds was accomplished in culturemedium containing BAP (2.5 mg L-1) and IAA (0.2 mg L-1) followed by three subcultures on zeatin(0.5 mg -1) containing medium. Using such protocol, the IPA-5 cultivar produced, on the average, 5.45elongated shoots through the split cotyledon method. That capacity exceeded significantly the trimmedcotyledon method, which produced 4.4 elongated shoots per explant. In the case of IPA-6, the bestcombination of culture medium and inoculation method produced 0.87 elongated shoot per explant.The elongated shoots were rooted and transferred to greenhouse.
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spelling Evaluation of the in vitro regeneration capacity in Brazilian industrial tomatoAvaliação da capacidade de regeneração in vitro em tomateiro industrialLycopersicon esculentum; cultivars; organogenesis; tissue culture; cotyledons; explants; genetic transformationLycopersicon esculentum; cultivares; organogênese; cultura de tecidos; cotilédone; explante; transformação genéticaThe objective of this work was to evaluate the regeneration capacity of the IPA-5 andIPA-6 Brazilian industrial tomato (Lycopersicon esculentum Mill) cultivars using four compositions ofculture media described in the literature and five inoculation methods. A new variation of inoculation,the split cotyledon method, was also tested. The largest frequency of shoot bud formation was 100%in the case of IPA-5 and 65% in the case of IPA-6. To induce shoot elongation, it was necessary toaccomplish three subcultures of the explants presenting shoot buds. In the case of IPA-5, the numberof the obtained shoots was higher when the induction of shoot buds was accomplished in culturemedium containing BAP (2.5 mg L-1) and IAA (0.2 mg L-1) followed by three subcultures on zeatin(0.5 mg -1) containing medium. Using such protocol, the IPA-5 cultivar produced, on the average, 5.45elongated shoots through the split cotyledon method. That capacity exceeded significantly the trimmedcotyledon method, which produced 4.4 elongated shoots per explant. In the case of IPA-6, the bestcombination of culture medium and inoculation method produced 0.87 elongated shoot per explant.The elongated shoots were rooted and transferred to greenhouse.Este trabalho teve por objetivo avaliar a capacidade de regeneração das cultivares detomateiro industrial (Lycopersicon esculentum Mill) IPA-5 e IPA-6, utilizando quatro composições demeio de cultura descritos na literatura e cinco variações de inoculação. Foi testada uma nova variação deinoculação, denominada cotilédone fendido. A maior freqüência de formação de gemas vegetativas foi100% no caso de IPA-5, e 65% no caso de IPA-6. Para induzir o alongamento de brotos, foramnecessários três subcultivos dos explantes apresentando gemas. No caso de IPA-5, o número de brotosobtidos foi maior quando a indução de gemas foi realizada em meio contendo BAP (2,5 mg L-1) eAIA (0,2 mg L-1) seguido de três subcultivos, em meio como zeatina (0,5 mg L-1). Usando esse protocolo,a cultivar IPA-5 produziu uma média de 5,45 brotos alongados a partir do cotilédone fendido. Essacapacidade excedeu significativamente o cotilédone aparado, que produziu 4,4 brotos alongados porexplante. No caso de IPA-6, a melhor combinação de meios e método de inoculação produziu0,87 broto alongado por explante. Os brotos alongados foram enraizados e transferidos para casa devegetação.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFári, MiklósResende, Geraldo Milanez deMelo, Natoniel Franklin de2000-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/5950Pesquisa Agropecuaria Brasileira; v.35, n.8, ago. 2000; 1523-1529Pesquisa Agropecuária Brasileira; v.35, n.8, ago. 2000; 1523-15291678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/5950/3054info:eu-repo/semantics/openAccess2014-08-19T19:06:48Zoai:ojs.seer.sct.embrapa.br:article/5950Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-19T19:06:48Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato
Avaliação da capacidade de regeneração in vitro em tomateiro industrial
title Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato
spellingShingle Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato
Fári, Miklós
Lycopersicon esculentum; cultivars; organogenesis; tissue culture; cotyledons; explants; genetic transformation
Lycopersicon esculentum; cultivares; organogênese; cultura de tecidos; cotilédone; explante; transformação genética
title_short Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato
title_full Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato
title_fullStr Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato
title_full_unstemmed Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato
title_sort Evaluation of the in vitro regeneration capacity in Brazilian industrial tomato
author Fári, Miklós
author_facet Fári, Miklós
Resende, Geraldo Milanez de
Melo, Natoniel Franklin de
author_role author
author2 Resende, Geraldo Milanez de
Melo, Natoniel Franklin de
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Fári, Miklós
Resende, Geraldo Milanez de
Melo, Natoniel Franklin de
dc.subject.por.fl_str_mv Lycopersicon esculentum; cultivars; organogenesis; tissue culture; cotyledons; explants; genetic transformation
Lycopersicon esculentum; cultivares; organogênese; cultura de tecidos; cotilédone; explante; transformação genética
topic Lycopersicon esculentum; cultivars; organogenesis; tissue culture; cotyledons; explants; genetic transformation
Lycopersicon esculentum; cultivares; organogênese; cultura de tecidos; cotilédone; explante; transformação genética
description The objective of this work was to evaluate the regeneration capacity of the IPA-5 andIPA-6 Brazilian industrial tomato (Lycopersicon esculentum Mill) cultivars using four compositions ofculture media described in the literature and five inoculation methods. A new variation of inoculation,the split cotyledon method, was also tested. The largest frequency of shoot bud formation was 100%in the case of IPA-5 and 65% in the case of IPA-6. To induce shoot elongation, it was necessary toaccomplish three subcultures of the explants presenting shoot buds. In the case of IPA-5, the numberof the obtained shoots was higher when the induction of shoot buds was accomplished in culturemedium containing BAP (2.5 mg L-1) and IAA (0.2 mg L-1) followed by three subcultures on zeatin(0.5 mg -1) containing medium. Using such protocol, the IPA-5 cultivar produced, on the average, 5.45elongated shoots through the split cotyledon method. That capacity exceeded significantly the trimmedcotyledon method, which produced 4.4 elongated shoots per explant. In the case of IPA-6, the bestcombination of culture medium and inoculation method produced 0.87 elongated shoot per explant.The elongated shoots were rooted and transferred to greenhouse.
publishDate 2000
dc.date.none.fl_str_mv 2000-08-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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format article
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dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5950
url https://seer.sct.embrapa.br/index.php/pab/article/view/5950
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5950/3054
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.35, n.8, ago. 2000; 1523-1529
Pesquisa Agropecuária Brasileira; v.35, n.8, ago. 2000; 1523-1529
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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