Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1

Detalhes bibliográficos
Autor(a) principal: Filippi, Marta Cristina
Data de Publicação: 2002
Outros Autores: Prabhu, Anne Sitarama, de Araújo, Leila Garcês, de Faria, Josias Correa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6518
Resumo: Rice blast is a major yield constraint of the irrigated rice in the State of Tocantins, Brazil. The objective of this investigation was to study the phenotypic and genetic diversity within the pathogen population of Pyricularia grisea in samples collected from four individual farms of rice cultivar Metica-1, under epidemic conditions of leaf blast. A set of 87 isolates was tested on 32 rice genotypes including eight international differentials. Considering 80% similarity in virulence, two groups comprising a total of 81 isolates were recognized, independently of the farms from which they were collected. Eighty percent of the isolates pertained to pathotype ID-14, indicating high cultivar specificity and narrow diversity of virulence in the sample population. The virulence in pathogen population on rice cultivars BR-IRGA 409 and Rio Formoso was low. Analysis of P. grisea isolates using rep-PCR with two primer sequences from Pot2 generated fingerprint profiles of one to nine bands. Cluster analysis revealed the occurrence of six fingerprint groups with similarities ranging from 0.09 to 1. There was no straight relationship between virulence of the isolates based on reaction pattern on 32 genotypes and grouping based on Pot2 rep-PCR analysis of P. grisea isolates collected from 'Metica-1'.
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spelling Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1Oryza sativa; pathotypes; rice blast; pathogenicityryza sativa; patótipo; brusone; patogenicidade Rice blast is a major yield constraint of the irrigated rice in the State of Tocantins, Brazil. The objective of this investigation was to study the phenotypic and genetic diversity within the pathogen population of Pyricularia grisea in samples collected from four individual farms of rice cultivar Metica-1, under epidemic conditions of leaf blast. A set of 87 isolates was tested on 32 rice genotypes including eight international differentials. Considering 80% similarity in virulence, two groups comprising a total of 81 isolates were recognized, independently of the farms from which they were collected. Eighty percent of the isolates pertained to pathotype ID-14, indicating high cultivar specificity and narrow diversity of virulence in the sample population. The virulence in pathogen population on rice cultivars BR-IRGA 409 and Rio Formoso was low. Analysis of P. grisea isolates using rep-PCR with two primer sequences from Pot2 generated fingerprint profiles of one to nine bands. Cluster analysis revealed the occurrence of six fingerprint groups with similarities ranging from 0.09 to 1. There was no straight relationship between virulence of the isolates based on reaction pattern on 32 genotypes and grouping based on Pot2 rep-PCR analysis of P. grisea isolates collected from 'Metica-1'.A brusone é um dos fatores limitantes para a produção de arroz de várzea no Estado do Tocantins. A diversidade fenotípica e genética em população de Pyricularia grisea foi estudada, em amostras coletadas em quatro lavouras da cultivar Metica-1, sob condições de epidemias de brusone nas folhas. Foram testados 87 isolados em 32 genótipos, incluindo oito diferenciadoras internacionais. Considerando 80% de similaridade em virulência, dois grupos compostos por 81 isolados foram identificados, independentemente das lavouras onde foram coletados. Oitenta por cento dos isolados pertencem ao patótipo ID-14, indicando alta especificidade da cultivar e a diversidade estreita em virulência na população estudada. A virulência da população do patógeno nas cultivares comerciais BR-IRGA 409 e Rio Formoso foi baixa. A análise de isolados de P. grisea utilizando rep-PCR com dois primers Pot2 gerou de uma a nove bandas. A análise de agrupamento revelou a ocorrência de seis grupos ou linhagens com similaridade variando de 0,09 a 1. Não houve uma relação clara entre a virulência dos isolados dos 32 genótipos e o agrupamento baseado na análise usando rep-PCR de Pot2 de isolados de P. grisea coletados na cultivar Metica-1.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFilippi, Marta CristinaPrabhu, Anne Sitaramade Araújo, Leila Garcêsde Faria, Josias Correa2002-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6518Pesquisa Agropecuaria Brasileira; v.37, n.12, dez. 2002; 1681-1688Pesquisa Agropecuária Brasileira; v.37, n.12, dez. 2002; 1681-16881678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/6518/3575info:eu-repo/semantics/openAccess2014-07-21T17:20:45Zoai:ojs.seer.sct.embrapa.br:article/6518Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-21T17:20:45Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1
Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1
title Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1
spellingShingle Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1
Filippi, Marta Cristina
Oryza sativa; pathotypes; rice blast; pathogenicity
ryza sativa; patótipo; brusone; patogenicidade
title_short Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1
title_full Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1
title_fullStr Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1
title_full_unstemmed Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1
title_sort Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1
author Filippi, Marta Cristina
author_facet Filippi, Marta Cristina
Prabhu, Anne Sitarama
de Araújo, Leila Garcês
de Faria, Josias Correa
author_role author
author2 Prabhu, Anne Sitarama
de Araújo, Leila Garcês
de Faria, Josias Correa
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Filippi, Marta Cristina
Prabhu, Anne Sitarama
de Araújo, Leila Garcês
de Faria, Josias Correa
dc.subject.por.fl_str_mv Oryza sativa; pathotypes; rice blast; pathogenicity
ryza sativa; patótipo; brusone; patogenicidade
topic Oryza sativa; pathotypes; rice blast; pathogenicity
ryza sativa; patótipo; brusone; patogenicidade
description Rice blast is a major yield constraint of the irrigated rice in the State of Tocantins, Brazil. The objective of this investigation was to study the phenotypic and genetic diversity within the pathogen population of Pyricularia grisea in samples collected from four individual farms of rice cultivar Metica-1, under epidemic conditions of leaf blast. A set of 87 isolates was tested on 32 rice genotypes including eight international differentials. Considering 80% similarity in virulence, two groups comprising a total of 81 isolates were recognized, independently of the farms from which they were collected. Eighty percent of the isolates pertained to pathotype ID-14, indicating high cultivar specificity and narrow diversity of virulence in the sample population. The virulence in pathogen population on rice cultivars BR-IRGA 409 and Rio Formoso was low. Analysis of P. grisea isolates using rep-PCR with two primer sequences from Pot2 generated fingerprint profiles of one to nine bands. Cluster analysis revealed the occurrence of six fingerprint groups with similarities ranging from 0.09 to 1. There was no straight relationship between virulence of the isolates based on reaction pattern on 32 genotypes and grouping based on Pot2 rep-PCR analysis of P. grisea isolates collected from 'Metica-1'.
publishDate 2002
dc.date.none.fl_str_mv 2002-12-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6518
url https://seer.sct.embrapa.br/index.php/pab/article/view/6518
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6518/3575
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.37, n.12, dez. 2002; 1681-1688
Pesquisa Agropecuária Brasileira; v.37, n.12, dez. 2002; 1681-1688
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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