Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia

Detalhes bibliográficos
Autor(a) principal: de Almeida, Marcílio
Data de Publicação: 2006
Outros Autores: de Almeida, Cristina Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7302
Resumo: – The aim of this work was to evaluate a protocol for plant regeneration by means of somatic embryos obtained from isolated adult pejibaye leaf primordia, and to describe histological origin of embryos and morphogenetic response. Explants were cultivated in modified MS medium. Mesophyll parenchymatous cells originated meristemoids (preembryonic complex formation) induced with 7.1 µM BAP in the first two subculture periods. After polarized structures with 12.9 µM NAA and 3.55 µM BAP were formed in the third subculture, somatic embryos developed and regenerated normal plants. The mesophyll parenchymatous cells display high capacity of direct response to the auxin and cytokinin.
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spelling Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordiaEmbriogênese somática e regeneração de plantas in vitro a partir de primórdios foliares de pupunheiras adultasBactris gasipaes; histology; micropropagation; peach palmBactris gasipaes; histologia; micropropagação– The aim of this work was to evaluate a protocol for plant regeneration by means of somatic embryos obtained from isolated adult pejibaye leaf primordia, and to describe histological origin of embryos and morphogenetic response. Explants were cultivated in modified MS medium. Mesophyll parenchymatous cells originated meristemoids (preembryonic complex formation) induced with 7.1 µM BAP in the first two subculture periods. After polarized structures with 12.9 µM NAA and 3.55 µM BAP were formed in the third subculture, somatic embryos developed and regenerated normal plants. The mesophyll parenchymatous cells display high capacity of direct response to the auxin and cytokinin.O objetivo deste trabalho foi avaliar um protocolo de regeneração de plantas por meio de embriões somáticos, obtidos a partir de primórdios foliares de pupunheiras adultas e identificar a origem histológica dos embriões e descrever as etapas morfogenéticas. Os explantes foram cultivados em meio MS modificado. Células parenquimáticas do mesofilo originaram meristemóides com BAP (7,1 µM) nos dois primeiros períodos de subcultura. A polarização das estruturas ocorreu com ANA (12,9 µM) e BAP (3,55 µM) no terceiro período de subcultura. Meristemóides se desenvolveram em embriões somáticos, regenerando plantas normais. As células parenquimáticas do mesofilo apresentam elevada capacidade de resposta direta à auxina e à citocinina.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária Brasileirade Almeida, Marcíliode Almeida, Cristina Vieira2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7302Pesquisa Agropecuaria Brasileira; v.41, n.9, set. 2006; 1449-1452Pesquisa Agropecuária Brasileira; v.41, n.9, set. 2006; 1449-14521678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/7302/4347info:eu-repo/semantics/openAccess2014-10-01T16:47:47Zoai:ojs.seer.sct.embrapa.br:article/7302Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-10-01T16:47:47Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia
Embriogênese somática e regeneração de plantas in vitro a partir de primórdios foliares de pupunheiras adultas
title Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia
spellingShingle Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia
de Almeida, Marcílio
Bactris gasipaes; histology; micropropagation; peach palm
Bactris gasipaes; histologia; micropropagação
title_short Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia
title_full Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia
title_fullStr Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia
title_full_unstemmed Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia
title_sort Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia
author de Almeida, Marcílio
author_facet de Almeida, Marcílio
de Almeida, Cristina Vieira
author_role author
author2 de Almeida, Cristina Vieira
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv de Almeida, Marcílio
de Almeida, Cristina Vieira
dc.subject.por.fl_str_mv Bactris gasipaes; histology; micropropagation; peach palm
Bactris gasipaes; histologia; micropropagação
topic Bactris gasipaes; histology; micropropagation; peach palm
Bactris gasipaes; histologia; micropropagação
description – The aim of this work was to evaluate a protocol for plant regeneration by means of somatic embryos obtained from isolated adult pejibaye leaf primordia, and to describe histological origin of embryos and morphogenetic response. Explants were cultivated in modified MS medium. Mesophyll parenchymatous cells originated meristemoids (preembryonic complex formation) induced with 7.1 µM BAP in the first two subculture periods. After polarized structures with 12.9 µM NAA and 3.55 µM BAP were formed in the third subculture, somatic embryos developed and regenerated normal plants. The mesophyll parenchymatous cells display high capacity of direct response to the auxin and cytokinin.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7302
url https://seer.sct.embrapa.br/index.php/pab/article/view/7302
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7302/4347
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.41, n.9, set. 2006; 1449-1452
Pesquisa Agropecuária Brasileira; v.41, n.9, set. 2006; 1449-1452
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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