Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm

Detalhes bibliográficos
Autor(a) principal: Carneiro, Andréa Almeida
Data de Publicação: 2008
Outros Autores: Gomes, Eliane Aparecida, Guimarães, Claudia Teixeira, Fernandes, Fernando Tavares, Carneiro, Newton Portilho, Cruz, Ivan
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/81
Resumo: The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.
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spelling Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armywormGenotipaje de aislados de Beauveria spp. por secuenciamiento de la región ITS del rDNA y marcadores RAPDCaracterização molecular e virulência de isolados de Beauveria spp. contra a lagarta-do-cartuchoSpodoptera frugiperda; entomopathogenic fungi; RAPD; rDNA-ITSSpodoptera frugiperda;fungo entomopatogênico;RAPD;rDNA-ITSThe objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.Beauveria es un hongo deuteromiceto de gran espectro entomopatogénico. Existe mucho interés en el uso de ese hongo en programas integrados de manejo de plagas, como una alternativa a los defensivos químicos. La caracterización molecular de los aislados altamente virulentos es esencial en la selección de potenciales candidatos para utilización en control biológico. Se han evaluado en este trabajo 24 aislados de Beauveria, en bioensayos contra gusanos de Spodoptera frugiperda y molecularmente caracterizados por secuenciamiento de la región ITS del rDNA y marcadores RAPD. El secuenciamiento de fragmentos de 570 pares de bases de la región ITS del rDNA posibilitó la identificación de los aislados como B. bassiana y B. brongniarti, por la comparación con secuencias depositadas en el GenBank, pero no fue suficiente para discriminar los aislados dentro de cada especie. Sin embargo, 67 fragmentos polimórficos de RAPD fueron capaces de diferenciar casi todos los aislados de Beauveria, agrupándolos de acuerdo con el insecto hospedero y la virulencia contra el gusano cogollero del maíz. Los marcadores RAPD fueron altamente asociados a la virulencia contra S. frugiperda, explicando hasta el 67% de la variación fenotípica. Por lo tanto, además de la identificación y caracterización molecular de aislados de Beauveria, el secuenciamiento de la región ITS y los marcadores RAPD fueron útiles en la selección de aislados potencialmente eficientes contra gusanos de S. frugiperda e en el monitoramiento de liberaciones de esos microrganismos, en programas de biocontrolO objetivo deste trabalho foi avaliar a patogenicidade de 24 isolados de Beauveria contra larvas de Spodoptera frugiperda e caracterizá-los molecularmente, por meio do seqüenciamento da região ITS do rDNA e de marcadores RAPD. O seqüenciamento de fragmentos de 570 pares de bases, da região ITS do rDNA, possibilitou a identificação dos isolados como B. bassiana e B. brongniarti, pela comparação com seqüências depositadas no GenBank. Sessenta e sete fragmentos polimórficos de RAPD foram capazes de diferenciar 20 entre 24 isolados de Beauveria, e agrupá-los de acordo com o inseto hospedeiro e com a patogenicidade contra a lagarta-do-cartucho do milho. Os marcadores RAPD foram altamente associados à patogenicidade contra S. frugiperda e explicaram até 67% da variação fenotípica. Além da identificação e caracterização molecular de isolados de Beauveria, o seqüenciamento da região ITS, aliado aos marcadores RAPD, é útil na seleção de isolados potencialmente eficazes contra larvas de S. frugiperda e no monitoramento de liberações desses microrganismos em programas de biocontrole.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraEmbrapaCarneiro, Andréa AlmeidaGomes, Eliane AparecidaGuimarães, Claudia TeixeiraFernandes, Fernando TavaresCarneiro, Newton PortilhoCruz, Ivan2008-10-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/81Pesquisa Agropecuaria Brasileira; v.43, n.4, abr. 2008; 513-520Pesquisa Agropecuária Brasileira; v.43, n.4, abr. 2008; 513-5201678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/81/5415https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/81/455https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/81/456info:eu-repo/semantics/openAccess2014-05-16T17:40:43Zoai:ojs.seer.sct.embrapa.br:article/81Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-05-16T17:40:43Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
Genotipaje de aislados de Beauveria spp. por secuenciamiento de la región ITS del rDNA y marcadores RAPD
Caracterização molecular e virulência de isolados de Beauveria spp. contra a lagarta-do-cartucho
title Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
spellingShingle Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
Carneiro, Andréa Almeida
Spodoptera frugiperda; entomopathogenic fungi; RAPD; rDNA-ITS
Spodoptera frugiperda;fungo entomopatogênico;RAPD;rDNA-ITS
title_short Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
title_full Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
title_fullStr Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
title_full_unstemmed Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
title_sort Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
author Carneiro, Andréa Almeida
author_facet Carneiro, Andréa Almeida
Gomes, Eliane Aparecida
Guimarães, Claudia Teixeira
Fernandes, Fernando Tavares
Carneiro, Newton Portilho
Cruz, Ivan
author_role author
author2 Gomes, Eliane Aparecida
Guimarães, Claudia Teixeira
Fernandes, Fernando Tavares
Carneiro, Newton Portilho
Cruz, Ivan
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
Embrapa
dc.contributor.author.fl_str_mv Carneiro, Andréa Almeida
Gomes, Eliane Aparecida
Guimarães, Claudia Teixeira
Fernandes, Fernando Tavares
Carneiro, Newton Portilho
Cruz, Ivan
dc.subject.por.fl_str_mv Spodoptera frugiperda; entomopathogenic fungi; RAPD; rDNA-ITS
Spodoptera frugiperda;fungo entomopatogênico;RAPD;rDNA-ITS
topic Spodoptera frugiperda; entomopathogenic fungi; RAPD; rDNA-ITS
Spodoptera frugiperda;fungo entomopatogênico;RAPD;rDNA-ITS
description The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-20
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/81
url https://seer.sct.embrapa.br/index.php/pab/article/view/81
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/81/5415
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/81/455
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/81/456
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.43, n.4, abr. 2008; 513-520
Pesquisa Agropecuária Brasileira; v.43, n.4, abr. 2008; 513-520
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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