Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States

Detalhes bibliográficos
Autor(a) principal: Botelho, Stephanie Regina Alves
Data de Publicação: 2016
Outros Autores: Duarte, Macária Ferreira, Barbosa, Andreza Viveiros, Lau, Douglas, Sanches, Márcio Martinello, Fernandes, Fernanda Rausch
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/20981
Resumo: The objective of this work was to evaluate the phytosanitary aspect of two accessions of maize (Zea mays) seeds from the United States introduced to Brazil, regarding the presence of Wheat mosaic virus (WMoV). Two to three weeks after sowing, symptomatic leaves were tested by Elisa using specific antiserum to WMoV. The reaction was positive, and leaf samples were analyzed by real‑time PCR and amplified PCR products were sequenced. The WMoV isolates had 99 to 100% nucleotide identity with isolates from Australia and the United States. Until now, there is no report of the presence of this virus in Brazil. According to the federal law on plant protection, the plants were burned to avoid the introduction of this exotic pest in the country. The obtained results show WMoV interception in Brazil.
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spelling Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United StatesInterceptação do Wheat mosaic virus (WMoV) no Brasil em sementes de milho provenientes dos Estados UnidosZea mays; exotic pest; High plains virus; plant quarantine; phytosanitary analysisZea mays; praga exótica; High plains virus; quarentena vegetal; análise fitossanitáriaThe objective of this work was to evaluate the phytosanitary aspect of two accessions of maize (Zea mays) seeds from the United States introduced to Brazil, regarding the presence of Wheat mosaic virus (WMoV). Two to three weeks after sowing, symptomatic leaves were tested by Elisa using specific antiserum to WMoV. The reaction was positive, and leaf samples were analyzed by real‑time PCR and amplified PCR products were sequenced. The WMoV isolates had 99 to 100% nucleotide identity with isolates from Australia and the United States. Until now, there is no report of the presence of this virus in Brazil. According to the federal law on plant protection, the plants were burned to avoid the introduction of this exotic pest in the country. The obtained results show WMoV interception in Brazil.O objetivo deste trabalho foi avaliar o aspecto fitossanitário de dois acessos de sementes de milho dos Estados Unidos introduzidas no Brasil, quanto à presença do Wheat mosaic virus (WMoV). Duas a três semanas após a semeadura, folhas sintomáticas foram testadas por Elisa, tendo-se utilizado antissoro específico para WMoV. A reação foi positiva, e as amostras foliares foram analisadas por PCR em tempo real e os produtos de PCR amplificados foram sequenciados. Os isolados de WMoV apresentaram 99 a 100% de identidade nucleotídica com isolados da Austrália e dos Estados Unidos. Até o momento, não há relato da presença desse vírus no Brasil. De acordo com a legislação federal de defesa vegetal, as plantas foram incineradas para evitar a introdução dessa praga exótica no País. Os resultados obtidos mostram a interceptação de WMoV no Brasil.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBotelho, Stephanie Regina AlvesDuarte, Macária FerreiraBarbosa, Andreza ViveirosLau, DouglasSanches, Márcio MartinelloFernandes, Fernanda Rausch2016-08-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/20981Pesquisa Agropecuaria Brasileira; v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária; 688-691Pesquisa Agropecuária Brasileira; v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária; 688-6911678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/20981/13336https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13137https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13138https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13139https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13140https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13141https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13142Direitos autorais 2016 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2016-08-10T14:29:22Zoai:ojs.seer.sct.embrapa.br:article/20981Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2016-08-10T14:29:22Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States
Interceptação do Wheat mosaic virus (WMoV) no Brasil em sementes de milho provenientes dos Estados Unidos
title Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States
spellingShingle Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States
Botelho, Stephanie Regina Alves
Zea mays; exotic pest; High plains virus; plant quarantine; phytosanitary analysis
Zea mays; praga exótica; High plains virus; quarentena vegetal; análise fitossanitária
title_short Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States
title_full Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States
title_fullStr Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States
title_full_unstemmed Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States
title_sort Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States
author Botelho, Stephanie Regina Alves
author_facet Botelho, Stephanie Regina Alves
Duarte, Macária Ferreira
Barbosa, Andreza Viveiros
Lau, Douglas
Sanches, Márcio Martinello
Fernandes, Fernanda Rausch
author_role author
author2 Duarte, Macária Ferreira
Barbosa, Andreza Viveiros
Lau, Douglas
Sanches, Márcio Martinello
Fernandes, Fernanda Rausch
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Botelho, Stephanie Regina Alves
Duarte, Macária Ferreira
Barbosa, Andreza Viveiros
Lau, Douglas
Sanches, Márcio Martinello
Fernandes, Fernanda Rausch
dc.subject.por.fl_str_mv Zea mays; exotic pest; High plains virus; plant quarantine; phytosanitary analysis
Zea mays; praga exótica; High plains virus; quarentena vegetal; análise fitossanitária
topic Zea mays; exotic pest; High plains virus; plant quarantine; phytosanitary analysis
Zea mays; praga exótica; High plains virus; quarentena vegetal; análise fitossanitária
description The objective of this work was to evaluate the phytosanitary aspect of two accessions of maize (Zea mays) seeds from the United States introduced to Brazil, regarding the presence of Wheat mosaic virus (WMoV). Two to three weeks after sowing, symptomatic leaves were tested by Elisa using specific antiserum to WMoV. The reaction was positive, and leaf samples were analyzed by real‑time PCR and amplified PCR products were sequenced. The WMoV isolates had 99 to 100% nucleotide identity with isolates from Australia and the United States. Until now, there is no report of the presence of this virus in Brazil. According to the federal law on plant protection, the plants were burned to avoid the introduction of this exotic pest in the country. The obtained results show WMoV interception in Brazil.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-03
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/20981
url https://seer.sct.embrapa.br/index.php/pab/article/view/20981
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/20981/13336
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13137
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13138
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13139
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13140
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13141
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20981/13142
dc.rights.driver.fl_str_mv Direitos autorais 2016 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2016 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária; 688-691
Pesquisa Agropecuária Brasileira; v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária; 688-691
1678-3921
0100-104x
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