Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine

Detalhes bibliográficos
Autor(a) principal: Schuck, Mariane Ruzza
Data de Publicação: 2012
Outros Autores: Biasi, Luiz Antonio, Mariano, Ada Michele, Lipski, Bernardo, Riaz, Summaira, Walker, Michael Andrew
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/11108
Resumo: The objective of this work was to assess the potential of interspecific hybridization of Vitis labruscana and Muscadinia rotundifolia by using artificial cross‑pollinations. Microsatellite markers were used to confirm interspecific hybridizations and the identity of the parental genotypes. In crosses in which M. rotundifolia was used as the female parent, no true hybrids were obtained. In the reciprocal crosses, 114 seedlings were identified as true V. labruscana x M. rotundifolia hybrids. Self‑pollination occurred in direct and in reciprocal crosses. The crossings between 'Bordo' x 'Carlos', 'Magnolia', 'Regale' and 'Roanoke', and between 'Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' and 'Roanoke' were confirmed. The 15 markers evaluated showed that two M. rotundifolia parental genotypes had the same fingerprint profile, indicating a likely planting error. The success of hybridization depends mainly on the species and on the cultivar used as the female parent. Microsatellite markers are efficient to confirm the paternity of interspecific F1 hybrids and to determine the correct identity of M. rotundifolia cultivars.
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spelling Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevineObtenção de híbridos interespecíficos e análise molecular por marcadores microssatélites em videiraMuscadinia rotundifolia; Vitis labruscana; hybrid identification; interspecific crosses; SSRsMuscadinia rotundifolia; Vitis labruscana; identificação  híbrida;  cruzamentos  interespecíficos; SSRsThe objective of this work was to assess the potential of interspecific hybridization of Vitis labruscana and Muscadinia rotundifolia by using artificial cross‑pollinations. Microsatellite markers were used to confirm interspecific hybridizations and the identity of the parental genotypes. In crosses in which M. rotundifolia was used as the female parent, no true hybrids were obtained. In the reciprocal crosses, 114 seedlings were identified as true V. labruscana x M. rotundifolia hybrids. Self‑pollination occurred in direct and in reciprocal crosses. The crossings between 'Bordo' x 'Carlos', 'Magnolia', 'Regale' and 'Roanoke', and between 'Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' and 'Roanoke' were confirmed. The 15 markers evaluated showed that two M. rotundifolia parental genotypes had the same fingerprint profile, indicating a likely planting error. The success of hybridization depends mainly on the species and on the cultivar used as the female parent. Microsatellite markers are efficient to confirm the paternity of interspecific F1 hybrids and to determine the correct identity of M. rotundifolia cultivars.O objetivo deste trabalho foi avaliar o potencial da hibridação interespecífica entre Vitis labruscana e Muscadinia rotundifolia por meio  de  polinizações  cruzadas  artificiais. Marcadores microssatélites foram utilizados para confirmar as hibridizações interespecíficas e a identidade dos parentais. Nos cruzamentos em que M. rotundifolia foi utilizada como parental feminino, nenhum híbrido verdadeiro foi obtido. Nos cruzamentos recíprocos, 114  plântulas foram identificadas como verdadeiros híbridos de V. labruscana x M. rotundifolia. Ocorreu autopolinização nos cruzamentos diretos e nos recíprocos. Foram confirmados os cruzamentos 'Bordo' x 'Carlos', 'Magnolia', 'Regale' e 'Roanoke', e 'Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' e 'Roanoke'. Os 15 marcadores avaliados revelaram que dois parentais de M. rotundifolia apresentaram o mesmo perfil genético, o que indica provável erro de plantio. O sucesso da hibridização depende principalmente da espécie e da cultivar utilizada como parental feminino. Os marcadores microssatélites são eficientes para confirmar a paternidade de híbridos interespecíficos F1 e para  determinar a correta identidade de cultivares de M. rotundifolia.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCAPES and CNPqCAPES e CNPqSchuck, Mariane RuzzaBiasi, Luiz AntonioMariano, Ada MicheleLipski, BernardoRiaz, SummairaWalker, Michael Andrew2012-02-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/11108Pesquisa Agropecuaria Brasileira; v.46, n.11, nov. 2011; 1480-1488Pesquisa Agropecuária Brasileira; v.46, n.11, nov. 2011; 1480-14881678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/11108/6696https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11108/6531info:eu-repo/semantics/openAccess2014-05-09T18:08:25Zoai:ojs.seer.sct.embrapa.br:article/11108Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-05-09T18:08:25Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine
Obtenção de híbridos interespecíficos e análise molecular por marcadores microssatélites em videira
title Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine
spellingShingle Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine
Schuck, Mariane Ruzza
Muscadinia rotundifolia; Vitis labruscana; hybrid identification; interspecific crosses; SSRs
Muscadinia rotundifolia; Vitis labruscana; identificação  híbrida;  cruzamentos  interespecíficos; SSRs
title_short Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine
title_full Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine
title_fullStr Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine
title_full_unstemmed Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine
title_sort Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine
author Schuck, Mariane Ruzza
author_facet Schuck, Mariane Ruzza
Biasi, Luiz Antonio
Mariano, Ada Michele
Lipski, Bernardo
Riaz, Summaira
Walker, Michael Andrew
author_role author
author2 Biasi, Luiz Antonio
Mariano, Ada Michele
Lipski, Bernardo
Riaz, Summaira
Walker, Michael Andrew
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv CAPES and CNPq
CAPES e CNPq
dc.contributor.author.fl_str_mv Schuck, Mariane Ruzza
Biasi, Luiz Antonio
Mariano, Ada Michele
Lipski, Bernardo
Riaz, Summaira
Walker, Michael Andrew
dc.subject.por.fl_str_mv Muscadinia rotundifolia; Vitis labruscana; hybrid identification; interspecific crosses; SSRs
Muscadinia rotundifolia; Vitis labruscana; identificação  híbrida;  cruzamentos  interespecíficos; SSRs
topic Muscadinia rotundifolia; Vitis labruscana; hybrid identification; interspecific crosses; SSRs
Muscadinia rotundifolia; Vitis labruscana; identificação  híbrida;  cruzamentos  interespecíficos; SSRs
description The objective of this work was to assess the potential of interspecific hybridization of Vitis labruscana and Muscadinia rotundifolia by using artificial cross‑pollinations. Microsatellite markers were used to confirm interspecific hybridizations and the identity of the parental genotypes. In crosses in which M. rotundifolia was used as the female parent, no true hybrids were obtained. In the reciprocal crosses, 114 seedlings were identified as true V. labruscana x M. rotundifolia hybrids. Self‑pollination occurred in direct and in reciprocal crosses. The crossings between 'Bordo' x 'Carlos', 'Magnolia', 'Regale' and 'Roanoke', and between 'Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' and 'Roanoke' were confirmed. The 15 markers evaluated showed that two M. rotundifolia parental genotypes had the same fingerprint profile, indicating a likely planting error. The success of hybridization depends mainly on the species and on the cultivar used as the female parent. Microsatellite markers are efficient to confirm the paternity of interspecific F1 hybrids and to determine the correct identity of M. rotundifolia cultivars.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-09
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/11108
url https://seer.sct.embrapa.br/index.php/pab/article/view/11108
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/11108/6696
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/11108/6531
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.46, n.11, nov. 2011; 1480-1488
Pesquisa Agropecuária Brasileira; v.46, n.11, nov. 2011; 1480-1488
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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