Aspergillus niger production of cellulases by solid‑state fermentation
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/9673 |
Resumo: | The objective of this work was to evaluate cellulase enzyme production by the filamentous fungus Aspergillus niger by solid-state fermentation of different substrates. The solid substrates sugarcane bagasse, wheat bran, soybean meal, and their mixtures were evaluated. The effects of substrate moisture contents (60, 70, and 80% in humid basis) and of the supplementation with culture media: sucrose, basic Mandels & Weber, modified Mandels & Weber, with the addition of carboxymethyl cellulose, and Czapeck Dox, were evaluated in substrate with 90% bagasse and 10% soybean meal. The highest total cellulose and endoglucanase activities, in wheat bran, were obtained after 72 hours: 0.4 and 21.0 IU g-1, respectively. There was an expressive increase in enzymatic activities as the proportion of bran in the substrate increased, in comparison to fermentation with sugarcane bagasse only. The 50% moisture content was insufficient to fully hydrate sugarcane bagasse, and the ideal humidity varies according to the medium used for supplementation and is between 70 and 80%. The modified Mandels &Weber medium shows the best result as an inductor of enzymatic activity. |
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Aspergillus niger production of cellulases by solid‑state fermentationProdução de celulases por Aspergillus niger por fermentação em estado sólidosugarcane bagasse; renewable energy; cellulosic ethanol; enzymatic hydrolysis; lignocellulosebagaço de cana-de-açúcar; energia renovável; etanol celulósico; hidrólise enzimática; lignoceluloseThe objective of this work was to evaluate cellulase enzyme production by the filamentous fungus Aspergillus niger by solid-state fermentation of different substrates. The solid substrates sugarcane bagasse, wheat bran, soybean meal, and their mixtures were evaluated. The effects of substrate moisture contents (60, 70, and 80% in humid basis) and of the supplementation with culture media: sucrose, basic Mandels & Weber, modified Mandels & Weber, with the addition of carboxymethyl cellulose, and Czapeck Dox, were evaluated in substrate with 90% bagasse and 10% soybean meal. The highest total cellulose and endoglucanase activities, in wheat bran, were obtained after 72 hours: 0.4 and 21.0 IU g-1, respectively. There was an expressive increase in enzymatic activities as the proportion of bran in the substrate increased, in comparison to fermentation with sugarcane bagasse only. The 50% moisture content was insufficient to fully hydrate sugarcane bagasse, and the ideal humidity varies according to the medium used for supplementation and is between 70 and 80%. The modified Mandels &Weber medium shows the best result as an inductor of enzymatic activity.O objetivo deste trabalho foi avaliar a produção enzimática de celulases pelo fungo filamentoso Aspergillus niger por fermentação em estado sólido de diferentes substratos. Foram avaliados os substratos sólidos bagaço de cana‑de‑açúcar, farelo de soja, farelo de trigo e misturas entre os substratos. Em substrato com 90% de bagaço e 10% de farelo de soja, avaliaram‑se os efeitos do conteúdo de umidade (60, 70 e 80%, base úmida) e da suplementação com os meios indutores da atividade enzimática: sacarose, Mandels & Weber básico, Mandels & Weber modificado, com acréscimo de carboximetilcelulose, e Czapeck Dox. As maiores atividades de celulase total e endoglucanase, em farelo de trigo, foram obtidas após 72 horas de cultivo: 0,4 e 21,0 UI g-1, respectivamente. Observou-se expressivo aumento nas atividades enzimáticas na medida em que se aumentou a proporção de farelos no substrato, em comparação à fermentação com bagaço de cana apenas. O conteúdo de umidade de 50% foi insuficiente para conseguir completa hidratação do bagaço de cana, e a umidade ideal varia de acordo com o meio utilizado para suplementação e encontra‑se entre 70 e 80%. O meio de Mandels & Weber modificado apresenta o melhor resultado como indutor da atividade enzimática.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPQ, FINEPCNPQ, FINEPRodríguez-Zúñiga, Ursula FabiolaFarinas, Cristiane SanchezBertucci Neto, VictorCouri, SoniaCrestana, Silvio2011-10-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/9673Pesquisa Agropecuaria Brasileira; v.46, n.8, ago. 2011; 912-919Pesquisa Agropecuária Brasileira; v.46, n.8, ago. 2011; 912-9191678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/9673/6500https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/4961https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/6323https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/6324https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/6325https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/6326info:eu-repo/semantics/openAccess2014-06-05T18:31:30Zoai:ojs.seer.sct.embrapa.br:article/9673Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-06-05T18:31:30Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Aspergillus niger production of cellulases by solid‑state fermentation Produção de celulases por Aspergillus niger por fermentação em estado sólido |
title |
Aspergillus niger production of cellulases by solid‑state fermentation |
spellingShingle |
Aspergillus niger production of cellulases by solid‑state fermentation Rodríguez-Zúñiga, Ursula Fabiola sugarcane bagasse; renewable energy; cellulosic ethanol; enzymatic hydrolysis; lignocellulose bagaço de cana-de-açúcar; energia renovável; etanol celulósico; hidrólise enzimática; lignocelulose |
title_short |
Aspergillus niger production of cellulases by solid‑state fermentation |
title_full |
Aspergillus niger production of cellulases by solid‑state fermentation |
title_fullStr |
Aspergillus niger production of cellulases by solid‑state fermentation |
title_full_unstemmed |
Aspergillus niger production of cellulases by solid‑state fermentation |
title_sort |
Aspergillus niger production of cellulases by solid‑state fermentation |
author |
Rodríguez-Zúñiga, Ursula Fabiola |
author_facet |
Rodríguez-Zúñiga, Ursula Fabiola Farinas, Cristiane Sanchez Bertucci Neto, Victor Couri, Sonia Crestana, Silvio |
author_role |
author |
author2 |
Farinas, Cristiane Sanchez Bertucci Neto, Victor Couri, Sonia Crestana, Silvio |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
CNPQ, FINEP CNPQ, FINEP |
dc.contributor.author.fl_str_mv |
Rodríguez-Zúñiga, Ursula Fabiola Farinas, Cristiane Sanchez Bertucci Neto, Victor Couri, Sonia Crestana, Silvio |
dc.subject.por.fl_str_mv |
sugarcane bagasse; renewable energy; cellulosic ethanol; enzymatic hydrolysis; lignocellulose bagaço de cana-de-açúcar; energia renovável; etanol celulósico; hidrólise enzimática; lignocelulose |
topic |
sugarcane bagasse; renewable energy; cellulosic ethanol; enzymatic hydrolysis; lignocellulose bagaço de cana-de-açúcar; energia renovável; etanol celulósico; hidrólise enzimática; lignocelulose |
description |
The objective of this work was to evaluate cellulase enzyme production by the filamentous fungus Aspergillus niger by solid-state fermentation of different substrates. The solid substrates sugarcane bagasse, wheat bran, soybean meal, and their mixtures were evaluated. The effects of substrate moisture contents (60, 70, and 80% in humid basis) and of the supplementation with culture media: sucrose, basic Mandels & Weber, modified Mandels & Weber, with the addition of carboxymethyl cellulose, and Czapeck Dox, were evaluated in substrate with 90% bagasse and 10% soybean meal. The highest total cellulose and endoglucanase activities, in wheat bran, were obtained after 72 hours: 0.4 and 21.0 IU g-1, respectively. There was an expressive increase in enzymatic activities as the proportion of bran in the substrate increased, in comparison to fermentation with sugarcane bagasse only. The 50% moisture content was insufficient to fully hydrate sugarcane bagasse, and the ideal humidity varies according to the medium used for supplementation and is between 70 and 80%. The modified Mandels &Weber medium shows the best result as an inductor of enzymatic activity. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-26 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/9673 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/9673 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/9673/6500 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/4961 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/6323 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/6324 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/6325 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/9673/6326 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.46, n.8, ago. 2011; 912-919 Pesquisa Agropecuária Brasileira; v.46, n.8, ago. 2011; 912-919 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416653005914112 |