Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid media

Detalhes bibliográficos
Autor(a) principal: Pereira, Jonny Everson Scherwinski
Data de Publicação: 2003
Outros Autores: Fortes, Gerson Renan de Luces
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6700
Resumo: The objective of this work was to evaluate the effects of antibiotics on the in vitro growth and multiplication rate of potato. Potato explants, cv. Baronesa, were cultivated in semi-solid and liquid multiplication culture medium. The medium was formed by MS salts, vitamins and one of the following antibiotics: ampicillin, chloramphenicol, streptomycin or tetracycline, previously selected by the bactericidal action on some culture contaminants, at 0, 32, 64, 128, 256, 512 and 1,024 mg L-1. Each treatment had three replicates of ten explants. The materials were incubated for 21 days in a growth room at 25±2°C temperature, 16-hour-light and 35 mmol m-2 s-1 radiation. The flasks with liquid culture medium were kept under continuous agitation in an orbital shaker. Only ampicillin has not affected the survival and the normal development of potato explants in the culture medium, thereby being indicated for decontamination works in vitro of this specie. The increase of the concentrations of chloramphenicol, tetracycline and streptomycin presented severe phytotoxic effects on the growth and multiplication rate of potato explants.
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spelling Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid mediaToxicidade de antibióticos no cultivo in vitro da batata em meios semi-sólido e líquidoSolanum tuberosum; phytotoxicity; micropropagation; antimicrobials; contaminationSolanum tuberosum; fitotoxicidade; micropropagação; antimicrobiano; contaminaçãoThe objective of this work was to evaluate the effects of antibiotics on the in vitro growth and multiplication rate of potato. Potato explants, cv. Baronesa, were cultivated in semi-solid and liquid multiplication culture medium. The medium was formed by MS salts, vitamins and one of the following antibiotics: ampicillin, chloramphenicol, streptomycin or tetracycline, previously selected by the bactericidal action on some culture contaminants, at 0, 32, 64, 128, 256, 512 and 1,024 mg L-1. Each treatment had three replicates of ten explants. The materials were incubated for 21 days in a growth room at 25±2°C temperature, 16-hour-light and 35 mmol m-2 s-1 radiation. The flasks with liquid culture medium were kept under continuous agitation in an orbital shaker. Only ampicillin has not affected the survival and the normal development of potato explants in the culture medium, thereby being indicated for decontamination works in vitro of this specie. The increase of the concentrations of chloramphenicol, tetracycline and streptomycin presented severe phytotoxic effects on the growth and multiplication rate of potato explants.O objetivo deste trabalho foi avaliar os efeitos fitotóxicos de antibióticos no crescimento e na taxa de multiplicação in vitro da batata. Brotações da cultivar Baronesa foram cultivadas em meio de multiplicação de consistência semi-sólida e líquida. O meio de multiplicação foi formado pelos sais e vitaminas de MS ao qual adicionou-se um dos seguintes antibióticos: ampicilina, cloranfenicol, estreptomicina e tetraciclina, previamente selecionados em razão da ação bactericida sobre contaminantes da cultura, nas concentrações de 0, 32, 64, 128, 256, 512 e 1.024 mg L-1. Por 21 dias os materiais foram mantidos em sala de crescimento a 25±2°C, 16 horas de luz e fluxo de radiação de 35 μmol m-2 s-1. Nos tratamentos em que se utilizou meio de cultura líquido, os frascos foram mantidos sob constante agitação em mesa agitadora do tipo orbital. A ampicilina foi o único antibiótico que não afetou a sobrevivência e o desenvolvimento dos explantes de batata em meio de multiplicação, podendo ser indicada para trabalhos de descontaminação in vitro dessa espécie. O aumento das concentrações de cloranfenicol, estreptomicina e tetraciclina no meio de cultura apresentou efeitos fitotóxicos severos sobre o crescimento e taxa de multiplicação do material vegetal.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraPereira, Jonny Everson ScherwinskiFortes, Gerson Renan de Luces2003-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6700Pesquisa Agropecuaria Brasileira; v.38, n.11, nov. 2003; 1273-1279Pesquisa Agropecuária Brasileira; v.38, n.11, nov. 2003; 1273-12791678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6700/3757info:eu-repo/semantics/openAccess2014-08-11T19:42:02Zoai:ojs.seer.sct.embrapa.br:article/6700Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-11T19:42:02Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid media
Toxicidade de antibióticos no cultivo in vitro da batata em meios semi-sólido e líquido
title Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid media
spellingShingle Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid media
Pereira, Jonny Everson Scherwinski
Solanum tuberosum; phytotoxicity; micropropagation; antimicrobials; contamination
Solanum tuberosum; fitotoxicidade; micropropagação; antimicrobiano; contaminação
title_short Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid media
title_full Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid media
title_fullStr Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid media
title_full_unstemmed Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid media
title_sort Antibiotics toxicity on the in vitro potato cultivation in semi-solid and liquid media
author Pereira, Jonny Everson Scherwinski
author_facet Pereira, Jonny Everson Scherwinski
Fortes, Gerson Renan de Luces
author_role author
author2 Fortes, Gerson Renan de Luces
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Pereira, Jonny Everson Scherwinski
Fortes, Gerson Renan de Luces
dc.subject.por.fl_str_mv Solanum tuberosum; phytotoxicity; micropropagation; antimicrobials; contamination
Solanum tuberosum; fitotoxicidade; micropropagação; antimicrobiano; contaminação
topic Solanum tuberosum; phytotoxicity; micropropagation; antimicrobials; contamination
Solanum tuberosum; fitotoxicidade; micropropagação; antimicrobiano; contaminação
description The objective of this work was to evaluate the effects of antibiotics on the in vitro growth and multiplication rate of potato. Potato explants, cv. Baronesa, were cultivated in semi-solid and liquid multiplication culture medium. The medium was formed by MS salts, vitamins and one of the following antibiotics: ampicillin, chloramphenicol, streptomycin or tetracycline, previously selected by the bactericidal action on some culture contaminants, at 0, 32, 64, 128, 256, 512 and 1,024 mg L-1. Each treatment had three replicates of ten explants. The materials were incubated for 21 days in a growth room at 25±2°C temperature, 16-hour-light and 35 mmol m-2 s-1 radiation. The flasks with liquid culture medium were kept under continuous agitation in an orbital shaker. Only ampicillin has not affected the survival and the normal development of potato explants in the culture medium, thereby being indicated for decontamination works in vitro of this specie. The increase of the concentrations of chloramphenicol, tetracycline and streptomycin presented severe phytotoxic effects on the growth and multiplication rate of potato explants.
publishDate 2003
dc.date.none.fl_str_mv 2003-11-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6700
url https://seer.sct.embrapa.br/index.php/pab/article/view/6700
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6700/3757
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.38, n.11, nov. 2003; 1273-1279
Pesquisa Agropecuária Brasileira; v.38, n.11, nov. 2003; 1273-1279
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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