Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometry

Detalhes bibliográficos
Autor(a) principal: Campos, José Marcello Salabert de
Data de Publicação: 2009
Outros Autores: Calderano, Clara de Azevedo, Pereira, Antônio Vander, Davide, Lisete Chamma, Viccini, Lyderson Facio, Santos, Marcelo de Oliveira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/1271
Resumo: The objectives of this study were to establish an efficient protocol for somatic embryogenesis in triploid hybrids between napiergrass (Pennisetum purpureum Schumach.) and pearl millet (P. glaucum (L.) R. Br.), and to assess the genomic stability by flow cytometry of the plants obtained in vitro. Somatic embryogenesis and plant regeneration were successfully established from mature zygotic embryos of napiergrass and pearl millet hybrids. Four treatments with 2,4-dichlorophenoxyacetic acid (2,4-D) at 0, 1, 2 e 3 mg L-1 were tested for embryogenic calli induction and two treatments with inositol at 1 e 2 g L-1 were tested for plant regeneration. The treatments were arranged in a completely randomized design. The optimum hormone combinations were 2 mg L-1 of 2,4-D for embryogenic callus induction, and 1 g L-1 of inositol for embryos conversion and plant regeneration. The analysis of DNA content by flow cytometry of the regenerated plantlets indicated that no ploidy changes had been induced during somatic embryogenesis and plant regeneration. The nuclear DNA content and ploidy levels of the regenerated plants were stable and homogeneous in comparison to those of the control plants. There was no occurrence of karyological instability in the regeneration system utilized for Pennisetum hybrid.
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spelling Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometryEmbriogênese somática em híbridos de Pennisetum sp. e avaliação de estabilidade genômica por citometriaPennisetum glaucum; Pennisetum purpureum; tissue culturePennisetum glaucum; Pennisetum purpureum; cultura de tecidosThe objectives of this study were to establish an efficient protocol for somatic embryogenesis in triploid hybrids between napiergrass (Pennisetum purpureum Schumach.) and pearl millet (P. glaucum (L.) R. Br.), and to assess the genomic stability by flow cytometry of the plants obtained in vitro. Somatic embryogenesis and plant regeneration were successfully established from mature zygotic embryos of napiergrass and pearl millet hybrids. Four treatments with 2,4-dichlorophenoxyacetic acid (2,4-D) at 0, 1, 2 e 3 mg L-1 were tested for embryogenic calli induction and two treatments with inositol at 1 e 2 g L-1 were tested for plant regeneration. The treatments were arranged in a completely randomized design. The optimum hormone combinations were 2 mg L-1 of 2,4-D for embryogenic callus induction, and 1 g L-1 of inositol for embryos conversion and plant regeneration. The analysis of DNA content by flow cytometry of the regenerated plantlets indicated that no ploidy changes had been induced during somatic embryogenesis and plant regeneration. The nuclear DNA content and ploidy levels of the regenerated plants were stable and homogeneous in comparison to those of the control plants. There was no occurrence of karyological instability in the regeneration system utilized for Pennisetum hybrid.Os objetivos deste trabalho foram estabelecer um protocolo eficiente de embriogênese somática, em híbridos triploides entre capim-elefante (Pennisetum purpureum Schumach.) e milheto (P. glaucum (L.) R. Br.), e avaliar por citometria de fluxo a estabilidade genômica das plantas obtidas in vitro. A embriogênese somática e a regeneração das plantas foram estabelecidas a partir de embriões zigóticos maduros de híbridos entre capim-elefante e milheto. Foram testados quatro tratamentos com 2,4-ácido diclorofenoxiacético (2,4-D), nas concentrações 0, 1, 2 e 3 mg L-1, para indução de calos embriogênicos, e dois tratamentos com inositol a 1 e 2 g L-1, para regeneração das plantas. Os tratamentos foram dispostos em delineamento inteiramente ao acaso. A combinação ótima de hormônios foi de 2 mg L-1 de 2,4-D, para indução de calos embriogênicos, e de 1 g L-1 de inositol, para conversão de embriões e regeneração de plantas. A análise de quantidade de DNA, por citometria de fluxo das plantas regeneradas, indicou a não ocorrência de alterações em ploidia durante a embriogênese somática e a regeneração das plantas. A quantidade de DNA nuclear e a ploidia das plantas regeneradas foram estáveis e homogêneas em comparação às das plantas controle. Não ocorreu instabilidade cariotípica no sistema de regeneração usado para híbridos de Pennisetum.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Campos, José Marcello Salabert deCalderano, Clara de AzevedoPereira, Antônio VanderDavide, Lisete ChammaViccini, Lyderson FacioSantos, Marcelo de Oliveira2009-03-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/1271Pesquisa Agropecuaria Brasileira; v.44, n.1, jan. 2009; 38-44Pesquisa Agropecuária Brasileira; v.44, n.1, jan. 2009; 38-441678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/1271/5626https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1271/887https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1271/888https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1271/889info:eu-repo/semantics/openAccess2014-11-18T17:27:51Zoai:ojs.seer.sct.embrapa.br:article/1271Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-11-18T17:27:51Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometry
Embriogênese somática em híbridos de Pennisetum sp. e avaliação de estabilidade genômica por citometria
title Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometry
spellingShingle Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometry
Campos, José Marcello Salabert de
Pennisetum glaucum; Pennisetum purpureum; tissue culture
Pennisetum glaucum; Pennisetum purpureum; cultura de tecidos
title_short Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometry
title_full Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometry
title_fullStr Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometry
title_full_unstemmed Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometry
title_sort Somatic embryogenesis in hybrids of Pennisetum sp. and genomic stability evaluation by cytometry
author Campos, José Marcello Salabert de
author_facet Campos, José Marcello Salabert de
Calderano, Clara de Azevedo
Pereira, Antônio Vander
Davide, Lisete Chamma
Viccini, Lyderson Facio
Santos, Marcelo de Oliveira
author_role author
author2 Calderano, Clara de Azevedo
Pereira, Antônio Vander
Davide, Lisete Chamma
Viccini, Lyderson Facio
Santos, Marcelo de Oliveira
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.contributor.author.fl_str_mv Campos, José Marcello Salabert de
Calderano, Clara de Azevedo
Pereira, Antônio Vander
Davide, Lisete Chamma
Viccini, Lyderson Facio
Santos, Marcelo de Oliveira
dc.subject.por.fl_str_mv Pennisetum glaucum; Pennisetum purpureum; tissue culture
Pennisetum glaucum; Pennisetum purpureum; cultura de tecidos
topic Pennisetum glaucum; Pennisetum purpureum; tissue culture
Pennisetum glaucum; Pennisetum purpureum; cultura de tecidos
description The objectives of this study were to establish an efficient protocol for somatic embryogenesis in triploid hybrids between napiergrass (Pennisetum purpureum Schumach.) and pearl millet (P. glaucum (L.) R. Br.), and to assess the genomic stability by flow cytometry of the plants obtained in vitro. Somatic embryogenesis and plant regeneration were successfully established from mature zygotic embryos of napiergrass and pearl millet hybrids. Four treatments with 2,4-dichlorophenoxyacetic acid (2,4-D) at 0, 1, 2 e 3 mg L-1 were tested for embryogenic calli induction and two treatments with inositol at 1 e 2 g L-1 were tested for plant regeneration. The treatments were arranged in a completely randomized design. The optimum hormone combinations were 2 mg L-1 of 2,4-D for embryogenic callus induction, and 1 g L-1 of inositol for embryos conversion and plant regeneration. The analysis of DNA content by flow cytometry of the regenerated plantlets indicated that no ploidy changes had been induced during somatic embryogenesis and plant regeneration. The nuclear DNA content and ploidy levels of the regenerated plants were stable and homogeneous in comparison to those of the control plants. There was no occurrence of karyological instability in the regeneration system utilized for Pennisetum hybrid.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-04
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/1271
url https://seer.sct.embrapa.br/index.php/pab/article/view/1271
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/1271/5626
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1271/887
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1271/888
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1271/889
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.44, n.1, jan. 2009; 38-44
Pesquisa Agropecuária Brasileira; v.44, n.1, jan. 2009; 38-44
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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