Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasm

Detalhes bibliográficos
Autor(a) principal: Silva, Rafael de Carvalho
Data de Publicação: 2011
Outros Autores: Camillo, Julceia, Luis, Zanderluce Gomes, Scherwinski-Pereira, Jonny Everson
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/9954
Resumo: The objective of this work was to evaluate the germinative potential and the morphoanatomical features of leaves in seedlings of physic nut from cryopreserved germplasm. Initially, seeds were evaluated regarding desiccation every 24 hours for up to 120 hours. Then, seeds were desiccated for 0, 24, and 48 hours, and evaluated monthly as to germinative potential, before and after storage in liquid nitrogen by direct immersion and fast freezing. To characterize the viability of seeds that did not germinate after the evaluation period, the zygotic embryos were immersed in tetrazolium solution. Morphoanatomical features of leaves were determined after cryopreservation by histological evaluation. Physic nut seeds tolerated desiccation to critical levels and cryopreservation with moisture content around 8%. Seed desiccation to lower values, associated with longer periods of exposition to liquid nitrogen, causes abnormalities in seedlings, damages leaf cells and tissues, and affects negatively germination.
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spelling Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasmPotencial germinativo e morfoanatomia foliar de plântulas de pinhão‑manso originadas de germoplasma criopreservadoJatropha curcas; ex situ conservation; cryopreservation; desiccation; seed physiology; toleranceJatropha curcas; conservação ex situ; criopreservação; dessecação; fisiologia de sementes; tolerânciaThe objective of this work was to evaluate the germinative potential and the morphoanatomical features of leaves in seedlings of physic nut from cryopreserved germplasm. Initially, seeds were evaluated regarding desiccation every 24 hours for up to 120 hours. Then, seeds were desiccated for 0, 24, and 48 hours, and evaluated monthly as to germinative potential, before and after storage in liquid nitrogen by direct immersion and fast freezing. To characterize the viability of seeds that did not germinate after the evaluation period, the zygotic embryos were immersed in tetrazolium solution. Morphoanatomical features of leaves were determined after cryopreservation by histological evaluation. Physic nut seeds tolerated desiccation to critical levels and cryopreservation with moisture content around 8%. Seed desiccation to lower values, associated with longer periods of exposition to liquid nitrogen, causes abnormalities in seedlings, damages leaf cells and tissues, and affects negatively germination.O objetivo deste trabalho foi avaliar o potencial germinativo e caracterizar morfoanatomicamente a estrutura foliar de plântulas de pinhão-manso originadas de germoplasma criopreservado. Inicialmente, as sementes foram avaliadas quanto à dessecação, a cada 24 horas, por até 120 horas. Posteriormente, as sementes foram dessecadas por 0, 24 e 48 horas e avaliadas mensalmente quanto ao potencial germinativo, antes e após armazenamento em nitrogênio líquido por imersão direta e congelamento rápido. Para caracterizar a viabilidade das sementes que não germinaram após o período de avaliação, os embriões zigóticos foram imersos em solução de tetrazólio. A morfoanatomia foliar após criopreservação foi caracterizada por meio de estudos histológicos. As sementes de pinhão-manso toleraram a dessecação a níveis críticos e a criopreservação com umidade em torno de 8%. A dessecação das sementes até valores baixos, associada a períodos prolongados de exposição ao nitrogênio líquido, causa anormalidade de plântulas, danifica as células e os tecidos das folhas, e afeta negativamente a germinação. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPqFinepSilva, Rafael de CarvalhoCamillo, JulceiaLuis, Zanderluce GomesScherwinski-Pereira, Jonny Everson2011-10-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/9954Pesquisa Agropecuaria Brasileira; v.46, n.8, ago. 2011; 836-844Pesquisa Agropecuária Brasileira; v.46, n.8, ago. 2011; 836-8441678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/9954/6507info:eu-repo/semantics/openAccess2014-06-05T16:21:59Zoai:ojs.seer.sct.embrapa.br:article/9954Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-06-05T16:21:59Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasm
Potencial germinativo e morfoanatomia foliar de plântulas de pinhão‑manso originadas de germoplasma criopreservado
title Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasm
spellingShingle Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasm
Silva, Rafael de Carvalho
Jatropha curcas; ex situ conservation; cryopreservation; desiccation; seed physiology; tolerance
Jatropha curcas; conservação ex situ; criopreservação; dessecação; fisiologia de sementes; tolerância
title_short Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasm
title_full Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasm
title_fullStr Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasm
title_full_unstemmed Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasm
title_sort Germinative potential and leaf morphoanatomy of physic nut seedlings from cryopreserved germplasm
author Silva, Rafael de Carvalho
author_facet Silva, Rafael de Carvalho
Camillo, Julceia
Luis, Zanderluce Gomes
Scherwinski-Pereira, Jonny Everson
author_role author
author2 Camillo, Julceia
Luis, Zanderluce Gomes
Scherwinski-Pereira, Jonny Everson
author2_role author
author
author
dc.contributor.none.fl_str_mv
CNPq
Finep
dc.contributor.author.fl_str_mv Silva, Rafael de Carvalho
Camillo, Julceia
Luis, Zanderluce Gomes
Scherwinski-Pereira, Jonny Everson
dc.subject.por.fl_str_mv Jatropha curcas; ex situ conservation; cryopreservation; desiccation; seed physiology; tolerance
Jatropha curcas; conservação ex situ; criopreservação; dessecação; fisiologia de sementes; tolerância
topic Jatropha curcas; ex situ conservation; cryopreservation; desiccation; seed physiology; tolerance
Jatropha curcas; conservação ex situ; criopreservação; dessecação; fisiologia de sementes; tolerância
description The objective of this work was to evaluate the germinative potential and the morphoanatomical features of leaves in seedlings of physic nut from cryopreserved germplasm. Initially, seeds were evaluated regarding desiccation every 24 hours for up to 120 hours. Then, seeds were desiccated for 0, 24, and 48 hours, and evaluated monthly as to germinative potential, before and after storage in liquid nitrogen by direct immersion and fast freezing. To characterize the viability of seeds that did not germinate after the evaluation period, the zygotic embryos were immersed in tetrazolium solution. Morphoanatomical features of leaves were determined after cryopreservation by histological evaluation. Physic nut seeds tolerated desiccation to critical levels and cryopreservation with moisture content around 8%. Seed desiccation to lower values, associated with longer periods of exposition to liquid nitrogen, causes abnormalities in seedlings, damages leaf cells and tissues, and affects negatively germination.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-27
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/9954
url https://seer.sct.embrapa.br/index.php/pab/article/view/9954
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/9954/6507
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.46, n.8, ago. 2011; 836-844
Pesquisa Agropecuária Brasileira; v.46, n.8, ago. 2011; 836-844
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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