Enzymatic activity and bacterial community profile in soil under solarization e biofumigation
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/131 |
Resumo: | The objective of this work was to evaluate the effects of solarization and biofumigation on the soil microbial communities, by means of beta-glucosidase activity and 16S rDNA PCR-DGGE analyses. Solarization with a plastic covering of the soil took place over two, four and six months, and the soils were biofumigated by the addition of 2 and 5% (v/v) of chicken litter to the soil. Right after the plastic cover removal and after 30 days, beta-glucosidase was lower than in the nonsolarizated control. After 60 days, there were no longer significant differences in beta-glucosidase activity between treatments. The addition of 5% chicken litter stimulated beta-glucosidase activity. Bacterial community profile was influenced by solarization time, regardless of time of plastic cover removal. There was no effect of chicken litter amendments over the bacterial community structure. Solarization affects beta-glucosidase activity but, after 60 days, its effects are no longer detectable, differently of the observed data regarding soil bacterial community structure by PCR-DGGE. Biofumigation stimulates beta-glicosidase activity, but it doesn't affect the bacterial community structure. |
id |
EMBRAPA-4_41fe2f7d14c53d21932185865f185809 |
---|---|
oai_identifier_str |
oai:ojs.seer.sct.embrapa.br:article/131 |
network_acronym_str |
EMBRAPA-4 |
network_name_str |
Pesquisa Agropecuária Brasileira (Online) |
repository_id_str |
|
spelling |
Enzymatic activity and bacterial community profile in soil under solarization e biofumigationAtividade enzimática e perfil da comunidade bacteriana em solo submetido à solarização e biofumigaçãobeta-glucosidase; chicken litter; microbial diversity; microbial ecology; soil management; 16S rDNAbeta-glicosidase; cama-de-frango; diversidade microbiana; ecologia microbiana; manejo do solo; 16S rDNAThe objective of this work was to evaluate the effects of solarization and biofumigation on the soil microbial communities, by means of beta-glucosidase activity and 16S rDNA PCR-DGGE analyses. Solarization with a plastic covering of the soil took place over two, four and six months, and the soils were biofumigated by the addition of 2 and 5% (v/v) of chicken litter to the soil. Right after the plastic cover removal and after 30 days, beta-glucosidase was lower than in the nonsolarizated control. After 60 days, there were no longer significant differences in beta-glucosidase activity between treatments. The addition of 5% chicken litter stimulated beta-glucosidase activity. Bacterial community profile was influenced by solarization time, regardless of time of plastic cover removal. There was no effect of chicken litter amendments over the bacterial community structure. Solarization affects beta-glucosidase activity but, after 60 days, its effects are no longer detectable, differently of the observed data regarding soil bacterial community structure by PCR-DGGE. Biofumigation stimulates beta-glicosidase activity, but it doesn't affect the bacterial community structure.O objetivo deste trabalho foi avaliar o efeito da solarização e da biofumigação sobre a comunidade microbiana do solo, por meio da atividade da enzima beta-glicosidase e do perfil do 16S rDNA, determinado com PCR-DGGE. A solarização do solo, com cobertura de plástico, foi feita por períodos de dois, quatro e seis meses, e a biofumigação foi realizada pela incorporação de 2 e 5% (v/v) de cama-de-frango ao solo. Logo após a retirada da cobertura de plástico e aos 30 dias após a remoção, a atividade da beta-glicosidase foi menor em relação ao tratamento não solarizado. Aos 60 dias, não foram mais observadas diferenças entre os tratamentos. A adição de cama-de-frango a 5% estimulou a atividade da beta-glicosidase. O perfil da estrutura da comunidade bacteriana foi influenciado pelo tempo de solarização, independentemente da época da retirada da cobertura de plástico. Não foi observado efeito da adição de cama-de-frango ao solo, no perfil da comunidade. A solarização afeta a atividade da beta-glicosidase, mas esses efeitos não são mais detectáveis após 60 dias da retirada da cobertura de plástico, diferentemente do que foi observado em relação à estrutura da comunidade bacteriana por PCR-DGGE. A biofumigação estimula a atividade da beta-glicosidase, mas não afeta o perfil da comunidade microbiana.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPq, CAPESPassos, Samuel RibeiroJunior, Fábio Bueno dos ReisRumjanek, Norma GouvêaMendes, Ieda de CarvalhoBaptista, Miriam JosefinaXavier, Gustavo Ribeiro2008-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/131Pesquisa Agropecuaria Brasileira; v.43, n.7, jul. 2008; 879-885Pesquisa Agropecuária Brasileira; v.43, n.7, jul. 2008; 879-8851678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/131/5468https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/131/107info:eu-repo/semantics/openAccess2012-06-17T11:41:59Zoai:ojs.seer.sct.embrapa.br:article/131Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:41:59Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Enzymatic activity and bacterial community profile in soil under solarization e biofumigation Atividade enzimática e perfil da comunidade bacteriana em solo submetido à solarização e biofumigação |
title |
Enzymatic activity and bacterial community profile in soil under solarization e biofumigation |
spellingShingle |
Enzymatic activity and bacterial community profile in soil under solarization e biofumigation Passos, Samuel Ribeiro beta-glucosidase; chicken litter; microbial diversity; microbial ecology; soil management; 16S rDNA beta-glicosidase; cama-de-frango; diversidade microbiana; ecologia microbiana; manejo do solo; 16S rDNA |
title_short |
Enzymatic activity and bacterial community profile in soil under solarization e biofumigation |
title_full |
Enzymatic activity and bacterial community profile in soil under solarization e biofumigation |
title_fullStr |
Enzymatic activity and bacterial community profile in soil under solarization e biofumigation |
title_full_unstemmed |
Enzymatic activity and bacterial community profile in soil under solarization e biofumigation |
title_sort |
Enzymatic activity and bacterial community profile in soil under solarization e biofumigation |
author |
Passos, Samuel Ribeiro |
author_facet |
Passos, Samuel Ribeiro Junior, Fábio Bueno dos Reis Rumjanek, Norma Gouvêa Mendes, Ieda de Carvalho Baptista, Miriam Josefina Xavier, Gustavo Ribeiro |
author_role |
author |
author2 |
Junior, Fábio Bueno dos Reis Rumjanek, Norma Gouvêa Mendes, Ieda de Carvalho Baptista, Miriam Josefina Xavier, Gustavo Ribeiro |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
CNPq, CAPES |
dc.contributor.author.fl_str_mv |
Passos, Samuel Ribeiro Junior, Fábio Bueno dos Reis Rumjanek, Norma Gouvêa Mendes, Ieda de Carvalho Baptista, Miriam Josefina Xavier, Gustavo Ribeiro |
dc.subject.por.fl_str_mv |
beta-glucosidase; chicken litter; microbial diversity; microbial ecology; soil management; 16S rDNA beta-glicosidase; cama-de-frango; diversidade microbiana; ecologia microbiana; manejo do solo; 16S rDNA |
topic |
beta-glucosidase; chicken litter; microbial diversity; microbial ecology; soil management; 16S rDNA beta-glicosidase; cama-de-frango; diversidade microbiana; ecologia microbiana; manejo do solo; 16S rDNA |
description |
The objective of this work was to evaluate the effects of solarization and biofumigation on the soil microbial communities, by means of beta-glucosidase activity and 16S rDNA PCR-DGGE analyses. Solarization with a plastic covering of the soil took place over two, four and six months, and the soils were biofumigated by the addition of 2 and 5% (v/v) of chicken litter to the soil. Right after the plastic cover removal and after 30 days, beta-glucosidase was lower than in the nonsolarizated control. After 60 days, there were no longer significant differences in beta-glucosidase activity between treatments. The addition of 5% chicken litter stimulated beta-glucosidase activity. Bacterial community profile was influenced by solarization time, regardless of time of plastic cover removal. There was no effect of chicken litter amendments over the bacterial community structure. Solarization affects beta-glucosidase activity but, after 60 days, its effects are no longer detectable, differently of the observed data regarding soil bacterial community structure by PCR-DGGE. Biofumigation stimulates beta-glicosidase activity, but it doesn't affect the bacterial community structure. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-28 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/131 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/131 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/131/5468 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/131/107 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.43, n.7, jul. 2008; 879-885 Pesquisa Agropecuária Brasileira; v.43, n.7, jul. 2008; 879-885 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
_version_ |
1793416657564073984 |