Enzymatic activity and bacterial community profile in soil under solarization e biofumigation

Detalhes bibliográficos
Autor(a) principal: Passos, Samuel Ribeiro
Data de Publicação: 2008
Outros Autores: Junior, Fábio Bueno dos Reis, Rumjanek, Norma Gouvêa, Mendes, Ieda de Carvalho, Baptista, Miriam Josefina, Xavier, Gustavo Ribeiro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/131
Resumo: The objective of this work was to evaluate the effects of solarization and biofumigation on the soil microbial communities, by means of beta-glucosidase activity and 16S rDNA PCR-DGGE analyses. Solarization with a plastic covering of the soil took place over two, four and six months, and the soils were biofumigated by the addition of 2 and 5% (v/v) of chicken litter to the soil. Right after the plastic cover removal and after 30 days, beta-glucosidase was lower than in the nonsolarizated control. After 60 days, there were no longer significant differences in beta-glucosidase activity between treatments. The addition of 5% chicken litter stimulated beta-glucosidase activity. Bacterial community profile was influenced by solarization time, regardless of time of plastic cover removal. There was no effect of chicken litter amendments over the bacterial community structure. Solarization affects beta-glucosidase activity but, after 60 days, its effects are no longer detectable, differently of the observed data regarding soil bacterial community structure by PCR-DGGE. Biofumigation stimulates beta-glicosidase activity, but it doesn't affect the bacterial community structure.
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spelling Enzymatic activity and bacterial community profile in soil under solarization e biofumigationAtividade enzimática e perfil da comunidade bacteriana em solo submetido à solarização e biofumigaçãobeta-glucosidase; chicken litter; microbial diversity; microbial ecology; soil management; 16S rDNAbeta-glicosidase; cama-de-frango; diversidade microbiana; ecologia microbiana; manejo do solo; 16S rDNAThe objective of this work was to evaluate the effects of solarization and biofumigation on the soil microbial communities, by means of beta-glucosidase activity and 16S rDNA PCR-DGGE analyses. Solarization with a plastic covering of the soil took place over two, four and six months, and the soils were biofumigated by the addition of 2 and 5% (v/v) of chicken litter to the soil. Right after the plastic cover removal and after 30 days, beta-glucosidase was lower than in the nonsolarizated control. After 60 days, there were no longer significant differences in beta-glucosidase activity between treatments. The addition of 5% chicken litter stimulated beta-glucosidase activity. Bacterial community profile was influenced by solarization time, regardless of time of plastic cover removal. There was no effect of chicken litter amendments over the bacterial community structure. Solarization affects beta-glucosidase activity but, after 60 days, its effects are no longer detectable, differently of the observed data regarding soil bacterial community structure by PCR-DGGE. Biofumigation stimulates beta-glicosidase activity, but it doesn't affect the bacterial community structure.O objetivo deste trabalho foi avaliar o efeito da solarização e da biofumigação sobre a comunidade microbiana do solo, por meio da atividade da enzima beta-glicosidase e do perfil do 16S rDNA, determinado com PCR-DGGE. A solarização do solo, com cobertura de plástico, foi feita por períodos de dois, quatro e seis meses, e a biofumigação foi realizada pela incorporação de 2 e 5% (v/v) de cama-de-frango ao solo. Logo após a retirada da cobertura de plástico e aos 30 dias após a remoção, a atividade da beta-glicosidase foi menor em relação ao tratamento não solarizado. Aos 60 dias, não foram mais observadas diferenças entre os tratamentos. A adição de cama-de-frango a 5% estimulou a atividade da beta-glicosidase. O perfil da estrutura da comunidade bacteriana foi influenciado pelo tempo de solarização, independentemente da época da retirada da cobertura de plástico. Não foi observado efeito da adição de cama-de-frango ao solo, no perfil da comunidade. A solarização afeta a atividade da beta-glicosidase, mas esses efeitos não são mais detectáveis após 60 dias da retirada da cobertura de plástico, diferentemente do que foi observado em relação à estrutura da comunidade bacteriana por PCR-DGGE. A biofumigação estimula a atividade da beta-glicosidase, mas não afeta o perfil da comunidade microbiana.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPq, CAPESPassos, Samuel RibeiroJunior, Fábio Bueno dos ReisRumjanek, Norma GouvêaMendes, Ieda de CarvalhoBaptista, Miriam JosefinaXavier, Gustavo Ribeiro2008-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/131Pesquisa Agropecuaria Brasileira; v.43, n.7, jul. 2008; 879-885Pesquisa Agropecuária Brasileira; v.43, n.7, jul. 2008; 879-8851678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/131/5468https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/131/107info:eu-repo/semantics/openAccess2012-06-17T11:41:59Zoai:ojs.seer.sct.embrapa.br:article/131Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:41:59Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Enzymatic activity and bacterial community profile in soil under solarization e biofumigation
Atividade enzimática e perfil da comunidade bacteriana em solo submetido à solarização e biofumigação
title Enzymatic activity and bacterial community profile in soil under solarization e biofumigation
spellingShingle Enzymatic activity and bacterial community profile in soil under solarization e biofumigation
Passos, Samuel Ribeiro
beta-glucosidase; chicken litter; microbial diversity; microbial ecology; soil management; 16S rDNA
beta-glicosidase; cama-de-frango; diversidade microbiana; ecologia microbiana; manejo do solo; 16S rDNA
title_short Enzymatic activity and bacterial community profile in soil under solarization e biofumigation
title_full Enzymatic activity and bacterial community profile in soil under solarization e biofumigation
title_fullStr Enzymatic activity and bacterial community profile in soil under solarization e biofumigation
title_full_unstemmed Enzymatic activity and bacterial community profile in soil under solarization e biofumigation
title_sort Enzymatic activity and bacterial community profile in soil under solarization e biofumigation
author Passos, Samuel Ribeiro
author_facet Passos, Samuel Ribeiro
Junior, Fábio Bueno dos Reis
Rumjanek, Norma Gouvêa
Mendes, Ieda de Carvalho
Baptista, Miriam Josefina
Xavier, Gustavo Ribeiro
author_role author
author2 Junior, Fábio Bueno dos Reis
Rumjanek, Norma Gouvêa
Mendes, Ieda de Carvalho
Baptista, Miriam Josefina
Xavier, Gustavo Ribeiro
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
CNPq, CAPES
dc.contributor.author.fl_str_mv Passos, Samuel Ribeiro
Junior, Fábio Bueno dos Reis
Rumjanek, Norma Gouvêa
Mendes, Ieda de Carvalho
Baptista, Miriam Josefina
Xavier, Gustavo Ribeiro
dc.subject.por.fl_str_mv beta-glucosidase; chicken litter; microbial diversity; microbial ecology; soil management; 16S rDNA
beta-glicosidase; cama-de-frango; diversidade microbiana; ecologia microbiana; manejo do solo; 16S rDNA
topic beta-glucosidase; chicken litter; microbial diversity; microbial ecology; soil management; 16S rDNA
beta-glicosidase; cama-de-frango; diversidade microbiana; ecologia microbiana; manejo do solo; 16S rDNA
description The objective of this work was to evaluate the effects of solarization and biofumigation on the soil microbial communities, by means of beta-glucosidase activity and 16S rDNA PCR-DGGE analyses. Solarization with a plastic covering of the soil took place over two, four and six months, and the soils were biofumigated by the addition of 2 and 5% (v/v) of chicken litter to the soil. Right after the plastic cover removal and after 30 days, beta-glucosidase was lower than in the nonsolarizated control. After 60 days, there were no longer significant differences in beta-glucosidase activity between treatments. The addition of 5% chicken litter stimulated beta-glucosidase activity. Bacterial community profile was influenced by solarization time, regardless of time of plastic cover removal. There was no effect of chicken litter amendments over the bacterial community structure. Solarization affects beta-glucosidase activity but, after 60 days, its effects are no longer detectable, differently of the observed data regarding soil bacterial community structure by PCR-DGGE. Biofumigation stimulates beta-glicosidase activity, but it doesn't affect the bacterial community structure.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-28
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/131
url https://seer.sct.embrapa.br/index.php/pab/article/view/131
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/131/5468
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/131/107
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.43, n.7, jul. 2008; 879-885
Pesquisa Agropecuária Brasileira; v.43, n.7, jul. 2008; 879-885
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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