Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryos

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Valtemir Gonçalves
Data de Publicação: 2000
Outros Autores: Sanábio, Deny, Souza, Cleber Nascimento de, Lopes, Paulo Sergio Nascimento, Bocardo, Marcelo Ramazotti, Pasqual, Moacir
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/5682
Resumo: This work utilized fruits of a single plant obtained from the cross between Citrus limonia Osb. c Poncirus trifoliata L. Raf. Seeds were removed and after excision, all the embryos, regardless their development stages, were inoculated in MS medium added with GA3 (0.0, 0.01, 0.1 and 1.0 mg/L) and activated coal (0.0, 0.5, 1.0 and 2.0 g/L) in every possible combination. These treatments remained for 48 hours in the dark and afterwards in growth room at the temperature of 27 ± 1oC with 16 hours lighting. After 40 days, seedlings were evaluated by the lengths of the aerial part and root system and percent of survival. The concentration of 0.1 mg/L of GA3 interacts negatively in culture medium containing up to 2.0 g/L of activated coal. The concentration of 0.01 mg/L of GA3 associated to activated coal concentration of 0.5 g/L to 2.0 g/L maximized the percentage of embryo survival. Embryos showed the highest length of root (4.5 cm) and shoot (2.1 cm) when cultivated in activated coal at 0.5 g/L and 2.0 g/L, respectively.
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spelling Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryosEfeitos de ácido giberélico e carvão ativado no cultivo in vitro de Citrus limonia Osbeck x Poncirus trifoliata (L.) Rafembryo culture; breeding of citrinecultura de embriões; melhoramento genético de citrosThis work utilized fruits of a single plant obtained from the cross between Citrus limonia Osb. c Poncirus trifoliata L. Raf. Seeds were removed and after excision, all the embryos, regardless their development stages, were inoculated in MS medium added with GA3 (0.0, 0.01, 0.1 and 1.0 mg/L) and activated coal (0.0, 0.5, 1.0 and 2.0 g/L) in every possible combination. These treatments remained for 48 hours in the dark and afterwards in growth room at the temperature of 27 ± 1oC with 16 hours lighting. After 40 days, seedlings were evaluated by the lengths of the aerial part and root system and percent of survival. The concentration of 0.1 mg/L of GA3 interacts negatively in culture medium containing up to 2.0 g/L of activated coal. The concentration of 0.01 mg/L of GA3 associated to activated coal concentration of 0.5 g/L to 2.0 g/L maximized the percentage of embryo survival. Embryos showed the highest length of root (4.5 cm) and shoot (2.1 cm) when cultivated in activated coal at 0.5 g/L and 2.0 g/L, respectively.Pesquisou-se o desenvolvimento in vitro de embriões de um híbrido do cruzamento Citrus limonia Osb. c Poncirus trifoliata (L.) Raf. em meio MS, acrescido de GA3 (0,0, 0,01, 0,1 e 1,0 mg/L) e carvão ativado (0,0, 0,5, 1,0 e 2,0 g/L), em todas as combinações possíveis. Após a excisão das sementes os embriões obtidos, independentemente dos estádios, foram inoculados nos respectivos meios de cultura. Cada tratamento permaneceu por 48 horas no escuro e 40 dias em sala de crescimento à temperatura de 27 ± 1oC, com 16 horas de luminosidade. Após esse período, as plântulas foram avaliadas, considerando-se o porcentual de sobrevivência e comprimento da haste caulinar e do sistema radicular. Verificou-se que a concentação de 0,1 mg/L de GA3 interagiu antagonicamente em meio de cultivo contendo até 2,0 g/L de carvão ativado. O GA3 a 0,01 mg/L associado a concentrações de carvão ativado a partir de 0,5 g/L até 2,0 g/L, maximizou o porcentual de sobrevivência dos embriões. Os embriões apresentaram o maior comprimento de raiz (4,5 cm) e da haste caulinar (2,1 cm) com carvão ativado na concentração de 0,5 g/L e 2,0 g/L, respectivamente.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraRibeiro, Valtemir GonçalvesSanábio, DenySouza, Cleber Nascimento deLopes, Paulo Sergio NascimentoBocardo, Marcelo RamazottiPasqual, Moacir2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/5682Pesquisa Agropecuaria Brasileira; v.35, n.1, jan. 2000; 27-30Pesquisa Agropecuária Brasileira; v.35, n.1, jan. 2000; 27-301678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/5682/2786info:eu-repo/semantics/openAccess2010-07-29T17:16:01Zoai:ojs.seer.sct.embrapa.br:article/5682Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2010-07-29T17:16:01Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryos
Efeitos de ácido giberélico e carvão ativado no cultivo in vitro de Citrus limonia Osbeck x Poncirus trifoliata (L.) Raf
title Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryos
spellingShingle Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryos
Ribeiro, Valtemir Gonçalves
embryo culture; breeding of citrine
cultura de embriões; melhoramento genético de citros
title_short Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryos
title_full Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryos
title_fullStr Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryos
title_full_unstemmed Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryos
title_sort Effects of gibberellic acid (GA3) and activated coal on in vitro culture of Citrus limonia Osbeck x Poncirus trifoliata L. Raf. embryos
author Ribeiro, Valtemir Gonçalves
author_facet Ribeiro, Valtemir Gonçalves
Sanábio, Deny
Souza, Cleber Nascimento de
Lopes, Paulo Sergio Nascimento
Bocardo, Marcelo Ramazotti
Pasqual, Moacir
author_role author
author2 Sanábio, Deny
Souza, Cleber Nascimento de
Lopes, Paulo Sergio Nascimento
Bocardo, Marcelo Ramazotti
Pasqual, Moacir
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Ribeiro, Valtemir Gonçalves
Sanábio, Deny
Souza, Cleber Nascimento de
Lopes, Paulo Sergio Nascimento
Bocardo, Marcelo Ramazotti
Pasqual, Moacir
dc.subject.por.fl_str_mv embryo culture; breeding of citrine
cultura de embriões; melhoramento genético de citros
topic embryo culture; breeding of citrine
cultura de embriões; melhoramento genético de citros
description This work utilized fruits of a single plant obtained from the cross between Citrus limonia Osb. c Poncirus trifoliata L. Raf. Seeds were removed and after excision, all the embryos, regardless their development stages, were inoculated in MS medium added with GA3 (0.0, 0.01, 0.1 and 1.0 mg/L) and activated coal (0.0, 0.5, 1.0 and 2.0 g/L) in every possible combination. These treatments remained for 48 hours in the dark and afterwards in growth room at the temperature of 27 ± 1oC with 16 hours lighting. After 40 days, seedlings were evaluated by the lengths of the aerial part and root system and percent of survival. The concentration of 0.1 mg/L of GA3 interacts negatively in culture medium containing up to 2.0 g/L of activated coal. The concentration of 0.01 mg/L of GA3 associated to activated coal concentration of 0.5 g/L to 2.0 g/L maximized the percentage of embryo survival. Embryos showed the highest length of root (4.5 cm) and shoot (2.1 cm) when cultivated in activated coal at 0.5 g/L and 2.0 g/L, respectively.
publishDate 2000
dc.date.none.fl_str_mv 2000-01-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5682
url https://seer.sct.embrapa.br/index.php/pab/article/view/5682
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5682/2786
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.35, n.1, jan. 2000; 27-30
Pesquisa Agropecuária Brasileira; v.35, n.1, jan. 2000; 27-30
1678-3921
0100-104x
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repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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