Sulfonamides quantification in milk by high performace liquid chromatography via azoderivatives

Detalhes bibliográficos
Autor(a) principal: Marques, Marcelo Volpatto
Data de Publicação: 2009
Outros Autores: Naciuk, Fabrício Fredo, Mello, Ana Maria de Souza, Seibel, Nair Maria, Santos, João Gabriel Rosa dos, Fontoura, Luiz Antonio Mazzini
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/1214
Resumo: The objective of this work was to evaluate an analytical method for the simultaneous measurement of sulfathiazole, sulfamethazine, and sulfadimethoxine in milk, in the form of azoderivatives, through the high performance liquid chromatography (HPLC). The isolation and concentration of sulfas were carried out by solid phase extraction (C18) with methanol as eluent. After extraction, analytes were transformed in diazonium salts and coupled with resorcinol. Derivatives were quantified by HPLC (C8 column, l = 430 nm, MeOH/KH2PO4 aq). Recoveries were obtained between 65 and 78%.
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spelling Sulfonamides quantification in milk by high performace liquid chromatography via azoderivativesQuantificação de sulfonamidas em leite por cromatografia líquida de alta eficiência via azoderivadosHPLC; solid phase extraction; resorcinol; diazonium saltsCLAE; extração em fase sólida; resorcinol; sais de diazônioThe objective of this work was to evaluate an analytical method for the simultaneous measurement of sulfathiazole, sulfamethazine, and sulfadimethoxine in milk, in the form of azoderivatives, through the high performance liquid chromatography (HPLC). The isolation and concentration of sulfas were carried out by solid phase extraction (C18) with methanol as eluent. After extraction, analytes were transformed in diazonium salts and coupled with resorcinol. Derivatives were quantified by HPLC (C8 column, l = 430 nm, MeOH/KH2PO4 aq). Recoveries were obtained between 65 and 78%.O objetivo deste trabalho foi avaliar uma alternativa para a quantificação simultânea de sulfatiazol, sulfametazina e sulfadimetoxina no leite, na forma de azoderivados, por meio de cromatografia líquida de alta eficiência (CLAE). O isolamento e concentração das sulfas foram conduzidos por extração em fase sólida (C18), com metanol como eluente. Após a extração, os analitos foram transformados em sais de diazônio e submetidos ao acoplamento com resorcinol. Os derivados foram separados e quantificados por CLAE (coluna C8, l = 430 nm, MeOH/KH2PO4 aq.). Foram obtidas recuperações entre 65 e 78%.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraPAMVet-ANVISAMarques, Marcelo VolpattoNaciuk, Fabrício FredoMello, Ana Maria de SouzaSeibel, Nair MariaSantos, João Gabriel Rosa dosFontoura, Luiz Antonio Mazzini2009-01-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/1214Pesquisa Agropecuaria Brasileira; v.43, n.12, dez. 2008; 1817-1820Pesquisa Agropecuária Brasileira; v.43, n.12, dez. 2008; 1817-18201678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/1214/5597https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1214/837info:eu-repo/semantics/openAccess2012-06-17T11:45:39Zoai:ojs.seer.sct.embrapa.br:article/1214Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:45:39Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Sulfonamides quantification in milk by high performace liquid chromatography via azoderivatives
Quantificação de sulfonamidas em leite por cromatografia líquida de alta eficiência via azoderivados
title Sulfonamides quantification in milk by high performace liquid chromatography via azoderivatives
spellingShingle Sulfonamides quantification in milk by high performace liquid chromatography via azoderivatives
Marques, Marcelo Volpatto
HPLC; solid phase extraction; resorcinol; diazonium salts
CLAE; extração em fase sólida; resorcinol; sais de diazônio
title_short Sulfonamides quantification in milk by high performace liquid chromatography via azoderivatives
title_full Sulfonamides quantification in milk by high performace liquid chromatography via azoderivatives
title_fullStr Sulfonamides quantification in milk by high performace liquid chromatography via azoderivatives
title_full_unstemmed Sulfonamides quantification in milk by high performace liquid chromatography via azoderivatives
title_sort Sulfonamides quantification in milk by high performace liquid chromatography via azoderivatives
author Marques, Marcelo Volpatto
author_facet Marques, Marcelo Volpatto
Naciuk, Fabrício Fredo
Mello, Ana Maria de Souza
Seibel, Nair Maria
Santos, João Gabriel Rosa dos
Fontoura, Luiz Antonio Mazzini
author_role author
author2 Naciuk, Fabrício Fredo
Mello, Ana Maria de Souza
Seibel, Nair Maria
Santos, João Gabriel Rosa dos
Fontoura, Luiz Antonio Mazzini
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
PAMVet-ANVISA
dc.contributor.author.fl_str_mv Marques, Marcelo Volpatto
Naciuk, Fabrício Fredo
Mello, Ana Maria de Souza
Seibel, Nair Maria
Santos, João Gabriel Rosa dos
Fontoura, Luiz Antonio Mazzini
dc.subject.por.fl_str_mv HPLC; solid phase extraction; resorcinol; diazonium salts
CLAE; extração em fase sólida; resorcinol; sais de diazônio
topic HPLC; solid phase extraction; resorcinol; diazonium salts
CLAE; extração em fase sólida; resorcinol; sais de diazônio
description The objective of this work was to evaluate an analytical method for the simultaneous measurement of sulfathiazole, sulfamethazine, and sulfadimethoxine in milk, in the form of azoderivatives, through the high performance liquid chromatography (HPLC). The isolation and concentration of sulfas were carried out by solid phase extraction (C18) with methanol as eluent. After extraction, analytes were transformed in diazonium salts and coupled with resorcinol. Derivatives were quantified by HPLC (C8 column, l = 430 nm, MeOH/KH2PO4 aq). Recoveries were obtained between 65 and 78%.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-06
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/1214
url https://seer.sct.embrapa.br/index.php/pab/article/view/1214
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/1214/5597
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/1214/837
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.43, n.12, dez. 2008; 1817-1820
Pesquisa Agropecuária Brasileira; v.43, n.12, dez. 2008; 1817-1820
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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