Genetic characterization of the Moxotó goat breed using RAPD markers

Detalhes bibliográficos
Autor(a) principal: Oliveira, Rejane Rodrigues de
Data de Publicação: 2005
Outros Autores: Egito, Andréa Alves do, Ribeiro, Maria Norma, Paiva, Samuel Rezende, Albuquerque, Maria do Socorro Maués, Castro, Silvia Ribeiro, Mariante, Arthur da Silvia, Adrião, Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6949
Resumo: The objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.
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spelling Genetic characterization of the Moxotó goat breed using RAPD markersCaracterização genética da raça caprina Moxotó por marcadores RAPDCapra hircus; native breeds; population structure; conservation geneticsCapra hircus; raças nativas; estrutura de populações; conservação genéticaThe objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.O objetivo deste estudo foi verificar a diversidade genética entre e dentro de sete populações de cabras Moxotó (n = 264) dos Estados de Pernambuco, Paraíba e Rio Grande do Norte, usando a técnica de RAPD (Poliformismo de DNA Amplificados ao Acaso). A raça Moxotó, assim como outras naturalizadas, sofre grande erosão genética devido à miscigenação indiscriminada com outras raças existentes na Região Nordeste. A caracterização genética desta raça é essencial para programas de conservação e melhoramento. Os marcadores moleculares são uma ferramenta útil na estimativa da diversidade genética de diferentes populações. O DNA foi extraído a partir de linfócitos utilizando-se um protocolo não orgânico. Os 16 primers utilizados foram selecionados a partir de 120 oligonucleotídeos e geraram 56 bandas polimórficas. A análise de variância molecular (AMOVA) demonstrou que a maior parte da variabilidade genética total (71,55%) ocorreu em virtude das diferenças entre indivíduos dentro das populações, enquanto 21,21% ocorriam entre as populações. A análise da variância entre os pares de populações demonstra que as populações de Floresta, PE x Angicos, RN apresentam o menor valor de diferenciação interpopulacional (8,9%), indicando menor variabilidade entre elas. A distância genética de Nei variou entre 0,0546 e 0,1868 nas populações. O dendrograma gerado demonstra que a raça Canindé, utilizada como outgroup, agrupa-se com as populações de Moxotó, indicando a possível origem comum das raças naturalizadas. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraOliveira, Rejane Rodrigues deEgito, Andréa Alves doRibeiro, Maria NormaPaiva, Samuel RezendeAlbuquerque, Maria do Socorro MauésCastro, Silvia RibeiroMariante, Arthur da SilviaAdrião, Manuel2005-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6949Pesquisa Agropecuaria Brasileira; v.40, n.3, mar. 2005; 233-239Pesquisa Agropecuária Brasileira; v.40, n.3, mar. 2005; 233-2391678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/6949/3997info:eu-repo/semantics/openAccess2014-09-22T19:01:22Zoai:ojs.seer.sct.embrapa.br:article/6949Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-22T19:01:22Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic characterization of the Moxotó goat breed using RAPD markers
Caracterização genética da raça caprina Moxotó por marcadores RAPD
title Genetic characterization of the Moxotó goat breed using RAPD markers
spellingShingle Genetic characterization of the Moxotó goat breed using RAPD markers
Oliveira, Rejane Rodrigues de
Capra hircus; native breeds; population structure; conservation genetics
Capra hircus; raças nativas; estrutura de populações; conservação genética
title_short Genetic characterization of the Moxotó goat breed using RAPD markers
title_full Genetic characterization of the Moxotó goat breed using RAPD markers
title_fullStr Genetic characterization of the Moxotó goat breed using RAPD markers
title_full_unstemmed Genetic characterization of the Moxotó goat breed using RAPD markers
title_sort Genetic characterization of the Moxotó goat breed using RAPD markers
author Oliveira, Rejane Rodrigues de
author_facet Oliveira, Rejane Rodrigues de
Egito, Andréa Alves do
Ribeiro, Maria Norma
Paiva, Samuel Rezende
Albuquerque, Maria do Socorro Maués
Castro, Silvia Ribeiro
Mariante, Arthur da Silvia
Adrião, Manuel
author_role author
author2 Egito, Andréa Alves do
Ribeiro, Maria Norma
Paiva, Samuel Rezende
Albuquerque, Maria do Socorro Maués
Castro, Silvia Ribeiro
Mariante, Arthur da Silvia
Adrião, Manuel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Oliveira, Rejane Rodrigues de
Egito, Andréa Alves do
Ribeiro, Maria Norma
Paiva, Samuel Rezende
Albuquerque, Maria do Socorro Maués
Castro, Silvia Ribeiro
Mariante, Arthur da Silvia
Adrião, Manuel
dc.subject.por.fl_str_mv Capra hircus; native breeds; population structure; conservation genetics
Capra hircus; raças nativas; estrutura de populações; conservação genética
topic Capra hircus; native breeds; population structure; conservation genetics
Capra hircus; raças nativas; estrutura de populações; conservação genética
description The objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.
publishDate 2005
dc.date.none.fl_str_mv 2005-03-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6949
url https://seer.sct.embrapa.br/index.php/pab/article/view/6949
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6949/3997
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.40, n.3, mar. 2005; 233-239
Pesquisa Agropecuária Brasileira; v.40, n.3, mar. 2005; 233-239
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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