Effects of synthetic auxins on the in vitro rooting of apple tree

• Autor(a) principal: Centellas, Alberto Quezada
• Data de Publicação: 1999
• Outros Autores: Fortes, Gerson Renan de Luces, Muller, Nilvane Terezinha Ghellar, Zanol, Geni Varmen, Flores, Rejane, Gottinari, Rosete Aparecida
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Pesquisa Agropecuária Brasileira (Online)
• Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/5162
• topic: micropropagation; growth substances; indol acetic acid; indol butiric acid; naphtalene acetic acid; Malus domestica; micropropagation; growth substances; indol acetic acid; indol butiric acid; naphtalene acetic acid; Malus domestica

Apple shoots (Malus domestica, Borkh), cv. Fred Hough derived from in vitro multiplication process were inoculated in MS and MS/2 basal media added by growth substances indol acetic acid (IAA); indol butiric acid (IBA) and naphtalene acetic acid (NAA) at 0, 1, 3 and 5 µM. The media also included: MS vitamins, myo-inositol (100.0 mg/L); sucrose (30.0 g/L); agar (6.0 g/L). The pH was adjusted to 5.8 before autoclaving. The treatments were incubated in a growth room at 25±2oC, 16 hours photoperiod under light intensity of 2,000 lux during 30 days. The treatments were replicated five times. Each replicate was composed by a 250 mL flask containing 40 mL medium with five explants. The medium MS/2 in all the tested concentrations was better than MS. The NAA and IBA, both at 3.0 µM on MS/2 showed similar effects as percentage of rooting and number of roots are concerned. However, NAA treated explants presented a higher callus incidence at the base of shoots along with thicker roots. IAA had a better response when used in higher concentrations but it did not achieve the performance of IBA ou NAA.