Growth regulators on potato meristem culture

Detalhes bibliográficos
Autor(a) principal: Lopes, José Flavio
Data de Publicação: 2014
Outros Autores: Peters, José Antonio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/15531
Resumo: Fourteen culture media were tested for differentiation and growth of potato (Solanum tuberosum L. cv. Baronesa) meristems with one to three primordial leaflets (2-4 mm long). Seven of these media were variations from Murashige & Skoog (MS) basic medium with the addition of 100 mg/l of myo-inositol, 30 g/l of sucrose and 10 g/l of agar (MS). The other seven media were variations from White basic medium with the addition of 20 g/l of sucrose and 10 g/l of agar (WB). Both MS and WB were supplemented with the various concentrations of growth regulators, such as two auxins – alphanaphthaleneacetic acid (ANA) and 2,4-dichlorofenoxiacetic acid (2,4-D) - and two cytokinins - 6-furfurilaminopurine (KIN) and 6.benzilaminopurine (BAP). The culture media were adjusted to pH 5.6, after autoclaved at 105ºC for 10 min. The meristems were explanted onto flasks with 25 ml of the medium, which were then kept in a growth chamber with a temperature of 22 ± 2ºC. Continuous light with an intensity of 600 lux provided by cool, white fluorescent tubes was utilized. The first signs of differentiation occurred about 30 days after placement of the meristem on the media. Small outgrowths were evident on the surface of a developed callus tissue or directly on the explants. These outgrowths elongated and rooted abundantly when transferred to MS. The seedlings were transplanted to 200 - ml pots containing a sterilizer mixture of sand and soil (1:1 ratio). MS were best than WB to root outgrowths, plant vigor and development. The best treatment was MS-6 (0,01 mg/l of 2,4-D and 0,04 mg/l of BAP) followed by MS-5 (0.02 mg/l of ANA and 0,04 mg/l of BAP). For MS-5 rooting was delayed.
id EMBRAPA-4_5e3f320fda722725f795f9084c5a4940
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/15531
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling Growth regulators on potato meristem cultureReguladores de crescimento na cultura de meristemas de batatapotato; Solanum tuberosum; apical buds; tissue culturebatata (Solanum tuberosum L.); gemas apicais; cultura de tecidoFourteen culture media were tested for differentiation and growth of potato (Solanum tuberosum L. cv. Baronesa) meristems with one to three primordial leaflets (2-4 mm long). Seven of these media were variations from Murashige & Skoog (MS) basic medium with the addition of 100 mg/l of myo-inositol, 30 g/l of sucrose and 10 g/l of agar (MS). The other seven media were variations from White basic medium with the addition of 20 g/l of sucrose and 10 g/l of agar (WB). Both MS and WB were supplemented with the various concentrations of growth regulators, such as two auxins – alphanaphthaleneacetic acid (ANA) and 2,4-dichlorofenoxiacetic acid (2,4-D) - and two cytokinins - 6-furfurilaminopurine (KIN) and 6.benzilaminopurine (BAP). The culture media were adjusted to pH 5.6, after autoclaved at 105ºC for 10 min. The meristems were explanted onto flasks with 25 ml of the medium, which were then kept in a growth chamber with a temperature of 22 ± 2ºC. Continuous light with an intensity of 600 lux provided by cool, white fluorescent tubes was utilized. The first signs of differentiation occurred about 30 days after placement of the meristem on the media. Small outgrowths were evident on the surface of a developed callus tissue or directly on the explants. These outgrowths elongated and rooted abundantly when transferred to MS. The seedlings were transplanted to 200 - ml pots containing a sterilizer mixture of sand and soil (1:1 ratio). MS were best than WB to root outgrowths, plant vigor and development. The best treatment was MS-6 (0,01 mg/l of 2,4-D and 0,04 mg/l of BAP) followed by MS-5 (0.02 mg/l of ANA and 0,04 mg/l of BAP). For MS-5 rooting was delayed.Foram testados quatorze tratamentos na diferenciação de meristemas apicais de batata com um a três primórdios foliares. Sete deles foram obtidos a partir do meio básico de Murashige & Skoog com vitaminas acrescido de 100 mg/l de mio-inositol, 30 g/l de sacarose e 10 g/l de ágar (MS). Os outros sete, a partir do meio básico e vitaminas de White acrescido de 20 g/l de sacarose e 10 g/l de ágar (WB). Acrescentaram-se tanto em MS quanto em WB diferentes concentrações de reguladores de crescimento tais como duas auxinas - ácido naftalenoacético (ANA), ácido 2,4-diclofenoxiacético (2,4-D) - e duas citoquininas - cinetina (CIN) e 6-benzilaminopurina (BAP). Os meios de cultura, depois de autoclavados a 105ºC durante dez minutos, tiveram o pH ajustado a 5,6. Após a inoculação, foram mantidos em sala de crescimento sob luz fluorescente contínua (600 lux), à temperatura de 22 ± 2ºC. Os primeiros sinais de diferenciação ocorreram em torno de 30 dias após a colocação dos meristemas nos meios de cultura. Surgiram gemas da superfície de uma massa calosa ou diretamente dos explantes. As gemas desenvolveram-se em sistemas caulinares que enraizaram abundantemente quando transferidos para MS. As plântulas obtidas foram transplantadas para recipientes de 200 ml contendo terra ou vermiculita e areia esterilizada na proporção 1:1, obtendo-se bom pegamento e normal desenvolvimento posterior. MS apresentou-se superior a WB nos aspectos de desenvolvimento, enraizamento e vigor de plântulas. O melhor tratamento foi o MS-6 (0,1 ppm de 2,4-D e 0,04 ppm de BAP), seguido do MS-5 (0,02 ppm de ANA e 0,04 ppm de BAP). Nesse último, o enraizamento foi mais tardio.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraLopes, José FlavioPeters, José Antonio2014-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/15531Pesquisa Agropecuaria Brasileira; v.17, n.1, jan. 1982; 57-60Pesquisa Agropecuária Brasileira; v.17, n.1, jan. 1982; 57-601678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/15531/9500info:eu-repo/semantics/openAccess2014-04-15T19:45:17Zoai:ojs.seer.sct.embrapa.br:article/15531Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-15T19:45:17Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Growth regulators on potato meristem culture
Reguladores de crescimento na cultura de meristemas de batata
title Growth regulators on potato meristem culture
spellingShingle Growth regulators on potato meristem culture
Lopes, José Flavio
potato; Solanum tuberosum; apical buds; tissue culture
batata (Solanum tuberosum L.); gemas apicais; cultura de tecido
title_short Growth regulators on potato meristem culture
title_full Growth regulators on potato meristem culture
title_fullStr Growth regulators on potato meristem culture
title_full_unstemmed Growth regulators on potato meristem culture
title_sort Growth regulators on potato meristem culture
author Lopes, José Flavio
author_facet Lopes, José Flavio
Peters, José Antonio
author_role author
author2 Peters, José Antonio
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Lopes, José Flavio
Peters, José Antonio
dc.subject.por.fl_str_mv potato; Solanum tuberosum; apical buds; tissue culture
batata (Solanum tuberosum L.); gemas apicais; cultura de tecido
topic potato; Solanum tuberosum; apical buds; tissue culture
batata (Solanum tuberosum L.); gemas apicais; cultura de tecido
description Fourteen culture media were tested for differentiation and growth of potato (Solanum tuberosum L. cv. Baronesa) meristems with one to three primordial leaflets (2-4 mm long). Seven of these media were variations from Murashige & Skoog (MS) basic medium with the addition of 100 mg/l of myo-inositol, 30 g/l of sucrose and 10 g/l of agar (MS). The other seven media were variations from White basic medium with the addition of 20 g/l of sucrose and 10 g/l of agar (WB). Both MS and WB were supplemented with the various concentrations of growth regulators, such as two auxins – alphanaphthaleneacetic acid (ANA) and 2,4-dichlorofenoxiacetic acid (2,4-D) - and two cytokinins - 6-furfurilaminopurine (KIN) and 6.benzilaminopurine (BAP). The culture media were adjusted to pH 5.6, after autoclaved at 105ºC for 10 min. The meristems were explanted onto flasks with 25 ml of the medium, which were then kept in a growth chamber with a temperature of 22 ± 2ºC. Continuous light with an intensity of 600 lux provided by cool, white fluorescent tubes was utilized. The first signs of differentiation occurred about 30 days after placement of the meristem on the media. Small outgrowths were evident on the surface of a developed callus tissue or directly on the explants. These outgrowths elongated and rooted abundantly when transferred to MS. The seedlings were transplanted to 200 - ml pots containing a sterilizer mixture of sand and soil (1:1 ratio). MS were best than WB to root outgrowths, plant vigor and development. The best treatment was MS-6 (0,01 mg/l of 2,4-D and 0,04 mg/l of BAP) followed by MS-5 (0.02 mg/l of ANA and 0,04 mg/l of BAP). For MS-5 rooting was delayed.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-15
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/15531
url https://seer.sct.embrapa.br/index.php/pab/article/view/15531
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/15531/9500
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.17, n.1, jan. 1982; 57-60
Pesquisa Agropecuária Brasileira; v.17, n.1, jan. 1982; 57-60
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416655912566784