Growth regulators on potato meristem culture
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/15531 |
Resumo: | Fourteen culture media were tested for differentiation and growth of potato (Solanum tuberosum L. cv. Baronesa) meristems with one to three primordial leaflets (2-4 mm long). Seven of these media were variations from Murashige & Skoog (MS) basic medium with the addition of 100 mg/l of myo-inositol, 30 g/l of sucrose and 10 g/l of agar (MS). The other seven media were variations from White basic medium with the addition of 20 g/l of sucrose and 10 g/l of agar (WB). Both MS and WB were supplemented with the various concentrations of growth regulators, such as two auxins – alphanaphthaleneacetic acid (ANA) and 2,4-dichlorofenoxiacetic acid (2,4-D) - and two cytokinins - 6-furfurilaminopurine (KIN) and 6.benzilaminopurine (BAP). The culture media were adjusted to pH 5.6, after autoclaved at 105ºC for 10 min. The meristems were explanted onto flasks with 25 ml of the medium, which were then kept in a growth chamber with a temperature of 22 ± 2ºC. Continuous light with an intensity of 600 lux provided by cool, white fluorescent tubes was utilized. The first signs of differentiation occurred about 30 days after placement of the meristem on the media. Small outgrowths were evident on the surface of a developed callus tissue or directly on the explants. These outgrowths elongated and rooted abundantly when transferred to MS. The seedlings were transplanted to 200 - ml pots containing a sterilizer mixture of sand and soil (1:1 ratio). MS were best than WB to root outgrowths, plant vigor and development. The best treatment was MS-6 (0,01 mg/l of 2,4-D and 0,04 mg/l of BAP) followed by MS-5 (0.02 mg/l of ANA and 0,04 mg/l of BAP). For MS-5 rooting was delayed. |
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Growth regulators on potato meristem cultureReguladores de crescimento na cultura de meristemas de batatapotato; Solanum tuberosum; apical buds; tissue culturebatata (Solanum tuberosum L.); gemas apicais; cultura de tecidoFourteen culture media were tested for differentiation and growth of potato (Solanum tuberosum L. cv. Baronesa) meristems with one to three primordial leaflets (2-4 mm long). Seven of these media were variations from Murashige & Skoog (MS) basic medium with the addition of 100 mg/l of myo-inositol, 30 g/l of sucrose and 10 g/l of agar (MS). The other seven media were variations from White basic medium with the addition of 20 g/l of sucrose and 10 g/l of agar (WB). Both MS and WB were supplemented with the various concentrations of growth regulators, such as two auxins – alphanaphthaleneacetic acid (ANA) and 2,4-dichlorofenoxiacetic acid (2,4-D) - and two cytokinins - 6-furfurilaminopurine (KIN) and 6.benzilaminopurine (BAP). The culture media were adjusted to pH 5.6, after autoclaved at 105ºC for 10 min. The meristems were explanted onto flasks with 25 ml of the medium, which were then kept in a growth chamber with a temperature of 22 ± 2ºC. Continuous light with an intensity of 600 lux provided by cool, white fluorescent tubes was utilized. The first signs of differentiation occurred about 30 days after placement of the meristem on the media. Small outgrowths were evident on the surface of a developed callus tissue or directly on the explants. These outgrowths elongated and rooted abundantly when transferred to MS. The seedlings were transplanted to 200 - ml pots containing a sterilizer mixture of sand and soil (1:1 ratio). MS were best than WB to root outgrowths, plant vigor and development. The best treatment was MS-6 (0,01 mg/l of 2,4-D and 0,04 mg/l of BAP) followed by MS-5 (0.02 mg/l of ANA and 0,04 mg/l of BAP). For MS-5 rooting was delayed.Foram testados quatorze tratamentos na diferenciação de meristemas apicais de batata com um a três primórdios foliares. Sete deles foram obtidos a partir do meio básico de Murashige & Skoog com vitaminas acrescido de 100 mg/l de mio-inositol, 30 g/l de sacarose e 10 g/l de ágar (MS). Os outros sete, a partir do meio básico e vitaminas de White acrescido de 20 g/l de sacarose e 10 g/l de ágar (WB). Acrescentaram-se tanto em MS quanto em WB diferentes concentrações de reguladores de crescimento tais como duas auxinas - ácido naftalenoacético (ANA), ácido 2,4-diclofenoxiacético (2,4-D) - e duas citoquininas - cinetina (CIN) e 6-benzilaminopurina (BAP). Os meios de cultura, depois de autoclavados a 105ºC durante dez minutos, tiveram o pH ajustado a 5,6. Após a inoculação, foram mantidos em sala de crescimento sob luz fluorescente contínua (600 lux), à temperatura de 22 ± 2ºC. Os primeiros sinais de diferenciação ocorreram em torno de 30 dias após a colocação dos meristemas nos meios de cultura. Surgiram gemas da superfície de uma massa calosa ou diretamente dos explantes. As gemas desenvolveram-se em sistemas caulinares que enraizaram abundantemente quando transferidos para MS. As plântulas obtidas foram transplantadas para recipientes de 200 ml contendo terra ou vermiculita e areia esterilizada na proporção 1:1, obtendo-se bom pegamento e normal desenvolvimento posterior. MS apresentou-se superior a WB nos aspectos de desenvolvimento, enraizamento e vigor de plântulas. O melhor tratamento foi o MS-6 (0,1 ppm de 2,4-D e 0,04 ppm de BAP), seguido do MS-5 (0,02 ppm de ANA e 0,04 ppm de BAP). Nesse último, o enraizamento foi mais tardio.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraLopes, José FlavioPeters, José Antonio2014-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/15531Pesquisa Agropecuaria Brasileira; v.17, n.1, jan. 1982; 57-60Pesquisa Agropecuária Brasileira; v.17, n.1, jan. 1982; 57-601678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/15531/9500info:eu-repo/semantics/openAccess2014-04-15T19:45:17Zoai:ojs.seer.sct.embrapa.br:article/15531Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-15T19:45:17Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Growth regulators on potato meristem culture Reguladores de crescimento na cultura de meristemas de batata |
title |
Growth regulators on potato meristem culture |
spellingShingle |
Growth regulators on potato meristem culture Lopes, José Flavio potato; Solanum tuberosum; apical buds; tissue culture batata (Solanum tuberosum L.); gemas apicais; cultura de tecido |
title_short |
Growth regulators on potato meristem culture |
title_full |
Growth regulators on potato meristem culture |
title_fullStr |
Growth regulators on potato meristem culture |
title_full_unstemmed |
Growth regulators on potato meristem culture |
title_sort |
Growth regulators on potato meristem culture |
author |
Lopes, José Flavio |
author_facet |
Lopes, José Flavio Peters, José Antonio |
author_role |
author |
author2 |
Peters, José Antonio |
author2_role |
author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Lopes, José Flavio Peters, José Antonio |
dc.subject.por.fl_str_mv |
potato; Solanum tuberosum; apical buds; tissue culture batata (Solanum tuberosum L.); gemas apicais; cultura de tecido |
topic |
potato; Solanum tuberosum; apical buds; tissue culture batata (Solanum tuberosum L.); gemas apicais; cultura de tecido |
description |
Fourteen culture media were tested for differentiation and growth of potato (Solanum tuberosum L. cv. Baronesa) meristems with one to three primordial leaflets (2-4 mm long). Seven of these media were variations from Murashige & Skoog (MS) basic medium with the addition of 100 mg/l of myo-inositol, 30 g/l of sucrose and 10 g/l of agar (MS). The other seven media were variations from White basic medium with the addition of 20 g/l of sucrose and 10 g/l of agar (WB). Both MS and WB were supplemented with the various concentrations of growth regulators, such as two auxins – alphanaphthaleneacetic acid (ANA) and 2,4-dichlorofenoxiacetic acid (2,4-D) - and two cytokinins - 6-furfurilaminopurine (KIN) and 6.benzilaminopurine (BAP). The culture media were adjusted to pH 5.6, after autoclaved at 105ºC for 10 min. The meristems were explanted onto flasks with 25 ml of the medium, which were then kept in a growth chamber with a temperature of 22 ± 2ºC. Continuous light with an intensity of 600 lux provided by cool, white fluorescent tubes was utilized. The first signs of differentiation occurred about 30 days after placement of the meristem on the media. Small outgrowths were evident on the surface of a developed callus tissue or directly on the explants. These outgrowths elongated and rooted abundantly when transferred to MS. The seedlings were transplanted to 200 - ml pots containing a sterilizer mixture of sand and soil (1:1 ratio). MS were best than WB to root outgrowths, plant vigor and development. The best treatment was MS-6 (0,01 mg/l of 2,4-D and 0,04 mg/l of BAP) followed by MS-5 (0.02 mg/l of ANA and 0,04 mg/l of BAP). For MS-5 rooting was delayed. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-15 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/15531 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/15531 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/15531/9500 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.17, n.1, jan. 1982; 57-60 Pesquisa Agropecuária Brasileira; v.17, n.1, jan. 1982; 57-60 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
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Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416655912566784 |