Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary test

Detalhes bibliográficos
Autor(a) principal: Kessler, Raul Henrique
Data de Publicação: 2014
Outros Autores: Sacco, Ana Maria S., Jesus, Elane F. de, Madruga, Cláudio Roberto
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/14698
Resumo: With the objective of developing local strains of Babesia bovis and Babesia bigemina attenuated according to the Australian method, sixteen rapid passages of B. bovis in splenectomized calves and four slow passages of B. bigemina in intact calves were made. A preliminary test of the virulence and antigenicity of these strains in two-year old Hereford steers in the field showed that: 1) the B. bovis strain was of low virulence as the inoculated animals did not present clinical signs of the disease during the vaccine reaction; 2) the B. bigemina strain was still too virulent since three of five inoculated steers presented visible clinical signs, although all recovered spontaneously from the infection; 3) both strains were highly antigenic as all inoculated animals were positive for the indirect fluorescent antibody test and resisted the homologous challenge with the respective virulent strain, while all of the non-vaccinated controls became acutely ill and although properly medicated, one died.
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spelling Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary testDesenvolvimento de cepas vivas atenuadas de Babesia bovis e Babesia bigemina: teste preliminarBabesiosis; vaccine; attenuation; bovine passagebabesiose; vacina; atenuação; passagem bovinaWith the objective of developing local strains of Babesia bovis and Babesia bigemina attenuated according to the Australian method, sixteen rapid passages of B. bovis in splenectomized calves and four slow passages of B. bigemina in intact calves were made. A preliminary test of the virulence and antigenicity of these strains in two-year old Hereford steers in the field showed that: 1) the B. bovis strain was of low virulence as the inoculated animals did not present clinical signs of the disease during the vaccine reaction; 2) the B. bigemina strain was still too virulent since three of five inoculated steers presented visible clinical signs, although all recovered spontaneously from the infection; 3) both strains were highly antigenic as all inoculated animals were positive for the indirect fluorescent antibody test and resisted the homologous challenge with the respective virulent strain, while all of the non-vaccinated controls became acutely ill and although properly medicated, one died.Foram efetivadas 16 passagens de B. bovis em bezerros esplenectomizados e quatro passagens de B. bigemina em bezerros intactos com o objetivo de desenvolver cepas atenuadas para posterior uso como vacina, de acordo com a tecnologia australiana. O teste preliminar da virulência e antigenicidade destas cepas, realizado a campo, em novilhos Hereford de dois anos de idade revelou que: 1) a cepa de B. bovis apresenta baixa virulência, uma vez que os animais inoculados não apresentaram sinais clínicos da doença durante a reação vacinal; 2) a cepa de B. bigemina apresenta ainda um grau elevado de virulência pois, dos cinco novilhos inoculados, três apresentaram sintomatologia clínica embora tenham se recuperado espontaneamente da infecção; 3) ambas as cepas apresentaram alta antigenicidade pois todos os animais inoculados apresentaram sorologicamente positivos ao teste de imunofluorescência indireta específico e resistiram ao desafio homólogo com as respectivas cepas virulentas, enquanto nos grupos testemunha, todos os novilhos sofreram a doença aguda e, apesar de devidamente medicados, um morreu.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraKessler, Raul HenriqueSacco, Ana Maria S.Jesus, Elane F. deMadruga, Cláudio Roberto2014-04-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/14698Pesquisa Agropecuaria Brasileira; v.22, n.11/12, nov./dez. 1987; 1225-1230Pesquisa Agropecuária Brasileira; v.22, n.11/12, nov./dez. 1987; 1225-12301678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/14698/8383info:eu-repo/semantics/openAccess2014-10-15T17:17:19Zoai:ojs.seer.sct.embrapa.br:article/14698Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-10-15T17:17:19Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary test
Desenvolvimento de cepas vivas atenuadas de Babesia bovis e Babesia bigemina: teste preliminar
title Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary test
spellingShingle Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary test
Kessler, Raul Henrique
Babesiosis; vaccine; attenuation; bovine passage
babesiose; vacina; atenuação; passagem bovina
title_short Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary test
title_full Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary test
title_fullStr Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary test
title_full_unstemmed Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary test
title_sort Development of live attenuated strains of Babesia bovis and Babesia bigemina: preliminary test
author Kessler, Raul Henrique
author_facet Kessler, Raul Henrique
Sacco, Ana Maria S.
Jesus, Elane F. de
Madruga, Cláudio Roberto
author_role author
author2 Sacco, Ana Maria S.
Jesus, Elane F. de
Madruga, Cláudio Roberto
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Kessler, Raul Henrique
Sacco, Ana Maria S.
Jesus, Elane F. de
Madruga, Cláudio Roberto
dc.subject.por.fl_str_mv Babesiosis; vaccine; attenuation; bovine passage
babesiose; vacina; atenuação; passagem bovina
topic Babesiosis; vaccine; attenuation; bovine passage
babesiose; vacina; atenuação; passagem bovina
description With the objective of developing local strains of Babesia bovis and Babesia bigemina attenuated according to the Australian method, sixteen rapid passages of B. bovis in splenectomized calves and four slow passages of B. bigemina in intact calves were made. A preliminary test of the virulence and antigenicity of these strains in two-year old Hereford steers in the field showed that: 1) the B. bovis strain was of low virulence as the inoculated animals did not present clinical signs of the disease during the vaccine reaction; 2) the B. bigemina strain was still too virulent since three of five inoculated steers presented visible clinical signs, although all recovered spontaneously from the infection; 3) both strains were highly antigenic as all inoculated animals were positive for the indirect fluorescent antibody test and resisted the homologous challenge with the respective virulent strain, while all of the non-vaccinated controls became acutely ill and although properly medicated, one died.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-17
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/14698
url https://seer.sct.embrapa.br/index.php/pab/article/view/14698
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/14698/8383
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.22, n.11/12, nov./dez. 1987; 1225-1230
Pesquisa Agropecuária Brasileira; v.22, n.11/12, nov./dez. 1987; 1225-1230
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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