In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis

Detalhes bibliográficos
Autor(a) principal: Hoffmann, Lucia Vieira
Data de Publicação: 2000
Outros Autores: Vieira, Maria Lucia Carneiro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/5851
Resumo: Two Stylosanthes guianensis (Leguminosae) cultivars, Bandeirantes and Mineirão, were evaluated for their ability to form adventitious shoots in vitro and Agrobacterium susceptibility. To obtain regeneration, several explant sources were used and MS basal medium was supplemented with different concentrations of NAA and BAP. Shoot regeneration occurred in both cultivars from cotyledon and hypocotyl-derived tissues. Stylosanthes was susceptible to wild Agrobacterium strains, since tumor formation was induced. Transient expression of the uidA gene was observed on infected Stylosanthes tissues. Experiments on the effect of the antibiotic cefotaxime and tetracycline, used in transformation assays to Agrobacterium elimination, showed that cefotaxime (250 µg mL-1) has a tendency to reduce shoot regeneration in cv. Bandeirantes explants.
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spelling In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis Resposta in vitro e suscetibilidade ao Agrobacterium de duas cultivares de Stylosanthes guianensistissue culture; forage legume; plant transformation; transient expressioncultura de tecidos; leguminosa forrageira; transformação de plantas; expressão transienteTwo Stylosanthes guianensis (Leguminosae) cultivars, Bandeirantes and Mineirão, were evaluated for their ability to form adventitious shoots in vitro and Agrobacterium susceptibility. To obtain regeneration, several explant sources were used and MS basal medium was supplemented with different concentrations of NAA and BAP. Shoot regeneration occurred in both cultivars from cotyledon and hypocotyl-derived tissues. Stylosanthes was susceptible to wild Agrobacterium strains, since tumor formation was induced. Transient expression of the uidA gene was observed on infected Stylosanthes tissues. Experiments on the effect of the antibiotic cefotaxime and tetracycline, used in transformation assays to Agrobacterium elimination, showed that cefotaxime (250 µg mL-1) has a tendency to reduce shoot regeneration in cv. Bandeirantes explants.As cultivares Bandeirantes e Mineirão de Stylosanthes guianensis (Leguminosae) foram avaliadas quanto à capacidade de formação de brotos adventícios in vitro e quanto à suscetibilidade ao Agrobacterium. Para obter regeneração, foram utilizados vários tipos de explantes, e o meio basal MS foi suplementado com diferentes concentrações de ácido naftalenoacético (NAA) e 6-benzilanimopurina (BAP). Observou-se regeneração de brotos a partir de explantes cotiledonares e hipocotiledonares, nas duas cultivares. O Stylosanthes mostrou-se suscetível ao Agrobacterium selvagem, pois a formação de tumores foi induzida. Expressão transiente do gene uidA foi observada em tecidos infectados de Stylosanthes. Experimentos sobre a ação dos antibióticos cefotaxima e tetraciclina, usados em ensaios de transformação para eliminação do Agrobacterium, mostraram que a cefotaxima (250 µg mL-1) tende a reduzir a regeneração de brotos em explantes da cv. Bandeirantes.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraHoffmann, Lucia VieiraVieira, Maria Lucia Carneiro2000-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/5851Pesquisa Agropecuaria Brasileira; v.35, n.4, abr. 2000; 733-742Pesquisa Agropecuária Brasileira; v.35, n.4, abr. 2000; 733-7421678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/5851/2955info:eu-repo/semantics/openAccess2010-08-03T11:53:41Zoai:ojs.seer.sct.embrapa.br:article/5851Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2010-08-03T11:53:41Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis
Resposta in vitro e suscetibilidade ao Agrobacterium de duas cultivares de Stylosanthes guianensis
title In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis
spellingShingle In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis
Hoffmann, Lucia Vieira
tissue culture; forage legume; plant transformation; transient expression
cultura de tecidos; leguminosa forrageira; transformação de plantas; expressão transiente
title_short In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis
title_full In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis
title_fullStr In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis
title_full_unstemmed In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis
title_sort In vitro response and Agrobacterium susceptibility of two cultivars of Stylosanthes guianensis
author Hoffmann, Lucia Vieira
author_facet Hoffmann, Lucia Vieira
Vieira, Maria Lucia Carneiro
author_role author
author2 Vieira, Maria Lucia Carneiro
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Hoffmann, Lucia Vieira
Vieira, Maria Lucia Carneiro
dc.subject.por.fl_str_mv tissue culture; forage legume; plant transformation; transient expression
cultura de tecidos; leguminosa forrageira; transformação de plantas; expressão transiente
topic tissue culture; forage legume; plant transformation; transient expression
cultura de tecidos; leguminosa forrageira; transformação de plantas; expressão transiente
description Two Stylosanthes guianensis (Leguminosae) cultivars, Bandeirantes and Mineirão, were evaluated for their ability to form adventitious shoots in vitro and Agrobacterium susceptibility. To obtain regeneration, several explant sources were used and MS basal medium was supplemented with different concentrations of NAA and BAP. Shoot regeneration occurred in both cultivars from cotyledon and hypocotyl-derived tissues. Stylosanthes was susceptible to wild Agrobacterium strains, since tumor formation was induced. Transient expression of the uidA gene was observed on infected Stylosanthes tissues. Experiments on the effect of the antibiotic cefotaxime and tetracycline, used in transformation assays to Agrobacterium elimination, showed that cefotaxime (250 µg mL-1) has a tendency to reduce shoot regeneration in cv. Bandeirantes explants.
publishDate 2000
dc.date.none.fl_str_mv 2000-04-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5851
url https://seer.sct.embrapa.br/index.php/pab/article/view/5851
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5851/2955
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.35, n.4, abr. 2000; 733-742
Pesquisa Agropecuária Brasileira; v.35, n.4, abr. 2000; 733-742
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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