Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken livers
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/17734 |
Resumo: | Total copper, iron and zinc contents of male Shaver-Starbro pullet chicken livers were determined by atomic absorption spectrophotometry. In applying this method to dry ashed samples of liver tissue from 32 normal animals (1,34 mean body weight) the following values were obtained: 14,4 ± 0,85 for copper, 283,3 ± 19,78 for iron and 130,4 ± 4,21 for zinc (mean ± standard deviation ppm refered to tissue dry weight). Recovery tests were carried out to check the occurrence of metal interferences and contamination as well as analytical losses. Very good recoveries were obtained both in the absence of organic matter (101,3% for Cu, 102,9% for Fe and 99,0% for Zn) and in the presence of chicken powder (92,9% for Cu, 97,7% for Fe and 98,0% por Zn). The results of these tests excluded the eventual contamination of the samples, glassware and reagents used. There were no evidences of any spectral interference by other elements normally present in large amounts in the ashes, such as, sodium, potassium, calcium and phosphorus. Duplicate error of analysis indicated the good reproducibility of the method. Atomic absorption spectrophotometry quantitative analysis of the metalic elements occurring in animal tissues and body fluids is recommended in view of its sensitivity, versatility and convenience. The advantages have been emphasized particularly for surveys on trace elements deficiences occurring in bovine and other animal species, which requires a convenient handling of large number of samples. The method offers, even so, the advantage of a direct analytical tool, free of troublesome and time consuming fractionation steps. The simplicity of the procedure and the reproducibility and reliability of the results obtained lead to the adaption of this method for analytical studies of bovine and avian mineral metabolism undertaken in this laboratory. |
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Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken liversAnálise de materiais biológicos por espectrofotometria de absorção atômica. I. Determinação do cobre, ferro e zinco totais no fígado de aves normaisTotal copper, iron and zinc contents of male Shaver-Starbro pullet chicken livers were determined by atomic absorption spectrophotometry. In applying this method to dry ashed samples of liver tissue from 32 normal animals (1,34 mean body weight) the following values were obtained: 14,4 ± 0,85 for copper, 283,3 ± 19,78 for iron and 130,4 ± 4,21 for zinc (mean ± standard deviation ppm refered to tissue dry weight). Recovery tests were carried out to check the occurrence of metal interferences and contamination as well as analytical losses. Very good recoveries were obtained both in the absence of organic matter (101,3% for Cu, 102,9% for Fe and 99,0% for Zn) and in the presence of chicken powder (92,9% for Cu, 97,7% for Fe and 98,0% por Zn). The results of these tests excluded the eventual contamination of the samples, glassware and reagents used. There were no evidences of any spectral interference by other elements normally present in large amounts in the ashes, such as, sodium, potassium, calcium and phosphorus. Duplicate error of analysis indicated the good reproducibility of the method. Atomic absorption spectrophotometry quantitative analysis of the metalic elements occurring in animal tissues and body fluids is recommended in view of its sensitivity, versatility and convenience. The advantages have been emphasized particularly for surveys on trace elements deficiences occurring in bovine and other animal species, which requires a convenient handling of large number of samples. The method offers, even so, the advantage of a direct analytical tool, free of troublesome and time consuming fractionation steps. The simplicity of the procedure and the reproducibility and reliability of the results obtained lead to the adaption of this method for analytical studies of bovine and avian mineral metabolism undertaken in this laboratory.São apresentados os resultados obtidos na determinação do cobre, ferro e zinco, em fígado seco de frangos de corte da linhagem Shaver-Starbro, utilizando a espectrofotometria de absorção atômica como método analítico. Também são analisadas as possibilidades de estudo do metabolismo destes elementos em aves, sendo indicados alguns campos de estudo do metabolismo mineral em que as aves se prestam admiravelmente à experimentação. Os valores encontrados nos fígados analisados (expressos em ppm no tecido seco), foram 14,4 ± 0,85 ppm para o cobre; 283,3 ± 19,78 ppm para o ferro e 130,4 ± 4,21 ppm para o zinco. Estes valores estão dentro dos limites indicados na escassa literatura existente a respeito. O método utilizado foi controlado para a verificação da ocorrência de interferências, de perdas, contaminação operacional e reprodutibilidade analítica, pelas provas do erro de duplicatas e de recuperação dos três elementos, na presença e na ausência de matéria orgânica. Tais provas forneceram resultados significativos, sendo que a recuperação dos três elementos na ausência de matéria orgânica foi de 101,3% para o cobre, 102,9% para o ferro e 99,0% para o zinco; na presença de matéria orgânica os resultados foram: 92,9%, 97,7% e 98,0%, respectivamente. Não foi verificado ocorrerem interferências por parte de outros elementos presentes na cinza em grande quantidade, como o sódio, o potássio, o cálcio e o fósforo. A prova do erro de duplicatas indicou grande uniformidade dos resultados obtidos. A análise dos elementos presentes em tecidos e líquidos biológicos por absorção atômica se recomenda pela versatilidade, sensibilidade e comodidade para o manuseio de grande número de amostras e, principalmente, pela eliminação dos métodos de fracionamento dos componentes da mistura.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraGuimarães, Jorge AlmeidaGarcia, Eloi de SouzaUbatuba, Fernando Braga2014-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/17734Pesquisa Agropecuaria Brasileira; v.6, n.1, 1971: Série Agronomia e Veterinária; 45-51Pesquisa Agropecuária Brasileira; v.6, n.1, 1971: Série Agronomia e Veterinária; 45-511678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/17734/11899info:eu-repo/semantics/openAccess2014-04-15T18:36:02Zoai:ojs.seer.sct.embrapa.br:article/17734Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-15T18:36:02Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken livers Análise de materiais biológicos por espectrofotometria de absorção atômica. I. Determinação do cobre, ferro e zinco totais no fígado de aves normais |
title |
Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken livers |
spellingShingle |
Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken livers Guimarães, Jorge Almeida |
title_short |
Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken livers |
title_full |
Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken livers |
title_fullStr |
Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken livers |
title_full_unstemmed |
Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken livers |
title_sort |
Analysis of biological materials by the atomic absorption spectrophotometry. I. Determination of total copper, iron and zinc in chicken livers |
author |
Guimarães, Jorge Almeida |
author_facet |
Guimarães, Jorge Almeida Garcia, Eloi de Souza Ubatuba, Fernando Braga |
author_role |
author |
author2 |
Garcia, Eloi de Souza Ubatuba, Fernando Braga |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Guimarães, Jorge Almeida Garcia, Eloi de Souza Ubatuba, Fernando Braga |
dc.subject.none.fl_str_mv |
|
description |
Total copper, iron and zinc contents of male Shaver-Starbro pullet chicken livers were determined by atomic absorption spectrophotometry. In applying this method to dry ashed samples of liver tissue from 32 normal animals (1,34 mean body weight) the following values were obtained: 14,4 ± 0,85 for copper, 283,3 ± 19,78 for iron and 130,4 ± 4,21 for zinc (mean ± standard deviation ppm refered to tissue dry weight). Recovery tests were carried out to check the occurrence of metal interferences and contamination as well as analytical losses. Very good recoveries were obtained both in the absence of organic matter (101,3% for Cu, 102,9% for Fe and 99,0% for Zn) and in the presence of chicken powder (92,9% for Cu, 97,7% for Fe and 98,0% por Zn). The results of these tests excluded the eventual contamination of the samples, glassware and reagents used. There were no evidences of any spectral interference by other elements normally present in large amounts in the ashes, such as, sodium, potassium, calcium and phosphorus. Duplicate error of analysis indicated the good reproducibility of the method. Atomic absorption spectrophotometry quantitative analysis of the metalic elements occurring in animal tissues and body fluids is recommended in view of its sensitivity, versatility and convenience. The advantages have been emphasized particularly for surveys on trace elements deficiences occurring in bovine and other animal species, which requires a convenient handling of large number of samples. The method offers, even so, the advantage of a direct analytical tool, free of troublesome and time consuming fractionation steps. The simplicity of the procedure and the reproducibility and reliability of the results obtained lead to the adaption of this method for analytical studies of bovine and avian mineral metabolism undertaken in this laboratory. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-15 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/17734 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/17734 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/17734/11899 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.6, n.1, 1971: Série Agronomia e Veterinária; 45-51 Pesquisa Agropecuária Brasileira; v.6, n.1, 1971: Série Agronomia e Veterinária; 45-51 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416650739941376 |