Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cotton

Detalhes bibliográficos
Autor(a) principal: González-Benito, Maria Elena
Data de Publicação: 1998
Outros Autores: Carvalho, Julita María Frota-Chagas, Pérez, César
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/4820
Resumo: The aim of the present work was to explore the feasibility of seed cryopreservation as a tool for cotton (Gossypium hirsutum L.) genetic resources conservation. Therefore, seeds of five cotton cultivars (CNPA 4M, CNPA 5M, CNPA Precoce 1, CNPA Precoce 2, Coker 312) were subjected to desiccation and/or immersion in liquid nitrogen. Germination was not affected by any of the treatments in cultivars CNPA 4M, CNPA Precoce 1 and Coker 312. Desiccation from 9.8% and 15.6% moisture contents to 2.8% and 3.0% decreased seed viability in CNPA 5M and CNPA Precoce 2. However, germination of desiccated seeds improved after freezing. Embryonic axes of CNPA Precoce 2 and Coker 312 withstood cryopreservation when their moisture content was 19.4% or lower. Humidification did not have any effect on germination of Coker 312 seeds; on the other hand, it improved viability of CNPA Precoce 2 desiccated seeds.
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spelling Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cottonEfeito da dessecação e crioconservação na germinação de eixos embrionários e sementes de algodãoGossypium hirsutum; liquid nitrogenGossypium hirsutum; nitrogênio líquidoThe aim of the present work was to explore the feasibility of seed cryopreservation as a tool for cotton (Gossypium hirsutum L.) genetic resources conservation. Therefore, seeds of five cotton cultivars (CNPA 4M, CNPA 5M, CNPA Precoce 1, CNPA Precoce 2, Coker 312) were subjected to desiccation and/or immersion in liquid nitrogen. Germination was not affected by any of the treatments in cultivars CNPA 4M, CNPA Precoce 1 and Coker 312. Desiccation from 9.8% and 15.6% moisture contents to 2.8% and 3.0% decreased seed viability in CNPA 5M and CNPA Precoce 2. However, germination of desiccated seeds improved after freezing. Embryonic axes of CNPA Precoce 2 and Coker 312 withstood cryopreservation when their moisture content was 19.4% or lower. Humidification did not have any effect on germination of Coker 312 seeds; on the other hand, it improved viability of CNPA Precoce 2 desiccated seeds.O objetivo do presente trabalho foi explorar a viabilidade da crioconservação de sementes como um método de conservação do patrimônio genético do algodão. Sementes de cinco cultivares de algodão (Gossypium hirsutum L.) (CNPA 4M, CNPA 5M, CNPA Precoce 1, CNPA Precoce 2 e Coker 312) foram submetidas a dessecação e/ou imersão em nitrogênio líquido. A germinação não foi afetada por nenhum dos tratamentos nas cultivares CNPA 4M, CNPA Precoce 1 e Coker 312. A viabilidade das sementes das cultivares CNPA 5M e CNPA Precoce 2 foi reduzida quando estas sementes foram dessecadas e o teor de umidade baixou de 9,8 e 15,6% para 2,8 e 3%, respectivamente. Entretanto, a germinação das sementes dessecadas foi aumentada depois da imersão em nitrogênio líquido. Os eixos embrionários das cultivares CNPA Precoce 2 e Coker 312 resistiram a crioconservação quando seu teor de umidade foi igual ou menor do que 19,4%. O umedecimento das sementes da cultivar Coker 312 não teve nenhum efeito na germinação, enquanto que a viabilidade das sementes da cultivar CNPA Precoce 2 foi aumentada pela dessecação.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraGonzález-Benito, Maria ElenaCarvalho, Julita María Frota-ChagasPérez, César1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/4820Pesquisa Agropecuaria Brasileira; v.33, n.1, jan. 1998; 17-20Pesquisa Agropecuária Brasileira; v.33, n.1, jan. 1998; 17-201678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/4820/6926info:eu-repo/semantics/openAccess2014-11-07T16:51:24Zoai:ojs.seer.sct.embrapa.br:article/4820Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-11-07T16:51:24Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cotton
Efeito da dessecação e crioconservação na germinação de eixos embrionários e sementes de algodão
title Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cotton
spellingShingle Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cotton
González-Benito, Maria Elena
Gossypium hirsutum; liquid nitrogen
Gossypium hirsutum; nitrogênio líquido
title_short Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cotton
title_full Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cotton
title_fullStr Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cotton
title_full_unstemmed Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cotton
title_sort Effect of desiccation and cryopreservation on the germination of embryonic axes and seeds of cotton
author González-Benito, Maria Elena
author_facet González-Benito, Maria Elena
Carvalho, Julita María Frota-Chagas
Pérez, César
author_role author
author2 Carvalho, Julita María Frota-Chagas
Pérez, César
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv González-Benito, Maria Elena
Carvalho, Julita María Frota-Chagas
Pérez, César
dc.subject.por.fl_str_mv Gossypium hirsutum; liquid nitrogen
Gossypium hirsutum; nitrogênio líquido
topic Gossypium hirsutum; liquid nitrogen
Gossypium hirsutum; nitrogênio líquido
description The aim of the present work was to explore the feasibility of seed cryopreservation as a tool for cotton (Gossypium hirsutum L.) genetic resources conservation. Therefore, seeds of five cotton cultivars (CNPA 4M, CNPA 5M, CNPA Precoce 1, CNPA Precoce 2, Coker 312) were subjected to desiccation and/or immersion in liquid nitrogen. Germination was not affected by any of the treatments in cultivars CNPA 4M, CNPA Precoce 1 and Coker 312. Desiccation from 9.8% and 15.6% moisture contents to 2.8% and 3.0% decreased seed viability in CNPA 5M and CNPA Precoce 2. However, germination of desiccated seeds improved after freezing. Embryonic axes of CNPA Precoce 2 and Coker 312 withstood cryopreservation when their moisture content was 19.4% or lower. Humidification did not have any effect on germination of Coker 312 seeds; on the other hand, it improved viability of CNPA Precoce 2 desiccated seeds.
publishDate 1998
dc.date.none.fl_str_mv 1998-01-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4820
url https://seer.sct.embrapa.br/index.php/pab/article/view/4820
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4820/6926
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.33, n.1, jan. 1998; 17-20
Pesquisa Agropecuária Brasileira; v.33, n.1, jan. 1998; 17-20
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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