A nondigestion extraction method for boron in plant leaves

Detalhes bibliográficos
Autor(a) principal: Correa, Antonio E.
Data de Publicação: 2014
Outros Autores: Miyazawa, Mario, Pavan, Marcos A.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/16303
Resumo: A nondigestion procedure using HCl for the extraction of B in plant tissues is described. Plant materials were treated with 1NHCl, heated at 80°C for 15 minutes, shaken at 250 rpm for 60 minutes, and filtered. Then, 5 ml of the extracted solution was treated with 100 mg of activated charcoal, shaken at 250 rpm for 15 minutes, and refiltered. Boron was determined by the colorimetric azomethine-H method. The results were compared with those obtained by dry-ashing method. B contents were determined by the colorimetric method with azomethine-H. The results have shown that both methods extracted identical quantities of B. B adsorption by activated-charcoal in an acid-medium was minimum. The presence of activated charcoal in the nondigested leaves extract carried out the complete adsorption of the soluble substances interfering with B colorimetric determination at 420 nm. The mean B extraction accuracy by the proposed method was 1.54 percent.
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spelling A nondigestion extraction method for boron in plant leavesMétodo de não-digestão para extração de boro em folhas de plantasplant analysis; activated charcoal; organic interference; azomethine-Hanálise de plantas; carvão ativado; interferências orgânicas; azomethina-HA nondigestion procedure using HCl for the extraction of B in plant tissues is described. Plant materials were treated with 1NHCl, heated at 80°C for 15 minutes, shaken at 250 rpm for 60 minutes, and filtered. Then, 5 ml of the extracted solution was treated with 100 mg of activated charcoal, shaken at 250 rpm for 15 minutes, and refiltered. Boron was determined by the colorimetric azomethine-H method. The results were compared with those obtained by dry-ashing method. B contents were determined by the colorimetric method with azomethine-H. The results have shown that both methods extracted identical quantities of B. B adsorption by activated-charcoal in an acid-medium was minimum. The presence of activated charcoal in the nondigested leaves extract carried out the complete adsorption of the soluble substances interfering with B colorimetric determination at 420 nm. The mean B extraction accuracy by the proposed method was 1.54 percent.É descrito um procedimento analítico para o diagnóstico do estado nutricional de B em folhas de plantas por um método de extração sem digestão com a solução de HCl. A extração consistiu na adição de 20 ml de HCl 1N em tubos de ensaio contendo 400 mg do material vegetal, seguido por aquecimento a 80°C por 15 minutos, agitação por 60 minutos a 250 rpm e filtração. A seguir, transferiu- se uma alíquota de 5 ml do filtrado para tubos de ensaio de 200 ml, adicionaram-se 100 mg de carvão ativado, agitado por 15 minutos a 250 rpm e refiltrado. Os resultados foram comparados com aqueles obtidos por digestão seca. Os teores de B foram determinados pelo método colorimétrico com azomethina-H. Os resultados evidenciaram que ambos os métodos extraíram quantidades idênticas de B. A adsorção do B pelo carvão ativado em um meio-ácido foi desprezível. A presença do carvão ativado no extrato de folhas sem digestão promoveu a completa adsorção das substâncias solúveis interferentes na determinação colorimétrica de B a 420 nm, A precisão média da extração de B pelo método proposto foi de 1,54%.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCorrea, Antonio E.Miyazawa, MarioPavan, Marcos A.2014-04-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/16303Pesquisa Agropecuaria Brasileira; v.20, n.10, out. 1985; 1213-1216Pesquisa Agropecuária Brasileira; v.20, n.10, out. 1985; 1213-12161678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/16303/10500info:eu-repo/semantics/openAccess2014-04-16T19:33:06Zoai:ojs.seer.sct.embrapa.br:article/16303Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-16T19:33:06Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv A nondigestion extraction method for boron in plant leaves
Método de não-digestão para extração de boro em folhas de plantas
title A nondigestion extraction method for boron in plant leaves
spellingShingle A nondigestion extraction method for boron in plant leaves
Correa, Antonio E.
plant analysis; activated charcoal; organic interference; azomethine-H
análise de plantas; carvão ativado; interferências orgânicas; azomethina-H
title_short A nondigestion extraction method for boron in plant leaves
title_full A nondigestion extraction method for boron in plant leaves
title_fullStr A nondigestion extraction method for boron in plant leaves
title_full_unstemmed A nondigestion extraction method for boron in plant leaves
title_sort A nondigestion extraction method for boron in plant leaves
author Correa, Antonio E.
author_facet Correa, Antonio E.
Miyazawa, Mario
Pavan, Marcos A.
author_role author
author2 Miyazawa, Mario
Pavan, Marcos A.
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Correa, Antonio E.
Miyazawa, Mario
Pavan, Marcos A.
dc.subject.por.fl_str_mv plant analysis; activated charcoal; organic interference; azomethine-H
análise de plantas; carvão ativado; interferências orgânicas; azomethina-H
topic plant analysis; activated charcoal; organic interference; azomethine-H
análise de plantas; carvão ativado; interferências orgânicas; azomethina-H
description A nondigestion procedure using HCl for the extraction of B in plant tissues is described. Plant materials were treated with 1NHCl, heated at 80°C for 15 minutes, shaken at 250 rpm for 60 minutes, and filtered. Then, 5 ml of the extracted solution was treated with 100 mg of activated charcoal, shaken at 250 rpm for 15 minutes, and refiltered. Boron was determined by the colorimetric azomethine-H method. The results were compared with those obtained by dry-ashing method. B contents were determined by the colorimetric method with azomethine-H. The results have shown that both methods extracted identical quantities of B. B adsorption by activated-charcoal in an acid-medium was minimum. The presence of activated charcoal in the nondigested leaves extract carried out the complete adsorption of the soluble substances interfering with B colorimetric determination at 420 nm. The mean B extraction accuracy by the proposed method was 1.54 percent.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-16
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/16303
url https://seer.sct.embrapa.br/index.php/pab/article/view/16303
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/16303/10500
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.20, n.10, out. 1985; 1213-1216
Pesquisa Agropecuária Brasileira; v.20, n.10, out. 1985; 1213-1216
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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