Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil

Detalhes bibliográficos
Autor(a) principal: Rivas, Eliana Borges
Data de Publicação: 2016
Outros Autores: Bôdi, Estevão Cardoso de Almeida, Harakava, Ricardo, Gregori, Fabio, Gonçalves, Marcos Cesar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/20813
Resumo: The objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus – LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT‑PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV‑infected lilies and nine fragments of 600 nt from LSV‑infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV).
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spelling Occurrence and molecular analysis of quarantine virus in lily cultivation areas in BrazilOcorrência e análise molecular de vírus quarentenário em áreas de cultivo de lírios no BrasilLilium; Lily mottle virus; Lily symptomless virus; phylogeny; recombination analysis; RT‑PCRLilium; Lily mottle virus; Lily symptomless virus; filogenia; análise de recombinação; RT‑PCRThe objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus – LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT‑PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV‑infected lilies and nine fragments of 600 nt from LSV‑infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV).O objetivo deste trabalho foi descrever a ocorrência do Tulip breaking virus (TBV, sinonímia Lily mottle virus – LMoV) quarentenário e do Lily symptomless virus (LSV) e suas análises moleculares, para fornecer dados para a remoção do TBV da lista brasileira de pragas quarentenárias A1, pois este vírus está disseminado pelos principais cultivos comerciais de lírio no Brasil. A ocorrência desses vírus foi detectada em 12 regiões produtoras, por meio da reação de transcrição reversa multiplex (RT‑PCR), com uso de iniciadores específicos para os genes das respectivas capas proteicas (CP). Foram sequenciados oito fragmentos de 800 nucleotídeos (nt), obtidos de lírios infectados com LMoV, e nove fragmentos de 600 nt, obtidos de lírios infectados com LSV. A reconstrução da árvore filogenética mostrou um ramo robusto que continha as sequências brasileiras de LMoV, outros isolados de LMoV, TBV e Tulip band breaking virus, o que indica que todos são isolados de LMoV, embora agrupados em dois subgrupos. A análise filogenética também mostrou um ramo robusto que suporta todas as sequências brasileiras e outras de LSV, com exceção de um isolado japonês de LSV. Análises de recombinação também mostraram um isolado recombinante de LMoV, enquanto nenhum evento de recombinação foi detectado em LSV. Lily mottle virus é prevalente em cultivos de lírios no Brasil, em infecções simples e mistas com LSV ou Cucumber mosaic virus (CMV).Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraRivas, Eliana BorgesBôdi, Estevão Cardoso de AlmeidaHarakava, RicardoGregori, FabioGonçalves, Marcos Cesar2016-08-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/20813Pesquisa Agropecuaria Brasileira; v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária; 615-622Pesquisa Agropecuária Brasileira; v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária; 615-6221678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/20813/13325https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20813/12979https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20813/12980https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20813/12981https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20813/12982Direitos autorais 2016 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2016-08-10T14:29:22Zoai:ojs.seer.sct.embrapa.br:article/20813Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2016-08-10T14:29:22Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
Ocorrência e análise molecular de vírus quarentenário em áreas de cultivo de lírios no Brasil
title Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
spellingShingle Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
Rivas, Eliana Borges
Lilium; Lily mottle virus; Lily symptomless virus; phylogeny; recombination analysis; RT‑PCR
Lilium; Lily mottle virus; Lily symptomless virus; filogenia; análise de recombinação; RT‑PCR
title_short Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
title_full Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
title_fullStr Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
title_full_unstemmed Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
title_sort Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil
author Rivas, Eliana Borges
author_facet Rivas, Eliana Borges
Bôdi, Estevão Cardoso de Almeida
Harakava, Ricardo
Gregori, Fabio
Gonçalves, Marcos Cesar
author_role author
author2 Bôdi, Estevão Cardoso de Almeida
Harakava, Ricardo
Gregori, Fabio
Gonçalves, Marcos Cesar
author2_role author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Rivas, Eliana Borges
Bôdi, Estevão Cardoso de Almeida
Harakava, Ricardo
Gregori, Fabio
Gonçalves, Marcos Cesar
dc.subject.por.fl_str_mv Lilium; Lily mottle virus; Lily symptomless virus; phylogeny; recombination analysis; RT‑PCR
Lilium; Lily mottle virus; Lily symptomless virus; filogenia; análise de recombinação; RT‑PCR
topic Lilium; Lily mottle virus; Lily symptomless virus; phylogeny; recombination analysis; RT‑PCR
Lilium; Lily mottle virus; Lily symptomless virus; filogenia; análise de recombinação; RT‑PCR
description The objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus – LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT‑PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV‑infected lilies and nine fragments of 600 nt from LSV‑infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV).
publishDate 2016
dc.date.none.fl_str_mv 2016-08-03
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/20813
url https://seer.sct.embrapa.br/index.php/pab/article/view/20813
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/20813/13325
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20813/12979
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20813/12980
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20813/12981
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/20813/12982
dc.rights.driver.fl_str_mv Direitos autorais 2016 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2016 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária; 615-622
Pesquisa Agropecuária Brasileira; v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária; 615-622
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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