Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE

Detalhes bibliográficos
Autor(a) principal: Lacava, Paulo Teixeira
Data de Publicação: 2006
Outros Autores: Andreote, Fernando Dini, Araújo, Welington Luiz, Azevedo, João Lúcio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7185
Resumo: The aim of this work was to characterize endophytic bacterial community of assintomatic (escape) and Citrus Variegated Chlorosis (CVC)-affected citrus plants using isolation in culture medium, denaturing gradient gel electrophoresis (DGGE) technique and Methylobacterium mesophilicum as well as Xylella fastidiosa specific PCR, allowing to assess this community and its interactions with CVC. The study of bacterial community by DGGE analysis allowed the detection of X. fastidiosa, as well as Klebsiella sp. e Acinetobacter sp., which were not detected previously. Curtobacterium sp., Pseudomonas sp., Enterobacter sp. and Bacillus spp. were also observed as endophyte in citrus plants. Using specific primers Methylobacterium mesophilicum and X. fastidiosa were observed, reinforcing that these bacteria could interact inside the host plant.
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spelling Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGECaracterização da comunidade bacteriana endofítica de citros por isolamento, PCR específico e DGGECitrus sinensis; Xylella fastidiosa; Methylobacterium; endophytic bacteriaCitrus sinensis; Xylella fastidiosa; Methylobacterium; bactérias endofíticasThe aim of this work was to characterize endophytic bacterial community of assintomatic (escape) and Citrus Variegated Chlorosis (CVC)-affected citrus plants using isolation in culture medium, denaturing gradient gel electrophoresis (DGGE) technique and Methylobacterium mesophilicum as well as Xylella fastidiosa specific PCR, allowing to assess this community and its interactions with CVC. The study of bacterial community by DGGE analysis allowed the detection of X. fastidiosa, as well as Klebsiella sp. e Acinetobacter sp., which were not detected previously. Curtobacterium sp., Pseudomonas sp., Enterobacter sp. and Bacillus spp. were also observed as endophyte in citrus plants. Using specific primers Methylobacterium mesophilicum and X. fastidiosa were observed, reinforcing that these bacteria could interact inside the host plant.O objetivo deste trabalho foi caracterizar a comunidade bacteriana endofítica de plantas assintomáticas (escapes) e afetadas pela clorose variegada dos citros (CVC) por meio de isolamento em meio de cultura, técnica de gradiente desnaturante em gel de eletroforese (DGGE) e detecção de Methylobacterium mesophilicum e Xyllela fastidiosa por meio de PCR específico, para estudar esta comunidade e sua relação com a ocorrência da CVC. A análise da comunidade bacteriana via DGGE permitiu a detecção de X. fastidiosa, bem como Klebsiella sp. e Acinetobacter sp. como endófitos de citros. Foram observados também Curtobacterium sp., Pseudomonas sp., Enterobacter sp. e Bacillus spp. Utilizando primers específicos, Methylobacterium mesophilicum e X. fastidiosa também foram observadas, reforçando hipóteses de que estas bactérias podem estar interagindo no interior da planta hospedeira.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraLacava, Paulo TeixeiraAndreote, Fernando DiniAraújo, Welington LuizAzevedo, João Lúcio2006-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7185Pesquisa Agropecuaria Brasileira; v.41, n.4, abr. 2006; 637-642Pesquisa Agropecuária Brasileira; v.41, n.4, abr. 2006; 637-6421678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7185/4230info:eu-repo/semantics/openAccess2014-09-29T17:51:36Zoai:ojs.seer.sct.embrapa.br:article/7185Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-29T17:51:36Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE
Caracterização da comunidade bacteriana endofítica de citros por isolamento, PCR específico e DGGE
title Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE
spellingShingle Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE
Lacava, Paulo Teixeira
Citrus sinensis; Xylella fastidiosa; Methylobacterium; endophytic bacteria
Citrus sinensis; Xylella fastidiosa; Methylobacterium; bactérias endofíticas
title_short Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE
title_full Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE
title_fullStr Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE
title_full_unstemmed Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE
title_sort Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE
author Lacava, Paulo Teixeira
author_facet Lacava, Paulo Teixeira
Andreote, Fernando Dini
Araújo, Welington Luiz
Azevedo, João Lúcio
author_role author
author2 Andreote, Fernando Dini
Araújo, Welington Luiz
Azevedo, João Lúcio
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Lacava, Paulo Teixeira
Andreote, Fernando Dini
Araújo, Welington Luiz
Azevedo, João Lúcio
dc.subject.por.fl_str_mv Citrus sinensis; Xylella fastidiosa; Methylobacterium; endophytic bacteria
Citrus sinensis; Xylella fastidiosa; Methylobacterium; bactérias endofíticas
topic Citrus sinensis; Xylella fastidiosa; Methylobacterium; endophytic bacteria
Citrus sinensis; Xylella fastidiosa; Methylobacterium; bactérias endofíticas
description The aim of this work was to characterize endophytic bacterial community of assintomatic (escape) and Citrus Variegated Chlorosis (CVC)-affected citrus plants using isolation in culture medium, denaturing gradient gel electrophoresis (DGGE) technique and Methylobacterium mesophilicum as well as Xylella fastidiosa specific PCR, allowing to assess this community and its interactions with CVC. The study of bacterial community by DGGE analysis allowed the detection of X. fastidiosa, as well as Klebsiella sp. e Acinetobacter sp., which were not detected previously. Curtobacterium sp., Pseudomonas sp., Enterobacter sp. and Bacillus spp. were also observed as endophyte in citrus plants. Using specific primers Methylobacterium mesophilicum and X. fastidiosa were observed, reinforcing that these bacteria could interact inside the host plant.
publishDate 2006
dc.date.none.fl_str_mv 2006-04-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7185
url https://seer.sct.embrapa.br/index.php/pab/article/view/7185
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7185/4230
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.41, n.4, abr. 2006; 637-642
Pesquisa Agropecuária Brasileira; v.41, n.4, abr. 2006; 637-642
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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