Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherry

Detalhes bibliográficos
Autor(a) principal: Lopes, Ricardo
Data de Publicação: 2002
Outros Autores: Bruckner, Claudio Horst, Lopes, Maria Teresa Gomes
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6322
Resumo: The West Indian Cherry (Malpighia emarginata DC.) is a crop in expansion in Brazil mainly due to high fruit vitamin C content. However, there is still little information about the species. The objective of this study was to determine the activity and the polymorphism of isozymes from a collection of West Indian Cherry plants. IDH, MDH, EST, POD, ACP, GOT, PGM and PGI isozymes were studied. Starch gel horizontal electrophoresis and different gel/electrode buffers were used. Number of loci and alleles involved in genetic control of these isozymes were identified. IDH was dimeric and had one locus with two alleles. PGM was monomeric and had two loci, with two and four alleles. POD and EST were monomeric and had one and two loci, respectively, all with two alleles each. MDH had two polymorphic loci, one which was monomeric and one dimeric, both with two alleles each. GOT was polymorphic but its inheritance and genetic control was not determined. Polymorphisms of IDH, MDH, POD, EST, GOT and PGM showed potential as genetic markers in West Indian Cherry in studies of conservation and breeding.
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spelling Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherryPolimorfismo isozimático e potencial de utilização das isozimas como marcadores genéticos em aceroleiraMalpighia emarginata; genetic variation; enzymatic analysis; genetic inheritanceMalpighia emarginata; variação genética; análise enzimática; herança genéticaThe West Indian Cherry (Malpighia emarginata DC.) is a crop in expansion in Brazil mainly due to high fruit vitamin C content. However, there is still little information about the species. The objective of this study was to determine the activity and the polymorphism of isozymes from a collection of West Indian Cherry plants. IDH, MDH, EST, POD, ACP, GOT, PGM and PGI isozymes were studied. Starch gel horizontal electrophoresis and different gel/electrode buffers were used. Number of loci and alleles involved in genetic control of these isozymes were identified. IDH was dimeric and had one locus with two alleles. PGM was monomeric and had two loci, with two and four alleles. POD and EST were monomeric and had one and two loci, respectively, all with two alleles each. MDH had two polymorphic loci, one which was monomeric and one dimeric, both with two alleles each. GOT was polymorphic but its inheritance and genetic control was not determined. Polymorphisms of IDH, MDH, POD, EST, GOT and PGM showed potential as genetic markers in West Indian Cherry in studies of conservation and breeding.A aceroleira (Malpighia emarginata DC.) é uma cultura em expansão no Brasil, principalmente, por causa do elevado teor de vitamina C de seus frutos; contudo, ainda são escassas as informações sobre a espécie. Este trabalho teve o objetivo de estudar a atividade e o polimorfismo de alguns sistemas isozimáticos de uma coleção de aceroleiras. Foram estudadas as isozimas IDH, MDH, EST, ACP, GOT, PGM, PGI e POD. Utilizou-se o sistema de eletroforese horizontal em gel de amido e diferentes tampões de gel e cuba. Identificou-se o número de locos e alelos envolvidos no controle genético das enzimas. IDH demonstrou ser dimérica, e foi constatado um loco e dois alelos. PGM apresentou dois locos diméricos, com dois e quatro alelos. EST e POD demonstraram ser monoméricas, com um e dois locos, respectivamente, todos com dois alelos. MDH apresentou um loco onde o seu comportamento é monomérico e outro dimérico, ambos com dois alelos. GOT foi polimórfico mas não foi possível inferir sobre seu controle genético. O polimorfismo revelado pelos sistemas IDH, MDH, POD, EST, GOT e PGM demonstra o potencial de utilização das isozimas como marcadores genéticos em estudos de conservação e melhoramento de aceroleira.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraLopes, RicardoBruckner, Claudio HorstLopes, Maria Teresa Gomes2002-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6322Pesquisa Agropecuaria Brasileira; v.37, n.2, fev. 2002; 151-158Pesquisa Agropecuária Brasileira; v.37, n.2, fev. 2002; 151-1581678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6322/3379info:eu-repo/semantics/openAccess2014-07-21T19:12:57Zoai:ojs.seer.sct.embrapa.br:article/6322Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-21T19:12:57Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherry
Polimorfismo isozimático e potencial de utilização das isozimas como marcadores genéticos em aceroleira
title Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherry
spellingShingle Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherry
Lopes, Ricardo
Malpighia emarginata; genetic variation; enzymatic analysis; genetic inheritance
Malpighia emarginata; variação genética; análise enzimática; herança genética
title_short Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherry
title_full Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherry
title_fullStr Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherry
title_full_unstemmed Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherry
title_sort Isozyme polymorphism and potential of utilization of isozymes as genetics markers in West Indian cherry
author Lopes, Ricardo
author_facet Lopes, Ricardo
Bruckner, Claudio Horst
Lopes, Maria Teresa Gomes
author_role author
author2 Bruckner, Claudio Horst
Lopes, Maria Teresa Gomes
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Lopes, Ricardo
Bruckner, Claudio Horst
Lopes, Maria Teresa Gomes
dc.subject.por.fl_str_mv Malpighia emarginata; genetic variation; enzymatic analysis; genetic inheritance
Malpighia emarginata; variação genética; análise enzimática; herança genética
topic Malpighia emarginata; genetic variation; enzymatic analysis; genetic inheritance
Malpighia emarginata; variação genética; análise enzimática; herança genética
description The West Indian Cherry (Malpighia emarginata DC.) is a crop in expansion in Brazil mainly due to high fruit vitamin C content. However, there is still little information about the species. The objective of this study was to determine the activity and the polymorphism of isozymes from a collection of West Indian Cherry plants. IDH, MDH, EST, POD, ACP, GOT, PGM and PGI isozymes were studied. Starch gel horizontal electrophoresis and different gel/electrode buffers were used. Number of loci and alleles involved in genetic control of these isozymes were identified. IDH was dimeric and had one locus with two alleles. PGM was monomeric and had two loci, with two and four alleles. POD and EST were monomeric and had one and two loci, respectively, all with two alleles each. MDH had two polymorphic loci, one which was monomeric and one dimeric, both with two alleles each. GOT was polymorphic but its inheritance and genetic control was not determined. Polymorphisms of IDH, MDH, POD, EST, GOT and PGM showed potential as genetic markers in West Indian Cherry in studies of conservation and breeding.
publishDate 2002
dc.date.none.fl_str_mv 2002-02-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6322
url https://seer.sct.embrapa.br/index.php/pab/article/view/6322
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6322/3379
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.37, n.2, fev. 2002; 151-158
Pesquisa Agropecuária Brasileira; v.37, n.2, fev. 2002; 151-158
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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