Chemical sterilization of culture medium for anthurium in vitro culture
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/2122 |
Resumo: | The objective of this study was to evaluate the use of chlorine dioxide or of peracetic acid in the chemical sterilization of culture medium and in the development of anthurium (Anthurium andraeanum) plantlets cultured in vitro. Treatments consisted of chlorine dioxide and peracetic acid at 0.005 and 0.010%. The autoclaved medium was used as control. The anthurium plantlets were grown in elongation and rooting MS medium with the concentration of macronutrients reduced by half. The peracetic acid, in the concentrations used, was not effective in the sterilization of culture medium. The chlorine dioxide had 100% efficiency in the sterilization of culture medium and caused no phytotoxicity to plantlets. |
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Chemical sterilization of culture medium for anthurium in vitro cultureEsterilização química de meio de cultura no cultivo in vitro de antúrioAnthurium andraeanum; peracetic acid; chlorine dioxide; sterilization; micropropagationAnthurium andraeanum; ácido peracético; dióxido de cloro; esterilização; micropropagaçãoThe objective of this study was to evaluate the use of chlorine dioxide or of peracetic acid in the chemical sterilization of culture medium and in the development of anthurium (Anthurium andraeanum) plantlets cultured in vitro. Treatments consisted of chlorine dioxide and peracetic acid at 0.005 and 0.010%. The autoclaved medium was used as control. The anthurium plantlets were grown in elongation and rooting MS medium with the concentration of macronutrients reduced by half. The peracetic acid, in the concentrations used, was not effective in the sterilization of culture medium. The chlorine dioxide had 100% efficiency in the sterilization of culture medium and caused no phytotoxicity to plantlets.O objetivo deste trabalho foi avaliar o uso do dióxido de cloro ou do ácido peracético na esterilização química de meio de cultura e no desenvolvimento de mudas de antúrio (Anthurium andraeanum) cultivadas in vitro. Os tratamentos consistiram de dióxido de cloro e ácido peracético a 0,005 e 0,010%. Foi utilizado como controle o meio autoclavado. As mudas de antúrio foram cultivadas em meio de alongamento e enraizamento MS, com a concentração de nutrientes reduzida pela metade. O ácido peracético, nas concentrações utilizadas, não foi efetivo na esterilização do meio de cultura; o dióxido de cloro, porém, teve eficiência de 100%, sem causar fitotoxicidade às mudas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCardoso, Jean Carlos2010-11-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/2122Pesquisa Agropecuaria Brasileira; v.44, n.7, jul. 2009; 785-788Pesquisa Agropecuária Brasileira; v.44, n.7, jul. 2009; 785-7881678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/2122/5763https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2122/1515info:eu-repo/semantics/openAccess2012-06-17T11:50:46Zoai:ojs.seer.sct.embrapa.br:article/2122Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:50:46Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Chemical sterilization of culture medium for anthurium in vitro culture Esterilização química de meio de cultura no cultivo in vitro de antúrio |
title |
Chemical sterilization of culture medium for anthurium in vitro culture |
spellingShingle |
Chemical sterilization of culture medium for anthurium in vitro culture Cardoso, Jean Carlos Anthurium andraeanum; peracetic acid; chlorine dioxide; sterilization; micropropagation Anthurium andraeanum; ácido peracético; dióxido de cloro; esterilização; micropropagação |
title_short |
Chemical sterilization of culture medium for anthurium in vitro culture |
title_full |
Chemical sterilization of culture medium for anthurium in vitro culture |
title_fullStr |
Chemical sterilization of culture medium for anthurium in vitro culture |
title_full_unstemmed |
Chemical sterilization of culture medium for anthurium in vitro culture |
title_sort |
Chemical sterilization of culture medium for anthurium in vitro culture |
author |
Cardoso, Jean Carlos |
author_facet |
Cardoso, Jean Carlos |
author_role |
author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Cardoso, Jean Carlos |
dc.subject.por.fl_str_mv |
Anthurium andraeanum; peracetic acid; chlorine dioxide; sterilization; micropropagation Anthurium andraeanum; ácido peracético; dióxido de cloro; esterilização; micropropagação |
topic |
Anthurium andraeanum; peracetic acid; chlorine dioxide; sterilization; micropropagation Anthurium andraeanum; ácido peracético; dióxido de cloro; esterilização; micropropagação |
description |
The objective of this study was to evaluate the use of chlorine dioxide or of peracetic acid in the chemical sterilization of culture medium and in the development of anthurium (Anthurium andraeanum) plantlets cultured in vitro. Treatments consisted of chlorine dioxide and peracetic acid at 0.005 and 0.010%. The autoclaved medium was used as control. The anthurium plantlets were grown in elongation and rooting MS medium with the concentration of macronutrients reduced by half. The peracetic acid, in the concentrations used, was not effective in the sterilization of culture medium. The chlorine dioxide had 100% efficiency in the sterilization of culture medium and caused no phytotoxicity to plantlets. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-18 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/2122 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/2122 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/2122/5763 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2122/1515 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.44, n.7, jul. 2009; 785-788 Pesquisa Agropecuária Brasileira; v.44, n.7, jul. 2009; 785-788 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
_version_ |
1793416665807978496 |