Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopicta

Detalhes bibliográficos
Autor(a) principal: Barbosa, Flávia Rabelo
Data de Publicação: 2014
Outros Autores: Moreira, Wellington Antonio, Santos, Gil
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/16252
Resumo: The effect of succesive transplantation in a rice medium on the virulence of Metarrhizium anisopliae (Metsch.) Sorokin was studied through a biossay with nymphs of Deois flavopicta Stal 1854 (Homoptera, Cercopidae). The strain E9, from Espirito Santo state, Brazil, and an isolate J-71, obtained from a natural infection in Deois flavopicta, in Jataí, GO, Brazil, were tested. In both cases spores were collected from artificially infected spittlebugs to obtain the first generation in rice medium. It was demonstrated that the maximum degree of virulence was attained in the third generation of fungus. In this generation, the percentage of dead nymphs, due to Metarrhizium anisopliae, ranged from 65% for strain E9 to 76.6% for the J-71 isolate. After three generations in a rice medium, rapid reduction was observed in the nymphal infection rate. After 10 generations, the average percent infection was 11.3 for strain E9 and 16.7 for J-71. Insects started to die two days after the fungal inoculations; superficial mycelial growth began after three to four days, and sporulation started from five to seven days after inoculation.
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spelling Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopictaEfeito de sucessivas repicagens em arroz na virulência de Metarrhizium anisopliae para Deois flavopictaMetarrhizium anisopliae; Deois flavopicta; microbial control; grass spittlebugMetarrhizium anisopliae; Deois flavopicta; controle microbiológico; cigarrinha-das-pastagensThe effect of succesive transplantation in a rice medium on the virulence of Metarrhizium anisopliae (Metsch.) Sorokin was studied through a biossay with nymphs of Deois flavopicta Stal 1854 (Homoptera, Cercopidae). The strain E9, from Espirito Santo state, Brazil, and an isolate J-71, obtained from a natural infection in Deois flavopicta, in Jataí, GO, Brazil, were tested. In both cases spores were collected from artificially infected spittlebugs to obtain the first generation in rice medium. It was demonstrated that the maximum degree of virulence was attained in the third generation of fungus. In this generation, the percentage of dead nymphs, due to Metarrhizium anisopliae, ranged from 65% for strain E9 to 76.6% for the J-71 isolate. After three generations in a rice medium, rapid reduction was observed in the nymphal infection rate. After 10 generations, the average percent infection was 11.3 for strain E9 and 16.7 for J-71. Insects started to die two days after the fungal inoculations; superficial mycelial growth began after three to four days, and sporulation started from five to seven days after inoculation.Foi verificado, através de bioensaios com ninfas de Deois flavopicta Stal 1854 (Homoptera, Cercopidae), o efeito de sucessivas repicagens em meio de arroz, na virulência de Metarrihizium anisopliae (Metsch.) Sorokin. Testaram-se a cepa E9, proveniente do Estado do Espírito Santo, e o isolado J-71, obtido de infecção natural em D. flavopicta, em Jataí, GO. Em ambos casos, partiu-se de esporos retirados de cigarrinhas infectadas em laboratório, para obtenção da primeira geração em meio de arroz. Os resultados indicaram que o ponto máximo de virulência é atingido na terceira geração do fungo. Nesta geração, a percentagem de infecção das ninfas foi de 65% para a cepa E9, e 76,6% para o isolado J-71. A partir da terceira geração, houve sensível redução na infecção das ninfas; na décima geração, a percentagem de infecção foi de 11,3% para E9, e 16,7 para J-71. Dois dias após a inoculação do fungo, teve início a morte dos insetos. O crescimento miceliar externo iniciou-se após três a quatro dias, e a esporulação, após cinco a sete dias.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBarbosa, Flávia RabeloMoreira, Wellington AntonioSantos, Gil2014-04-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/16252Pesquisa Agropecuaria Brasileira; v.20, n.10, out. 1985; 1115-1118Pesquisa Agropecuária Brasileira; v.20, n.10, out. 1985; 1115-11181678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/16252/10433info:eu-repo/semantics/openAccess2014-04-16T19:33:05Zoai:ojs.seer.sct.embrapa.br:article/16252Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-16T19:33:05Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopicta
Efeito de sucessivas repicagens em arroz na virulência de Metarrhizium anisopliae para Deois flavopicta
title Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopicta
spellingShingle Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopicta
Barbosa, Flávia Rabelo
Metarrhizium anisopliae; Deois flavopicta; microbial control; grass spittlebug
Metarrhizium anisopliae; Deois flavopicta; controle microbiológico; cigarrinha-das-pastagens
title_short Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopicta
title_full Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopicta
title_fullStr Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopicta
title_full_unstemmed Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopicta
title_sort Effect of successive transplantation in rice on the virulence of Metarrhizium anisopliae (metsch.) sorokin against Deois flavopicta
author Barbosa, Flávia Rabelo
author_facet Barbosa, Flávia Rabelo
Moreira, Wellington Antonio
Santos, Gil
author_role author
author2 Moreira, Wellington Antonio
Santos, Gil
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Barbosa, Flávia Rabelo
Moreira, Wellington Antonio
Santos, Gil
dc.subject.por.fl_str_mv Metarrhizium anisopliae; Deois flavopicta; microbial control; grass spittlebug
Metarrhizium anisopliae; Deois flavopicta; controle microbiológico; cigarrinha-das-pastagens
topic Metarrhizium anisopliae; Deois flavopicta; microbial control; grass spittlebug
Metarrhizium anisopliae; Deois flavopicta; controle microbiológico; cigarrinha-das-pastagens
description The effect of succesive transplantation in a rice medium on the virulence of Metarrhizium anisopliae (Metsch.) Sorokin was studied through a biossay with nymphs of Deois flavopicta Stal 1854 (Homoptera, Cercopidae). The strain E9, from Espirito Santo state, Brazil, and an isolate J-71, obtained from a natural infection in Deois flavopicta, in Jataí, GO, Brazil, were tested. In both cases spores were collected from artificially infected spittlebugs to obtain the first generation in rice medium. It was demonstrated that the maximum degree of virulence was attained in the third generation of fungus. In this generation, the percentage of dead nymphs, due to Metarrhizium anisopliae, ranged from 65% for strain E9 to 76.6% for the J-71 isolate. After three generations in a rice medium, rapid reduction was observed in the nymphal infection rate. After 10 generations, the average percent infection was 11.3 for strain E9 and 16.7 for J-71. Insects started to die two days after the fungal inoculations; superficial mycelial growth began after three to four days, and sporulation started from five to seven days after inoculation.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-16
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/16252
url https://seer.sct.embrapa.br/index.php/pab/article/view/16252
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/16252/10433
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.20, n.10, out. 1985; 1115-1118
Pesquisa Agropecuária Brasileira; v.20, n.10, out. 1985; 1115-1118
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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