Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probiotic
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/392 |
Resumo: | The objective of this work was to isolate lactic-acid bacteria from the intestines of Nile tilapia, and to assess their potential as probiotic. Strains of lactic-acid bacteria were isolated, and inhibition againstpathogens was evaluated in vitro. Strains with best results were identified and used in tilapia intestinal tract colonization experiment through supplementation in the diet, in a completely randomized design, with three treatments and four replicates. Total number bacteria, lactic-acid bacteria, Vibrio ssp. and Pseudomonas ssp.were evaluated. The strain with best result was used for experimental infection, in a completely randomized design, in factorial arrangement 2x3: diet supplemented with strain and control diet; and fish not submitted to injection, fish submitted to injection of saline solution, and fish submitted to injection of Enterococcus durans, with three replicates. Hematological parameters were evaluated. Two strains were identified as Lactobacillus plantarum and Lactobacillus brevis, which colonized fishes’ intestinal tract. However, L. plantarum had thelowest number of total bacteria and of Pseudomonas ssp. Greater number of red blood cells, thrombocytes, leukocytes, lymphocytes, neutrophils and monocytes were observed in fish fed with L. plantarum and submittedto injection of E. durans. L. plantarum has probiotic effect on Nile tilapias and improve their immune system. |
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Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probioticUtilização de bactérias ácido-lácticas isoladas do trato intestinal de tilápia‑do‑nilo como probióticoEnterococcus durans; Lactobacillus plantarum; Oreochromis niloticus; hematology; experimental infectionEnterococcus durans; Lactobacillus plantarum; Oreochromis niloticus; hematologia; infecção experimentalThe objective of this work was to isolate lactic-acid bacteria from the intestines of Nile tilapia, and to assess their potential as probiotic. Strains of lactic-acid bacteria were isolated, and inhibition againstpathogens was evaluated in vitro. Strains with best results were identified and used in tilapia intestinal tract colonization experiment through supplementation in the diet, in a completely randomized design, with three treatments and four replicates. Total number bacteria, lactic-acid bacteria, Vibrio ssp. and Pseudomonas ssp.were evaluated. The strain with best result was used for experimental infection, in a completely randomized design, in factorial arrangement 2x3: diet supplemented with strain and control diet; and fish not submitted to injection, fish submitted to injection of saline solution, and fish submitted to injection of Enterococcus durans, with three replicates. Hematological parameters were evaluated. Two strains were identified as Lactobacillus plantarum and Lactobacillus brevis, which colonized fishes’ intestinal tract. However, L. plantarum had thelowest number of total bacteria and of Pseudomonas ssp. Greater number of red blood cells, thrombocytes, leukocytes, lymphocytes, neutrophils and monocytes were observed in fish fed with L. plantarum and submittedto injection of E. durans. L. plantarum has probiotic effect on Nile tilapias and improve their immune system.O objetivo deste trabalho foi isolar bactérias ácido-lácticas do intestino de tilápias-do-nilo, e avaliar seu potencial probiótico. Foram isoladas cepas de bactérias ácido-lácticas, e foi avaliada a inibição aospatógenos in vitro. As cepas com os melhores resultados foram identificadas e utilizadas no experimento de colonização do trato intestinal de tilápias-do-nilo, via suplementação na dieta, em delineamento inteiramente ao acaso, com três tratamentos e quatro repetições. Foram avaliados: o total de bactérias, as bactérias ácidolácticas, Vibrio ssp. e Pseudomonas ssp. A cepa com melhor resultado foi utilizada na infecção experimental, em delineamento inteiramente ao acaso, em esquema fatorial 2x3: dieta suplementada com a cepa e dietacontrole; e os peixes não submetidos à injeção, peixes submetidos à injeção de solução salina e à injeçãode Enterococcus durans, com três repetições. Foram avaliados os parâmetros hematológicos. As duas cepas identificadas foram: Lactobacillus plantarum e Lactobacillus brevis, que colonizaram o trato intestinal de tilápias, contudo L. plantarum teve menor número total de bactérias e de Pseudomonas ssp. Foi observadomaior número total de eritrócitos, trombócitos, leucócitos, linfócitos, neutrófilos e monócitos, em peixes alimentados com L. plantarum e submetidos à injeção de E. durans. O L. plantarum tem efeito probiótico e melhora o sistema imune das tilápias.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBIBIC/CnpqJatobá, AdolfoVieira, Felipe do NascimentoNeto, Celso BuglioneSilva, Bruno CorrêaMouriño, José Luís PedreiraJerônimo, Gabriela TomasDotta, GeovanaMartins, Maurício Laterça2008-10-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/392Pesquisa Agropecuaria Brasileira; v.43, n.9, set. 2008; 1201-1207Pesquisa Agropecuária Brasileira; v.43, n.9, set. 2008; 1201-12071678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/392/5501info:eu-repo/semantics/openAccess2014-07-16T17:36:33Zoai:ojs.seer.sct.embrapa.br:article/392Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-16T17:36:33Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probiotic Utilização de bactérias ácido-lácticas isoladas do trato intestinal de tilápia‑do‑nilo como probiótico |
title |
Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probiotic |
spellingShingle |
Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probiotic Jatobá, Adolfo Enterococcus durans; Lactobacillus plantarum; Oreochromis niloticus; hematology; experimental infection Enterococcus durans; Lactobacillus plantarum; Oreochromis niloticus; hematologia; infecção experimental |
title_short |
Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probiotic |
title_full |
Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probiotic |
title_fullStr |
Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probiotic |
title_full_unstemmed |
Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probiotic |
title_sort |
Lactic-acid bacteria isolated from the intestinal tract of Nile tilapia utilized as probiotic |
author |
Jatobá, Adolfo |
author_facet |
Jatobá, Adolfo Vieira, Felipe do Nascimento Neto, Celso Buglione Silva, Bruno Corrêa Mouriño, José Luís Pedreira Jerônimo, Gabriela Tomas Dotta, Geovana Martins, Maurício Laterça |
author_role |
author |
author2 |
Vieira, Felipe do Nascimento Neto, Celso Buglione Silva, Bruno Corrêa Mouriño, José Luís Pedreira Jerônimo, Gabriela Tomas Dotta, Geovana Martins, Maurício Laterça |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
BIBIC/Cnpq |
dc.contributor.author.fl_str_mv |
Jatobá, Adolfo Vieira, Felipe do Nascimento Neto, Celso Buglione Silva, Bruno Corrêa Mouriño, José Luís Pedreira Jerônimo, Gabriela Tomas Dotta, Geovana Martins, Maurício Laterça |
dc.subject.por.fl_str_mv |
Enterococcus durans; Lactobacillus plantarum; Oreochromis niloticus; hematology; experimental infection Enterococcus durans; Lactobacillus plantarum; Oreochromis niloticus; hematologia; infecção experimental |
topic |
Enterococcus durans; Lactobacillus plantarum; Oreochromis niloticus; hematology; experimental infection Enterococcus durans; Lactobacillus plantarum; Oreochromis niloticus; hematologia; infecção experimental |
description |
The objective of this work was to isolate lactic-acid bacteria from the intestines of Nile tilapia, and to assess their potential as probiotic. Strains of lactic-acid bacteria were isolated, and inhibition againstpathogens was evaluated in vitro. Strains with best results were identified and used in tilapia intestinal tract colonization experiment through supplementation in the diet, in a completely randomized design, with three treatments and four replicates. Total number bacteria, lactic-acid bacteria, Vibrio ssp. and Pseudomonas ssp.were evaluated. The strain with best result was used for experimental infection, in a completely randomized design, in factorial arrangement 2x3: diet supplemented with strain and control diet; and fish not submitted to injection, fish submitted to injection of saline solution, and fish submitted to injection of Enterococcus durans, with three replicates. Hematological parameters were evaluated. Two strains were identified as Lactobacillus plantarum and Lactobacillus brevis, which colonized fishes’ intestinal tract. However, L. plantarum had thelowest number of total bacteria and of Pseudomonas ssp. Greater number of red blood cells, thrombocytes, leukocytes, lymphocytes, neutrophils and monocytes were observed in fish fed with L. plantarum and submittedto injection of E. durans. L. plantarum has probiotic effect on Nile tilapias and improve their immune system. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-29 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/392 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/392 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/392/5501 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.43, n.9, set. 2008; 1201-1207 Pesquisa Agropecuária Brasileira; v.43, n.9, set. 2008; 1201-1207 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416668946366464 |